Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss
L-Asparaginase (L-asparagine aminohydrolase, E.C. 3.5.1.1) has been proven to be competent in treating Acute Lymphoblastic Leukaemia (ALL), which is widely observed in paediatric and adult groups. Currently, clinical L-Asparaginase formulations are derived from bacterial sources such as Escherichia...
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ftiith:oai:raiith.iith.ac.in:4810 2023-05-15T14:06:36+02:00 Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss Ashok, Anup Doriya, Kruthi Rao, Jyothi Vithal Qureshi, Asif Tiwari, Anoop Kumar Kumar, Devarai Santhosh 2019 text http://raiith.iith.ac.in/4810/ https://doi.org/10.1038/s41598-018-38094-1 http://raiith.iith.ac.in/4810/1/Scientific%20Reports_9_1_1-10_2019.pdf en eng Nature Publishing Group http://raiith.iith.ac.in/4810/1/Scientific%20Reports_9_1_1-10_2019.pdf Ashok, Anup and Doriya, Kruthi and Rao, Jyothi Vithal and Qureshi, Asif and Tiwari, Anoop Kumar and Kumar, Devarai Santhosh (2019) Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss. Scientific Reports, 9 (1). pp. 1-10. ISSN 2045-2322 Civil Engineering Article PeerReviewed 2019 ftiith https://doi.org/10.1038/s41598-018-38094-1 2022-09-28T08:34:56Z L-Asparaginase (L-asparagine aminohydrolase, E.C. 3.5.1.1) has been proven to be competent in treating Acute Lymphoblastic Leukaemia (ALL), which is widely observed in paediatric and adult groups. Currently, clinical L-Asparaginase formulations are derived from bacterial sources such as Escherichia coli and Erwinia chrysanthemi. These formulations when administered to ALL patients lead to several immunological and hypersensitive reactions. Hence, additional purification steps are required to remove toxicity induced by the amalgamation of other enzymes like glutaminase and urease. Production of L-Asparaginase that is free of glutaminase and urease is a major area of research. In this paper, we report the screening and isolation of fungal species collected from the soil and mosses in the Schirmacher Hills, Dronning Maud Land, Antarctica, that produce L-Asparaginase free of glutaminase and urease. A total of 55 isolates were obtained from 33 environmental samples that were tested by conventional plate techniques using Phenol red and Bromothymol blue as indicators. Among the isolated fungi, 30 isolates showed L-Asparaginase free of glutaminase and urease. The L-Asparaginase producing strain Trichosporon asahii IBBLA1, which showed the highest zone index, was then optimized with a Taguchi design. Optimum enzyme activity of 20.57 U mL−1 was obtained at a temperature of 30 °C and pH of 7.0 after 60 hours. Our work suggests that isolation of fungi from extreme environments such as Antarctica may lead to an important advancement in therapeutic applications with fewer side effects. Article in Journal/Newspaper Antarc* Antarctic Antarctica Dronning Maud Land Research Archive of Indian Institute of Technology, Hyderabad (RAIITH) Antarctic Dronning Maud Land Schirmacher Hills ENVELOPE(11.667,11.667,-70.750,-70.750) Scientific Reports 9 1 |
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Research Archive of Indian Institute of Technology, Hyderabad (RAIITH) |
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language |
English |
topic |
Civil Engineering |
spellingShingle |
Civil Engineering Ashok, Anup Doriya, Kruthi Rao, Jyothi Vithal Qureshi, Asif Tiwari, Anoop Kumar Kumar, Devarai Santhosh Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss |
topic_facet |
Civil Engineering |
description |
L-Asparaginase (L-asparagine aminohydrolase, E.C. 3.5.1.1) has been proven to be competent in treating Acute Lymphoblastic Leukaemia (ALL), which is widely observed in paediatric and adult groups. Currently, clinical L-Asparaginase formulations are derived from bacterial sources such as Escherichia coli and Erwinia chrysanthemi. These formulations when administered to ALL patients lead to several immunological and hypersensitive reactions. Hence, additional purification steps are required to remove toxicity induced by the amalgamation of other enzymes like glutaminase and urease. Production of L-Asparaginase that is free of glutaminase and urease is a major area of research. In this paper, we report the screening and isolation of fungal species collected from the soil and mosses in the Schirmacher Hills, Dronning Maud Land, Antarctica, that produce L-Asparaginase free of glutaminase and urease. A total of 55 isolates were obtained from 33 environmental samples that were tested by conventional plate techniques using Phenol red and Bromothymol blue as indicators. Among the isolated fungi, 30 isolates showed L-Asparaginase free of glutaminase and urease. The L-Asparaginase producing strain Trichosporon asahii IBBLA1, which showed the highest zone index, was then optimized with a Taguchi design. Optimum enzyme activity of 20.57 U mL−1 was obtained at a temperature of 30 °C and pH of 7.0 after 60 hours. Our work suggests that isolation of fungi from extreme environments such as Antarctica may lead to an important advancement in therapeutic applications with fewer side effects. |
format |
Article in Journal/Newspaper |
author |
Ashok, Anup Doriya, Kruthi Rao, Jyothi Vithal Qureshi, Asif Tiwari, Anoop Kumar Kumar, Devarai Santhosh |
author_facet |
Ashok, Anup Doriya, Kruthi Rao, Jyothi Vithal Qureshi, Asif Tiwari, Anoop Kumar Kumar, Devarai Santhosh |
author_sort |
Ashok, Anup |
title |
Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss |
title_short |
Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss |
title_full |
Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss |
title_fullStr |
Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss |
title_full_unstemmed |
Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss |
title_sort |
microbes producing l-asparaginase free of glutaminase and urease isolated from extreme locations of antarctic soil and moss |
publisher |
Nature Publishing Group |
publishDate |
2019 |
url |
http://raiith.iith.ac.in/4810/ https://doi.org/10.1038/s41598-018-38094-1 http://raiith.iith.ac.in/4810/1/Scientific%20Reports_9_1_1-10_2019.pdf |
long_lat |
ENVELOPE(11.667,11.667,-70.750,-70.750) |
geographic |
Antarctic Dronning Maud Land Schirmacher Hills |
geographic_facet |
Antarctic Dronning Maud Land Schirmacher Hills |
genre |
Antarc* Antarctic Antarctica Dronning Maud Land |
genre_facet |
Antarc* Antarctic Antarctica Dronning Maud Land |
op_relation |
http://raiith.iith.ac.in/4810/1/Scientific%20Reports_9_1_1-10_2019.pdf Ashok, Anup and Doriya, Kruthi and Rao, Jyothi Vithal and Qureshi, Asif and Tiwari, Anoop Kumar and Kumar, Devarai Santhosh (2019) Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss. Scientific Reports, 9 (1). pp. 1-10. ISSN 2045-2322 |
op_doi |
https://doi.org/10.1038/s41598-018-38094-1 |
container_title |
Scientific Reports |
container_volume |
9 |
container_issue |
1 |
_version_ |
1766278592569802752 |