Collagen type XI 1 may be involved in the structural plasticity of the vertebral coulm in Atlantic salmon (Salmo salar L.)
First published online March 12, 2010. Journal homepage: http://jeb.biologists.org/ Atlantic salmon (Salmo salar L.) vertebral bone displays plasticity in structure, osteoid secretion and mineralization in response to photoperiod. Other properties of the vertebral bone, such as mineral content and m...
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Online Access: | http://hdl.handle.net/11250/117043 https://doi.org/10.1242/jeb.040022 |
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ftimr:oai:imr.brage.unit.no:11250/117043 2023-05-15T15:30:15+02:00 Collagen type XI 1 may be involved in the structural plasticity of the vertebral coulm in Atlantic salmon (Salmo salar L.) Wargelius, Anna Fjelldal, Per Gunnar Nordgarden, Ulla Grini, Ane Krossøy, B. Grotmol, Sindre Totland, Geir K. Hansen, Tom 2010 application/pdf http://hdl.handle.net/11250/117043 https://doi.org/10.1242/jeb.040022 eng eng The Company of Biologists urn:issn:1477-9145 urn:issn:0022-0949 http://hdl.handle.net/11250/117043 http://dx.doi.org/10.1242/jeb.040022 1207-1216 213 Journal of Experimental Biology atlantic salmon atlantisk laks VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Journal article Peer reviewed 2010 ftimr https://doi.org/10.1242/jeb.040022 2021-09-23T20:15:03Z First published online March 12, 2010. Journal homepage: http://jeb.biologists.org/ Atlantic salmon (Salmo salar L.) vertebral bone displays plasticity in structure, osteoid secretion and mineralization in response to photoperiod. Other properties of the vertebral bone, such as mineral content and mechanical strength, are also associated with common malformations in farmed Atlantic salmon. The biological mechanisms that underlie these changes in bone physiology are unknown, and in order to elucidate which factors might be involved in this process, microarray assays were performed on vertebral bone of Atlantic salmon reared under natural or continuous light. Eight genes were upregulated in response to continuous light treatment, whereas only one of them was upregulated in a duplicate experiment. The transcriptionally regulated gene was predicted to code for collagen type XI a1, a protein known to be involved in controlling the diameter of fibrillar collagens in mammals. Furthermore, the gene was highly expressed in the vertebrae, where spatial expression was found in becular and compact bone osteoblasts and in the chordoblasts of the notochordal sheath. When we measured the expression level of the gene in the tissue compartments of the vertebrae, the collagen turned out to be 150 and 25 times more highly expressed in the notochord and compact bone respectively, relative to the expression in the trabecular bone. Gene expression was induced in response to continuous light, and reduced in compressed vertebrae. The downregulation in compressed vertebrae was due to reduced expression in the compact bone, while expression in the trabecular bone and the notochord was unaffected. These data support the hypothesis that this gene codes for a presumptive collagen type XI a1, which may be involved in the regulatory pathway leading to structural adaptation of the vertebral architecture. Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR Journal of Experimental Biology 213 7 1207 1216 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
topic |
atlantic salmon atlantisk laks VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 |
spellingShingle |
atlantic salmon atlantisk laks VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Wargelius, Anna Fjelldal, Per Gunnar Nordgarden, Ulla Grini, Ane Krossøy, B. Grotmol, Sindre Totland, Geir K. Hansen, Tom Collagen type XI 1 may be involved in the structural plasticity of the vertebral coulm in Atlantic salmon (Salmo salar L.) |
topic_facet |
atlantic salmon atlantisk laks VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 |
description |
First published online March 12, 2010. Journal homepage: http://jeb.biologists.org/ Atlantic salmon (Salmo salar L.) vertebral bone displays plasticity in structure, osteoid secretion and mineralization in response to photoperiod. Other properties of the vertebral bone, such as mineral content and mechanical strength, are also associated with common malformations in farmed Atlantic salmon. The biological mechanisms that underlie these changes in bone physiology are unknown, and in order to elucidate which factors might be involved in this process, microarray assays were performed on vertebral bone of Atlantic salmon reared under natural or continuous light. Eight genes were upregulated in response to continuous light treatment, whereas only one of them was upregulated in a duplicate experiment. The transcriptionally regulated gene was predicted to code for collagen type XI a1, a protein known to be involved in controlling the diameter of fibrillar collagens in mammals. Furthermore, the gene was highly expressed in the vertebrae, where spatial expression was found in becular and compact bone osteoblasts and in the chordoblasts of the notochordal sheath. When we measured the expression level of the gene in the tissue compartments of the vertebrae, the collagen turned out to be 150 and 25 times more highly expressed in the notochord and compact bone respectively, relative to the expression in the trabecular bone. Gene expression was induced in response to continuous light, and reduced in compressed vertebrae. The downregulation in compressed vertebrae was due to reduced expression in the compact bone, while expression in the trabecular bone and the notochord was unaffected. These data support the hypothesis that this gene codes for a presumptive collagen type XI a1, which may be involved in the regulatory pathway leading to structural adaptation of the vertebral architecture. |
format |
Article in Journal/Newspaper |
author |
Wargelius, Anna Fjelldal, Per Gunnar Nordgarden, Ulla Grini, Ane Krossøy, B. Grotmol, Sindre Totland, Geir K. Hansen, Tom |
author_facet |
Wargelius, Anna Fjelldal, Per Gunnar Nordgarden, Ulla Grini, Ane Krossøy, B. Grotmol, Sindre Totland, Geir K. Hansen, Tom |
author_sort |
Wargelius, Anna |
title |
Collagen type XI 1 may be involved in the structural plasticity of the vertebral coulm in Atlantic salmon (Salmo salar L.) |
title_short |
Collagen type XI 1 may be involved in the structural plasticity of the vertebral coulm in Atlantic salmon (Salmo salar L.) |
title_full |
Collagen type XI 1 may be involved in the structural plasticity of the vertebral coulm in Atlantic salmon (Salmo salar L.) |
title_fullStr |
Collagen type XI 1 may be involved in the structural plasticity of the vertebral coulm in Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Collagen type XI 1 may be involved in the structural plasticity of the vertebral coulm in Atlantic salmon (Salmo salar L.) |
title_sort |
collagen type xi 1 may be involved in the structural plasticity of the vertebral coulm in atlantic salmon (salmo salar l.) |
publisher |
The Company of Biologists |
publishDate |
2010 |
url |
http://hdl.handle.net/11250/117043 https://doi.org/10.1242/jeb.040022 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
1207-1216 213 Journal of Experimental Biology |
op_relation |
urn:issn:1477-9145 urn:issn:0022-0949 http://hdl.handle.net/11250/117043 http://dx.doi.org/10.1242/jeb.040022 |
op_doi |
https://doi.org/10.1242/jeb.040022 |
container_title |
Journal of Experimental Biology |
container_volume |
213 |
container_issue |
7 |
container_start_page |
1207 |
op_container_end_page |
1216 |
_version_ |
1766360688476815360 |