The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region

• Main Author: Yusof, Faridah
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2022
• Subjects: T Technology (General); TP248.13 Biotechnology; Antarctic; The Antarctic; Antarc*
• Online Access: http://irep.iium.edu.my/102082/; http://irep.iium.edu.my/102082/1/Asyraf%20%5BIIUMEJ%5D%202447_%20Editor%20Decision%20-%20Accept%201818.12.2022.pdf

The growth conditions of bacteria producing cold-active protease isolated from the Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was selected for optimization via Response Surface Method (RSM). The parameters studied were incubation temperature (4 - 36 °C), pH media (4 – 10) and NaCl concentration (0 - 8 %). Based on the OFAT results, all eight strains showed the highest growth rate at 20 °C, pH 7 and 4% (w/v) NaCl. The assay showed that the crude enzyme extracted from strain SC8 exhibited significantly higher activity (0.20 U and 0.37 U) than the positive control (0.11 U and 0.31 U) at -20 °C and 20 °C. RSM suggested that the optimized setting for growth of SC8 were at 20.5 °C, pH 6.83 and 2.05% (w/v) of NaCl with the results of the bacterial growth rate value was 3.70 ± 0.06 x 106 cells/hr. Optimal growth conditions of SC8 from this study are useful for the large-scale production of cold-active protease in future.