Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS)
The present study shows that a nodavirus-like agent is associated with the lesions of cardiac myopathy syndrome (CMS), a disease of unknown etiology which affects reared adult Atlantic salmon Salmo salar. In archive paraffin-embedded heart tissue from Atlantic salmon diagnosed as suffering from CMS,...
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Language: | English |
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1997
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Online Access: | http://hdl.handle.net/11250/108410 https://doi.org/10.3354/dao029079 |
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ftimr:oai:imr.brage.unit.no:11250/108410 2023-05-15T15:30:10+02:00 Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS) Grotmol, Sindre Totland, Geir K. Kryvi, Harald 1997-05-22 3292028 bytes application/pdf http://hdl.handle.net/11250/108410 https://doi.org/10.3354/dao029079 eng eng urn:issn:0177-5103 http://hdl.handle.net/11250/108410 http://dx.doi.org/10.3354/dao029079 79-84 29 Diseases of Aquatic Organisms 2 salmon laks nodavirus Journal article Peer reviewed 1997 ftimr https://doi.org/10.3354/dao029079 2021-09-23T20:15:20Z The present study shows that a nodavirus-like agent is associated with the lesions of cardiac myopathy syndrome (CMS), a disease of unknown etiology which affects reared adult Atlantic salmon Salmo salar. In archive paraffin-embedded heart tissue from Atlantic salmon diagnosed as suffering from CMS, a distinct immunohistochemical reaction was observed when using a primary antibody against striped jack nervous necrosis virus (Nodaviridae). Immunolabeling was detected in the mesothelium and hypercellular lesions of the epicardium and in endothelial cells and myocytes within mild multifocal lesions of the atrial and ventricular trabeculae. Transmission electron microscopy, per formed on deparaffinized samples of the same tissue blocks, revealed substantial amounts of virus-like particles (VLP) in the cytoplasm of endocardial endothelium, in myocytes and in mesothelial cells of the epicardium. The VLP were isometric, spherical and unenveloped, with mean capsid diameters of approximately 25 nm, resembling a virus belonging to the Nodaviridae. Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR Diseases of Aquatic Organisms 29 79 84 |
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Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
topic |
salmon laks nodavirus |
spellingShingle |
salmon laks nodavirus Grotmol, Sindre Totland, Geir K. Kryvi, Harald Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS) |
topic_facet |
salmon laks nodavirus |
description |
The present study shows that a nodavirus-like agent is associated with the lesions of cardiac myopathy syndrome (CMS), a disease of unknown etiology which affects reared adult Atlantic salmon Salmo salar. In archive paraffin-embedded heart tissue from Atlantic salmon diagnosed as suffering from CMS, a distinct immunohistochemical reaction was observed when using a primary antibody against striped jack nervous necrosis virus (Nodaviridae). Immunolabeling was detected in the mesothelium and hypercellular lesions of the epicardium and in endothelial cells and myocytes within mild multifocal lesions of the atrial and ventricular trabeculae. Transmission electron microscopy, per formed on deparaffinized samples of the same tissue blocks, revealed substantial amounts of virus-like particles (VLP) in the cytoplasm of endocardial endothelium, in myocytes and in mesothelial cells of the epicardium. The VLP were isometric, spherical and unenveloped, with mean capsid diameters of approximately 25 nm, resembling a virus belonging to the Nodaviridae. |
format |
Article in Journal/Newspaper |
author |
Grotmol, Sindre Totland, Geir K. Kryvi, Harald |
author_facet |
Grotmol, Sindre Totland, Geir K. Kryvi, Harald |
author_sort |
Grotmol, Sindre |
title |
Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS) |
title_short |
Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS) |
title_full |
Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS) |
title_fullStr |
Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS) |
title_full_unstemmed |
Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS) |
title_sort |
detection of a nodavirus-like agent in heart tissue from reared atlantic salmon salmo salar suffering from cardiac myopathy syndrome (cms) |
publishDate |
1997 |
url |
http://hdl.handle.net/11250/108410 https://doi.org/10.3354/dao029079 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
79-84 29 Diseases of Aquatic Organisms 2 |
op_relation |
urn:issn:0177-5103 http://hdl.handle.net/11250/108410 http://dx.doi.org/10.3354/dao029079 |
op_doi |
https://doi.org/10.3354/dao029079 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
29 |
container_start_page |
79 |
op_container_end_page |
84 |
_version_ |
1766360618771677184 |