The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region
The growth conditions of bacteria producing cold-active protease isolated from the Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was...
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ftiislamicuniv:oai:generic.eprints.org:102082 2023-05-15T13:57:16+02:00 The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region Yusof, Faridah 2022 application/pdf http://irep.iium.edu.my/102082/ http://irep.iium.edu.my/102082/1/Asyraf%20%5BIIUMEJ%5D%202447_%20Editor%20Decision%20-%20Accept%201818.12.2022.pdf en eng http://irep.iium.edu.my/102082/1/Asyraf%20%5BIIUMEJ%5D%202447_%20Editor%20Decision%20-%20Accept%201818.12.2022.pdf Yusof, Faridah (2022) The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region. iium engineering journal. (In Press) T Technology (General) TP248.13 Biotechnology Article PeerReviewed 2022 ftiislamicuniv 2023-02-09T08:20:09Z The growth conditions of bacteria producing cold-active protease isolated from the Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was selected for optimization via Response Surface Method (RSM). The parameters studied were incubation temperature (4 - 36 °C), pH media (4 – 10) and NaCl concentration (0 - 8 %). Based on the OFAT results, all eight strains showed the highest growth rate at 20 °C, pH 7 and 4% (w/v) NaCl. The assay showed that the crude enzyme extracted from strain SC8 exhibited significantly higher activity (0.20 U and 0.37 U) than the positive control (0.11 U and 0.31 U) at -20 °C and 20 °C. RSM suggested that the optimized setting for growth of SC8 were at 20.5 °C, pH 6.83 and 2.05% (w/v) of NaCl with the results of the bacterial growth rate value was 3.70 ± 0.06 x 106 cells/hr. Optimal growth conditions of SC8 from this study are useful for the large-scale production of cold-active protease in future. Article in Journal/Newspaper Antarc* Antarctic IIUM Repository (IRep - International Islamic University Malaysia) Antarctic The Antarctic |
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IIUM Repository (IRep - International Islamic University Malaysia) |
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ftiislamicuniv |
language |
English |
topic |
T Technology (General) TP248.13 Biotechnology |
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T Technology (General) TP248.13 Biotechnology Yusof, Faridah The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region |
topic_facet |
T Technology (General) TP248.13 Biotechnology |
description |
The growth conditions of bacteria producing cold-active protease isolated from the Antarctic sample were screened using one-factor-at-time (OFAT). Then, crude protease of the strain was extracted during late logarithmic phase for enzymatic assay. A strain that showed the highest enzyme activity was selected for optimization via Response Surface Method (RSM). The parameters studied were incubation temperature (4 - 36 °C), pH media (4 – 10) and NaCl concentration (0 - 8 %). Based on the OFAT results, all eight strains showed the highest growth rate at 20 °C, pH 7 and 4% (w/v) NaCl. The assay showed that the crude enzyme extracted from strain SC8 exhibited significantly higher activity (0.20 U and 0.37 U) than the positive control (0.11 U and 0.31 U) at -20 °C and 20 °C. RSM suggested that the optimized setting for growth of SC8 were at 20.5 °C, pH 6.83 and 2.05% (w/v) of NaCl with the results of the bacterial growth rate value was 3.70 ± 0.06 x 106 cells/hr. Optimal growth conditions of SC8 from this study are useful for the large-scale production of cold-active protease in future. |
format |
Article in Journal/Newspaper |
author |
Yusof, Faridah |
author_facet |
Yusof, Faridah |
author_sort |
Yusof, Faridah |
title |
The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region |
title_short |
The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region |
title_full |
The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region |
title_fullStr |
The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region |
title_full_unstemmed |
The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region |
title_sort |
optimization of growth condition of the bacteria producing cold-active proteolytic enzyme from the antarctic region |
publishDate |
2022 |
url |
http://irep.iium.edu.my/102082/ http://irep.iium.edu.my/102082/1/Asyraf%20%5BIIUMEJ%5D%202447_%20Editor%20Decision%20-%20Accept%201818.12.2022.pdf |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://irep.iium.edu.my/102082/1/Asyraf%20%5BIIUMEJ%5D%202447_%20Editor%20Decision%20-%20Accept%201818.12.2022.pdf Yusof, Faridah (2022) The optimization of growth condition of the bacteria producing Cold-Active Proteolytic Enzyme from the Antarctic region. iium engineering journal. (In Press) |
_version_ |
1766264865664532480 |