Viral encephalopathy and retinopathy (VER) in Atlantic salmon Salmo salar after intraperitoneal challenge with a nodavirus from Atlantic halibut Hippoglossus hippoglossus
Homogenate of tissue from juveniles of Atlantic halibut Hippoglossus hippoglossus suffering from viral encephalopathy and retinopathy (VER) was used to challenge smolt of Atlantic salmon Salmo salar with an initial average weight of 110 g. The nodavirus was administered in the form of an intraperito...
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Online Access: | http://hdl.handle.net/11250/109126 https://doi.org/10.3354/dao068007 |
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ftimr:oai:imr.brage.unit.no:11250/109126 2024-09-15T17:56:08+00:00 Viral encephalopathy and retinopathy (VER) in Atlantic salmon Salmo salar after intraperitoneal challenge with a nodavirus from Atlantic halibut Hippoglossus hippoglossus Korsnes, Kjetil Devold, Magnus Nerland, Audun H. Nylund, Are 2005-12-30 application/pdf http://hdl.handle.net/11250/109126 https://doi.org/10.3354/dao068007 eng eng Inter-Research urn:issn:0177-5103 http://hdl.handle.net/11250/109126 http://dx.doi.org/10.3354/dao068007 7-15 68 Diseases of Aquatic Organisms 1 atlantisk laks atlantic salmon halibut kveite nodavirus VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Journal article Peer reviewed 2005 ftimr https://doi.org/10.3354/dao068007 2024-07-31T03:37:25Z Homogenate of tissue from juveniles of Atlantic halibut Hippoglossus hippoglossus suffering from viral encephalopathy and retinopathy (VER) was used to challenge smolt of Atlantic salmon Salmo salar with an initial average weight of 110 g. The nodavirus was administered in the form of an intraperitoneal injection, and the fish were kept for 134 d post challenge. Genotype characterisation of the nodavirus was performed by sequencing the RNA1 and RNA2 segments, and a quantitative real-time PCR (Q-PCR) assay was developed. Tissues from different organs were stained by immunohistochemistry (IHC). Samples were collected at random on Days 7, 25, 45, 69, 125 and 134 after challenge. Mortality, clinical signs and pathology of VER were observed only in the challenged group. The Q-PCR detected positive fish only in the challenged group, all of which were positive on all days of sampling. An increase in relative virus concentrations was observed from Day 7 to Day 25 post challenge. The increased level of virus concentration was maintained in the medulla oblongata throughout the experiment, suggesting persistence or slow elimination of the virus over time. The IHC detected positive cells on Days 34, 70 and 74. These results suggest that the nodavirus is transported to the medulla oblongata from the intraperitoneal injection site and is able to replicate in salmon. When injected, this nodavirus isolate caused mortality and established a persistent infection in the challenged salmon throughout the experiment. This susceptibility suggests that co-location of salmon and marine species should be avoided until further studies of possible transmission have been carried out. Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR Diseases of Aquatic Organisms 68 7 15 |
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Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
topic |
atlantisk laks atlantic salmon halibut kveite nodavirus VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 |
spellingShingle |
atlantisk laks atlantic salmon halibut kveite nodavirus VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Korsnes, Kjetil Devold, Magnus Nerland, Audun H. Nylund, Are Viral encephalopathy and retinopathy (VER) in Atlantic salmon Salmo salar after intraperitoneal challenge with a nodavirus from Atlantic halibut Hippoglossus hippoglossus |
topic_facet |
atlantisk laks atlantic salmon halibut kveite nodavirus VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 |
description |
Homogenate of tissue from juveniles of Atlantic halibut Hippoglossus hippoglossus suffering from viral encephalopathy and retinopathy (VER) was used to challenge smolt of Atlantic salmon Salmo salar with an initial average weight of 110 g. The nodavirus was administered in the form of an intraperitoneal injection, and the fish were kept for 134 d post challenge. Genotype characterisation of the nodavirus was performed by sequencing the RNA1 and RNA2 segments, and a quantitative real-time PCR (Q-PCR) assay was developed. Tissues from different organs were stained by immunohistochemistry (IHC). Samples were collected at random on Days 7, 25, 45, 69, 125 and 134 after challenge. Mortality, clinical signs and pathology of VER were observed only in the challenged group. The Q-PCR detected positive fish only in the challenged group, all of which were positive on all days of sampling. An increase in relative virus concentrations was observed from Day 7 to Day 25 post challenge. The increased level of virus concentration was maintained in the medulla oblongata throughout the experiment, suggesting persistence or slow elimination of the virus over time. The IHC detected positive cells on Days 34, 70 and 74. These results suggest that the nodavirus is transported to the medulla oblongata from the intraperitoneal injection site and is able to replicate in salmon. When injected, this nodavirus isolate caused mortality and established a persistent infection in the challenged salmon throughout the experiment. This susceptibility suggests that co-location of salmon and marine species should be avoided until further studies of possible transmission have been carried out. |
format |
Article in Journal/Newspaper |
author |
Korsnes, Kjetil Devold, Magnus Nerland, Audun H. Nylund, Are |
author_facet |
Korsnes, Kjetil Devold, Magnus Nerland, Audun H. Nylund, Are |
author_sort |
Korsnes, Kjetil |
title |
Viral encephalopathy and retinopathy (VER) in Atlantic salmon Salmo salar after intraperitoneal challenge with a nodavirus from Atlantic halibut Hippoglossus hippoglossus |
title_short |
Viral encephalopathy and retinopathy (VER) in Atlantic salmon Salmo salar after intraperitoneal challenge with a nodavirus from Atlantic halibut Hippoglossus hippoglossus |
title_full |
Viral encephalopathy and retinopathy (VER) in Atlantic salmon Salmo salar after intraperitoneal challenge with a nodavirus from Atlantic halibut Hippoglossus hippoglossus |
title_fullStr |
Viral encephalopathy and retinopathy (VER) in Atlantic salmon Salmo salar after intraperitoneal challenge with a nodavirus from Atlantic halibut Hippoglossus hippoglossus |
title_full_unstemmed |
Viral encephalopathy and retinopathy (VER) in Atlantic salmon Salmo salar after intraperitoneal challenge with a nodavirus from Atlantic halibut Hippoglossus hippoglossus |
title_sort |
viral encephalopathy and retinopathy (ver) in atlantic salmon salmo salar after intraperitoneal challenge with a nodavirus from atlantic halibut hippoglossus hippoglossus |
publisher |
Inter-Research |
publishDate |
2005 |
url |
http://hdl.handle.net/11250/109126 https://doi.org/10.3354/dao068007 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
7-15 68 Diseases of Aquatic Organisms 1 |
op_relation |
urn:issn:0177-5103 http://hdl.handle.net/11250/109126 http://dx.doi.org/10.3354/dao068007 |
op_doi |
https://doi.org/10.3354/dao068007 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
68 |
container_start_page |
7 |
op_container_end_page |
15 |
_version_ |
1810432350306172928 |