Zebrafish IL-4-like cytokines and IL-10 suppress inflammation but only IL-10 is essential for gill homeostasis
Mucosal surfaces such as fish gills interface between the organism and the external environment and as such are major sites of foreign Ag encounter. In the gills, the balance between inflammatory responses to waterborne pathogens and regulatory responses toward commensal microbes is critical for eff...
Published in: | The Journal of Immunology |
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American Association of Immunologists
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Online Access: | http://hdl.handle.net/10044/1/83214 https://doi.org/10.4049/jimmunol.2000372 |
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ftimperialcol:oai:spiral.imperial.ac.uk:10044/1/83214 2023-05-15T15:32:42+02:00 Zebrafish IL-4-like cytokines and IL-10 suppress inflammation but only IL-10 is essential for gill homeostasis Bottiglione, F Dee, CT Lea, R Zeef, LAH Badrock, AP Wane, M Bugeon, L Dallman, MJ Allen, JE Hurlstone, AFL 2020-06-09 http://hdl.handle.net/10044/1/83214 https://doi.org/10.4049/jimmunol.2000372 English eng American Association of Immunologists Journal of Immunology 0022-1767 http://hdl.handle.net/10044/1/83214 doi:10.4049/jimmunol.2000372 © 2020 The Authors This article is distributed under the terms of the CC BY 4.0 Unported license https://creativecommons.org/licenses/by/4.0/. https://creativecommons.org/licenses/by/4.0/ CC-BY 1008 994 Science & Technology Life Sciences & Biomedicine Immunology TROUT ONCORHYNCHUS-MYKISS SALMO-SALAR-L SET ENRICHMENT ANALYSIS ATLANTIC SALMON TYPE-2 IMMUNITY T-CELLS EXPRESSION ANALYSIS ADAPTIVE IMMUNITY LYMPHOID-TISSUE HEAD KIDNEY 1107 Immunology Journal Article 2020 ftimperialcol https://doi.org/10.4049/jimmunol.2000372 2020-10-15T22:38:22Z Mucosal surfaces such as fish gills interface between the organism and the external environment and as such are major sites of foreign Ag encounter. In the gills, the balance between inflammatory responses to waterborne pathogens and regulatory responses toward commensal microbes is critical for effective barrier function and overall fish health. In mammals, IL-4 and IL-13 in concert with IL-10 are essential for balancing immune responses to pathogens and suppressing inflammation. Although considerable progress has been made in the field of fish immunology in recent years, whether the fish counterparts of these key mammalian cytokines perform similar roles is still an open question. In this study, we have generated IL-4/13A and IL-4/13B mutant zebrafish (Danio rerio) and, together with an existing IL-10 mutant line, characterized the consequences of loss of function of these cytokines. We demonstrate that IL-4/13A and IL-4/13B are required for the maintenance of a Th2-like phenotype in the gills and the suppression of type 1 immune responses. As in mammals, IL-10 appears to have a more striking anti-inflammatory function than IL-4–like cytokines and is essential for gill homeostasis. Thus, both IL-4/13 and IL-10 paralogs in zebrafish exhibit aspects of conserved function with their mammalian counterparts. Article in Journal/Newspaper Atlantic salmon Salmo salar Imperial College London: Spiral The Journal of Immunology 205 4 994 1008 |
institution |
Open Polar |
collection |
Imperial College London: Spiral |
op_collection_id |
ftimperialcol |
language |
English |
topic |
Science & Technology Life Sciences & Biomedicine Immunology TROUT ONCORHYNCHUS-MYKISS SALMO-SALAR-L SET ENRICHMENT ANALYSIS ATLANTIC SALMON TYPE-2 IMMUNITY T-CELLS EXPRESSION ANALYSIS ADAPTIVE IMMUNITY LYMPHOID-TISSUE HEAD KIDNEY 1107 Immunology |
spellingShingle |
