Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12
Leucosporidium antarcticum strain PI12 was isolated from Casey Station, Antarctica. It was shown to be protease-producer. Full-length cDNA of PI12 protease gene was amplified by rapid amplification of cDNA ends (RACE) strategy with an open reading frame (ORF) of 2892 bp that coded for 963 amino acid...
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ftiislamicuniv:oai:generic.eprints.org:53236 2023-05-15T13:57:42+02:00 Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12 Alias, Norsyuhada Raja Abd. Rahman, Raja Noor Zaliha 2014-02-10 application/pdf http://irep.iium.edu.my/53236/ http://irep.iium.edu.my/53236/1/3rd-BWC-Abstract-Book_53236_11216.pdf en eng http://irep.iium.edu.my/53236/1/3rd-BWC-Abstract-Book_53236_11216.pdf Alias, Norsyuhada and Raja Abd. Rahman, Raja Noor Zaliha (2014) Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12. In: 3rd Biotechnology World Congress, 10-12 February 2014, Dubai, UAE. (Unpublished) QR Microbiology Conference or Workshop Item PeerReviewed 2014 ftiislamicuniv 2022-10-29T13:05:59Z Leucosporidium antarcticum strain PI12 was isolated from Casey Station, Antarctica. It was shown to be protease-producer. Full-length cDNA of PI12 protease gene was amplified by rapid amplification of cDNA ends (RACE) strategy with an open reading frame (ORF) of 2892 bp that coded for 963 amino acids. Homology search by BLAST web server has showed the PI12 protease sequence shared a significant homology to the subtilisin protease family from fungus but no similarity with other psychrophilic protease. The protease sequence was deposited into the GenBank Database with the accession no. CAQ76821. The gene encoding mature PI12 protease was cloned into Pichia pastoris expression vector, pPIC9, and positioned under the induction of methanol-alcohol oxidase (AOX) promoter. The recombinant PI12 protease was efficiently secreted into the culture medium driven by the Saccharomyces cerevisiae α-factor signal sequence. The highest protease production was obtained from P. pastoris GS115 host (GpPro2) with 20.3 U/mL activity at 15ºC after 3 days of induction time. The expressed protein was detected by SDS-PAGE and activity staining with a molecular weight of 99.3 kDa. Conference Object Antarc* Antarctic Antarctica Leucosporidium antarcticum IIUM Repository (IRep - International Islamic University Malaysia) Antarctic Casey Station ENVELOPE(110.528,110.528,-66.282,-66.282) |
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IIUM Repository (IRep - International Islamic University Malaysia) |
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English |
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QR Microbiology |
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QR Microbiology Alias, Norsyuhada Raja Abd. Rahman, Raja Noor Zaliha Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12 |
topic_facet |
QR Microbiology |
description |
Leucosporidium antarcticum strain PI12 was isolated from Casey Station, Antarctica. It was shown to be protease-producer. Full-length cDNA of PI12 protease gene was amplified by rapid amplification of cDNA ends (RACE) strategy with an open reading frame (ORF) of 2892 bp that coded for 963 amino acids. Homology search by BLAST web server has showed the PI12 protease sequence shared a significant homology to the subtilisin protease family from fungus but no similarity with other psychrophilic protease. The protease sequence was deposited into the GenBank Database with the accession no. CAQ76821. The gene encoding mature PI12 protease was cloned into Pichia pastoris expression vector, pPIC9, and positioned under the induction of methanol-alcohol oxidase (AOX) promoter. The recombinant PI12 protease was efficiently secreted into the culture medium driven by the Saccharomyces cerevisiae α-factor signal sequence. The highest protease production was obtained from P. pastoris GS115 host (GpPro2) with 20.3 U/mL activity at 15ºC after 3 days of induction time. The expressed protein was detected by SDS-PAGE and activity staining with a molecular weight of 99.3 kDa. |
format |
Conference Object |
author |
Alias, Norsyuhada Raja Abd. Rahman, Raja Noor Zaliha |
author_facet |
Alias, Norsyuhada Raja Abd. Rahman, Raja Noor Zaliha |
author_sort |
Alias, Norsyuhada |
title |
Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12 |
title_short |
Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12 |
title_full |
Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12 |
title_fullStr |
Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12 |
title_full_unstemmed |
Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12 |
title_sort |
cold-adapted serine protease from antarctic yeast leucosporidium antarcticum strain pi12 |
publishDate |
2014 |
url |
http://irep.iium.edu.my/53236/ http://irep.iium.edu.my/53236/1/3rd-BWC-Abstract-Book_53236_11216.pdf |
long_lat |
ENVELOPE(110.528,110.528,-66.282,-66.282) |
geographic |
Antarctic Casey Station |
geographic_facet |
Antarctic Casey Station |
genre |
Antarc* Antarctic Antarctica Leucosporidium antarcticum |
genre_facet |
Antarc* Antarctic Antarctica Leucosporidium antarcticum |
op_relation |
http://irep.iium.edu.my/53236/1/3rd-BWC-Abstract-Book_53236_11216.pdf Alias, Norsyuhada and Raja Abd. Rahman, Raja Noor Zaliha (2014) Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12. In: 3rd Biotechnology World Congress, 10-12 February 2014, Dubai, UAE. (Unpublished) |
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1766265516175917056 |