Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum
Farming of Atlantic cod Gadus morhua is one of the most rapidly growing sectors of Norwegian aquaculture. Classical vibriosis caused by Vibrio anguillarum is a problem in cod aquaculture. To prevent disease outbreaks, a thorough understanding of the infection route and the impact of the bacteria on...
Published in: | Diseases of Aquatic Organisms |
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Language: | English |
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2008
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Online Access: | http://hdl.handle.net/11250/108142 https://doi.org/10.3354/dao01926 |
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ftimr:oai:imr.brage.unit.no:11250/108142 2023-05-15T15:27:18+02:00 Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum Engelsen, Ane Rebecca Sandlund, Nina Fiksdal, Ingrid Uglenes Bergh, Øivind 2008-06-19 724648 bytes application/pdf http://hdl.handle.net/11250/108142 https://doi.org/10.3354/dao01926 eng eng urn:issn:0177-5103 urn:issn:1616-1580 http://hdl.handle.net/11250/108142 http://dx.doi.org/10.3354/dao01926 13-20 80 Diseases of Aquatic Organisms Journal article Peer reviewed 2008 ftimr https://doi.org/10.3354/dao01926 2021-09-23T20:15:44Z Farming of Atlantic cod Gadus morhua is one of the most rapidly growing sectors of Norwegian aquaculture. Classical vibriosis caused by Vibrio anguillarum is a problem in cod aquaculture. To prevent disease outbreaks, a thorough understanding of the infection route and the impact of the bacteria on the host is important. The intestinal tract, skin and gills have all been proposed as routes of entry for bacterial infections such as vibriosis. We aimed to further develop understanding of V. anguillarum serotype O2α infections in cod larvae by elucidation of a possible route of entry, the pattern of infection and its histopathology. Cod eggs were transferred to a 24-well polystyrene multi-dish with 2 ml of sterile aerated 80% (28‰ salinity) seawater. Challenge doses were 104 and 106 CFU ml–1. Unchallenged larvae were used as controls. Larvae for immunohistochemical examination were sampled daily from each group. In most of the larvae, either no or very few bacteria were observed. Typical findings were clusters of bacteria in the spaces between the primary gill lamellae. None of these bacteria seemed to have adhered to the gills. Intestines of 3 out of 161 larvae examined contained positively immunostained bacteria. Some bacteria appeared attached to the microvilli, but none was observed inside epithelial cells. Only 2 larvae from the low-challenge dose group showed clear signs of histopathology, which occurred in the intestine. It is not possible to draw any conclusions regarding the portal of entry. Article in Journal/Newspaper atlantic cod Gadus morhua Institute for Marine Research: Brage IMR The Portal ENVELOPE(159.167,159.167,-78.100,-78.100) Diseases of Aquatic Organisms 80 13 20 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
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ftimr |
language |
English |
description |
Farming of Atlantic cod Gadus morhua is one of the most rapidly growing sectors of Norwegian aquaculture. Classical vibriosis caused by Vibrio anguillarum is a problem in cod aquaculture. To prevent disease outbreaks, a thorough understanding of the infection route and the impact of the bacteria on the host is important. The intestinal tract, skin and gills have all been proposed as routes of entry for bacterial infections such as vibriosis. We aimed to further develop understanding of V. anguillarum serotype O2α infections in cod larvae by elucidation of a possible route of entry, the pattern of infection and its histopathology. Cod eggs were transferred to a 24-well polystyrene multi-dish with 2 ml of sterile aerated 80% (28‰ salinity) seawater. Challenge doses were 104 and 106 CFU ml–1. Unchallenged larvae were used as controls. Larvae for immunohistochemical examination were sampled daily from each group. In most of the larvae, either no or very few bacteria were observed. Typical findings were clusters of bacteria in the spaces between the primary gill lamellae. None of these bacteria seemed to have adhered to the gills. Intestines of 3 out of 161 larvae examined contained positively immunostained bacteria. Some bacteria appeared attached to the microvilli, but none was observed inside epithelial cells. Only 2 larvae from the low-challenge dose group showed clear signs of histopathology, which occurred in the intestine. It is not possible to draw any conclusions regarding the portal of entry. |
format |
Article in Journal/Newspaper |
author |
Engelsen, Ane Rebecca Sandlund, Nina Fiksdal, Ingrid Uglenes Bergh, Øivind |
spellingShingle |
Engelsen, Ane Rebecca Sandlund, Nina Fiksdal, Ingrid Uglenes Bergh, Øivind Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum |
author_facet |
Engelsen, Ane Rebecca Sandlund, Nina Fiksdal, Ingrid Uglenes Bergh, Øivind |
author_sort |
Engelsen, Ane Rebecca |
title |
Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum |
title_short |
Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum |
title_full |
Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum |
title_fullStr |
Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum |
title_full_unstemmed |
Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum |
title_sort |
immunohistochemistry of atlantic cod larvae gadus morhua experimentally challenged with vibrio anguillarum |
publishDate |
2008 |
url |
http://hdl.handle.net/11250/108142 https://doi.org/10.3354/dao01926 |
long_lat |
ENVELOPE(159.167,159.167,-78.100,-78.100) |
geographic |
The Portal |
geographic_facet |
The Portal |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
13-20 80 Diseases of Aquatic Organisms |
op_relation |
urn:issn:0177-5103 urn:issn:1616-1580 http://hdl.handle.net/11250/108142 http://dx.doi.org/10.3354/dao01926 |
op_doi |
https://doi.org/10.3354/dao01926 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
80 |
container_start_page |
13 |
op_container_end_page |
20 |
_version_ |
1766357754353549312 |