Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries
Nowadays, collagen is widely introduced into oral and topical relief cosmeceutical products and is applied in the pharmaceutical industry in the form of hydrogels and collagenated scaffolds. However, due to the source of collagen in the market, it is a big concern for the Muslim community in terms o...
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International Institute for Halal Research and Training (INHART), International Islamic University Malaysia (IIUM)
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ftiislamicuniv:oai:generic.eprints.org:92122 2023-05-15T15:34:29+02:00 Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries Salleh, Faqihah Amid, Azura Nordin, Noor Faizul Hadry 2021 application/pdf http://irep.iium.edu.my/92122/ http://irep.iium.edu.my/92122/2/92122%20Cloning,%20expression%20and%20purification%20of%20collagen-like%20protein.pdf en eng International Institute for Halal Research and Training (INHART), International Islamic University Malaysia (IIUM) http://irep.iium.edu.my/92122/2/92122%20Cloning,%20expression%20and%20purification%20of%20collagen-like%20protein.pdf Salleh, Faqihah and Amid, Azura and Nordin, Noor Faizul Hadry (2021) Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries. In: Virtual International Halal Science Conference 21: "Enhancing and Strengthening Halal Industry through Science", 13th-14 July 2021, Kuala Lumpur. Q Science (General) Conference or Workshop Item PeerReviewed 2021 ftiislamicuniv 2022-10-29T13:23:08Z Nowadays, collagen is widely introduced into oral and topical relief cosmeceutical products and is applied in the pharmaceutical industry in the form of hydrogels and collagenated scaffolds. However, due to the source of collagen in the market, it is a big concern for the Muslim community in terms of Halalan and Toyyiban since it is mainly extracted from mammals, especially porcine (Haram) and it raises health issues of animal-to-human disease transmission such as mad cow disease, swine, and avian flu. Collagen from bacteria would be the best alternative for halal and non-animal collagen. In this study, the bacterial collagen that produces collagen-like protein (CLP) of Methylobacteria sp. 4-46 was synthetically synthesized by Genscript. The CLPM4-46 in pUC57 was transformed into Escherichia coli DH5α and digested with BamHI/NdeI. The digested product (CLPM4-46, 1.04kbp) was successfully ligated with pCold II plasmid (4.39kbp) and transformed into E. coli BL21 (DE3). The ligated CLP gene was expressed using M9 minimal media. The protein expressed by the recombinant E. coli was measured with the Bradford Assay and SDS-PAGE where the size of the protein was 34.9k Da, and the CLP recombinant protein was purified using Fast Protein Liquid Chromatography and the collagen was measured using Enzyfluo™ Collagen Assay Conference Object Avian flu IIUM Repository (IRep - International Islamic University Malaysia) Haram ENVELOPE(6.445,6.445,62.599,62.599) |
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IIUM Repository (IRep - International Islamic University Malaysia) |
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Q Science (General) Salleh, Faqihah Amid, Azura Nordin, Noor Faizul Hadry Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries |
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Nowadays, collagen is widely introduced into oral and topical relief cosmeceutical products and is applied in the pharmaceutical industry in the form of hydrogels and collagenated scaffolds. However, due to the source of collagen in the market, it is a big concern for the Muslim community in terms of Halalan and Toyyiban since it is mainly extracted from mammals, especially porcine (Haram) and it raises health issues of animal-to-human disease transmission such as mad cow disease, swine, and avian flu. Collagen from bacteria would be the best alternative for halal and non-animal collagen. In this study, the bacterial collagen that produces collagen-like protein (CLP) of Methylobacteria sp. 4-46 was synthetically synthesized by Genscript. The CLPM4-46 in pUC57 was transformed into Escherichia coli DH5α and digested with BamHI/NdeI. The digested product (CLPM4-46, 1.04kbp) was successfully ligated with pCold II plasmid (4.39kbp) and transformed into E. coli BL21 (DE3). The ligated CLP gene was expressed using M9 minimal media. The protein expressed by the recombinant E. coli was measured with the Bradford Assay and SDS-PAGE where the size of the protein was 34.9k Da, and the CLP recombinant protein was purified using Fast Protein Liquid Chromatography and the collagen was measured using Enzyfluo™ Collagen Assay |
format |
Conference Object |
author |
Salleh, Faqihah Amid, Azura Nordin, Noor Faizul Hadry |
author_facet |
Salleh, Faqihah Amid, Azura Nordin, Noor Faizul Hadry |
author_sort |
Salleh, Faqihah |
title |
Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries |
title_short |
Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries |
title_full |
Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries |
title_fullStr |
Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries |
title_full_unstemmed |
Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries |
title_sort |
cloning, expression and purification of collagen-like protein from methylobacteria sp. 4-46 as alternative substance for collagen-based industries |
publisher |
International Institute for Halal Research and Training (INHART), International Islamic University Malaysia (IIUM) |
publishDate |
2021 |
url |
http://irep.iium.edu.my/92122/ http://irep.iium.edu.my/92122/2/92122%20Cloning,%20expression%20and%20purification%20of%20collagen-like%20protein.pdf |
long_lat |
ENVELOPE(6.445,6.445,62.599,62.599) |
geographic |
Haram |
geographic_facet |
Haram |
genre |
Avian flu |
genre_facet |
Avian flu |
op_relation |
http://irep.iium.edu.my/92122/2/92122%20Cloning,%20expression%20and%20purification%20of%20collagen-like%20protein.pdf Salleh, Faqihah and Amid, Azura and Nordin, Noor Faizul Hadry (2021) Cloning, expression and purification of collagen-like protein from Methylobacteria sp. 4-46 as alternative substance for collagen-based industries. In: Virtual International Halal Science Conference 21: "Enhancing and Strengthening Halal Industry through Science", 13th-14 July 2021, Kuala Lumpur. |
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