Science & Technology Life Sciences & Biomedicine Immunology TROUT ONCORHYNCHUS-MYKISS SALMO-SALAR-L SET ENRICHMENT ANALYSIS ATLANTIC SALMON TYPE-2 IMMUNITY T-CELLS EXPRESSION ANALYSIS ADAPTIVE IMMUNITY LYMPHOID-TISSUE HEAD KIDNEY 1107 Immunology Bottiglione, F Dee, CT Lea, R Zeef, LAH Badrock, AP Wane, M Bugeon, L Dallman, MJ Allen, JE Hurlstone, AFL Zebrafish IL-4-like cytokines and IL-10 suppress inflammation but only IL-10 is essential for gill homeostasis |
topic_facet |
Science & Technology Life Sciences & Biomedicine Immunology TROUT ONCORHYNCHUS-MYKISS SALMO-SALAR-L SET ENRICHMENT ANALYSIS ATLANTIC SALMON TYPE-2 IMMUNITY T-CELLS EXPRESSION ANALYSIS ADAPTIVE IMMUNITY LYMPHOID-TISSUE HEAD KIDNEY 1107 Immunology |
description |
Mucosal surfaces such as fish gills interface between the organism and the external environment and as such are major sites of foreign Ag encounter. In the gills, the balance between inflammatory responses to waterborne pathogens and regulatory responses toward commensal microbes is critical for effective barrier function and overall fish health. In mammals, IL-4 and IL-13 in concert with IL-10 are essential for balancing immune responses to pathogens and suppressing inflammation. Although considerable progress has been made in the field of fish immunology in recent years, whether the fish counterparts of these key mammalian cytokines perform similar roles is still an open question. In this study, we have generated IL-4/13A and IL-4/13B mutant zebrafish (Danio rerio) and, together with an existing IL-10 mutant line, characterized the consequences of loss of function of these cytokines. We demonstrate that IL-4/13A and IL-4/13B are required for the maintenance of a Th2-like phenotype in the gills and the suppression of type 1 immune responses. As in mammals, IL-10 appears to have a more striking anti-inflammatory function than IL-4–like cytokines and is essential for gill homeostasis. Thus, both IL-4/13 and IL-10 paralogs in zebrafish exhibit aspects of conserved function with their mammalian counterparts. |
format |
Article in Journal/Newspaper |
author |
Bottiglione, F Dee, CT Lea, R Zeef, LAH Badrock, AP Wane, M Bugeon, L Dallman, MJ Allen, JE Hurlstone, AFL |
author_facet |
Bottiglione, F Dee, CT Lea, R Zeef, LAH Badrock, AP Wane, M Bugeon, L Dallman, MJ Allen, JE Hurlstone, AFL |
author_sort |
Bottiglione, F |
title |
Zebrafish IL-4-like cytokines and IL-10 suppress inflammation but only IL-10 is essential for gill homeostasis |
title_short |
Zebrafish IL-4-like cytokines and IL-10 suppress inflammation but only IL-10 is essential for gill homeostasis |
title_full |
Zebrafish IL-4-like cytokines and IL-10 suppress inflammation but only IL-10 is essential for gill homeostasis |
title_fullStr |
Zebrafish IL-4-like cytokines and IL-10 suppress inflammation but only IL-10 is essential for gill homeostasis |
title_full_unstemmed |
Zebrafish IL-4-like cytokines and IL-10 suppress inflammation but only IL-10 is essential for gill homeostasis |
title_sort |
zebrafish il-4-like cytokines and il-10 suppress inflammation but only il-10 is essential for gill homeostasis |
publisher |
American Association of Immunologists |
publishDate |
2020 |
url |
http://hdl.handle.net/10044/1/83214 https://doi.org/10.4049/jimmunol.2000372 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
1008 994 |
op_relation |
Journal of Immunology 0022-1767 http://hdl.handle.net/10044/1/83214 doi:10.4049/jimmunol.2000372 |
op_rights |
© 2020 The Authors This article is distributed under the terms of the CC BY 4.0 Unported license https://creativecommons.org/licenses/by/4.0/. https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.4049/jimmunol.2000372 |
container_title |
The Journal of Immunology |
container_volume |
205 |
container_issue |
4 |
container_start_page |
994 |
op_container_end_page |
1008 |
_version_ |
1766363198044241920 |