Morphology, phylogeny and PSP toxin composition of Alexandrium spp. isolated from Irish coastal waters
The dinoflagellate genus Alexandrium (Halim) has received considerable attention in recent years. In part, this has been due to recent taxonomic advances within the Dinophyceae. Many species from this genus however, produce paralytic shellfish poisoning (PSP) toxins that have had serious economic im...
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ftieo:oai:repositorio.ieo.es:10508/9926 2023-05-15T17:41:28+02:00 Morphology, phylogeny and PSP toxin composition of Alexandrium spp. isolated from Irish coastal waters Touzet, N. (Nicolás) Paz, B. (Beatriz) Riobó, P. (Pilar) Franco, J.M. (José Mariano) Raine, R. (Robin) Atlantic Ocean Eastern Atlantic Northeast Atlantic Irish Sea http://hdl.handle.net/10508/9926 eng eng Marine Institute Centro Oceanográfico de Vigo http://hdl.handle.net/10508/9926 Molluscan shellfish safety: Proceedings of the 5th International Conference on Molluscan Shellfish Safety. (ed.). Marine Institute. Galway (Irlanda). 2006. 508 pp: 248-252 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess CC-BY-NC-ND bookPart ftieo 2022-07-26T23:48:51Z The dinoflagellate genus Alexandrium (Halim) has received considerable attention in recent years. In part, this has been due to recent taxonomic advances within the Dinophyceae. Many species from this genus however, produce paralytic shellfish poisoning (PSP) toxins that have had serious economic impacts within the aquaculture industry worldwide. The occurrence of PSP toxins in shellfish from the south coast of Ireland has been linked to the suspected presence of an Alexandrium population. This study describes what is currently understood of the distribution of Alexandrium spp. around Ireland, in terms of both species diversity and toxicity. Alexandrium cysts and vegetative cells were collected from sediment and water column samples from Irish coastal waters. These were used to establish a suite of mono-specific cultures. Morphological identification of the vegetative cells using the fluorescent stain Calcofluor White was confirmed by partial sequencing of amplified LSU rDNA. Toxicity testing showed that of all the species isolated only Alexandrium minutum from the south coast produced PSP toxins. This species had both a toxin profile (GTX-2 and GTX-3) and a distribution which confirmed that it has been responsible for historical occurrences of contamination of shellfish with PSP toxins. Versión del editor Book Part Northeast Atlantic Instituto Español de Oceanografía: e-IEO |
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Instituto Español de Oceanografía: e-IEO |
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English |
description |
The dinoflagellate genus Alexandrium (Halim) has received considerable attention in recent years. In part, this has been due to recent taxonomic advances within the Dinophyceae. Many species from this genus however, produce paralytic shellfish poisoning (PSP) toxins that have had serious economic impacts within the aquaculture industry worldwide. The occurrence of PSP toxins in shellfish from the south coast of Ireland has been linked to the suspected presence of an Alexandrium population. This study describes what is currently understood of the distribution of Alexandrium spp. around Ireland, in terms of both species diversity and toxicity. Alexandrium cysts and vegetative cells were collected from sediment and water column samples from Irish coastal waters. These were used to establish a suite of mono-specific cultures. Morphological identification of the vegetative cells using the fluorescent stain Calcofluor White was confirmed by partial sequencing of amplified LSU rDNA. Toxicity testing showed that of all the species isolated only Alexandrium minutum from the south coast produced PSP toxins. This species had both a toxin profile (GTX-2 and GTX-3) and a distribution which confirmed that it has been responsible for historical occurrences of contamination of shellfish with PSP toxins. Versión del editor |
format |
Book Part |
author |
Touzet, N. (Nicolás) Paz, B. (Beatriz) Riobó, P. (Pilar) Franco, J.M. (José Mariano) Raine, R. (Robin) |
spellingShingle |
Touzet, N. (Nicolás) Paz, B. (Beatriz) Riobó, P. (Pilar) Franco, J.M. (José Mariano) Raine, R. (Robin) Morphology, phylogeny and PSP toxin composition of Alexandrium spp. isolated from Irish coastal waters |
author_facet |
Touzet, N. (Nicolás) Paz, B. (Beatriz) Riobó, P. (Pilar) Franco, J.M. (José Mariano) Raine, R. (Robin) |
author_sort |
Touzet, N. (Nicolás) |
title |
Morphology, phylogeny and PSP toxin composition of Alexandrium spp. isolated from Irish coastal waters |
title_short |
Morphology, phylogeny and PSP toxin composition of Alexandrium spp. isolated from Irish coastal waters |
title_full |
Morphology, phylogeny and PSP toxin composition of Alexandrium spp. isolated from Irish coastal waters |
title_fullStr |
Morphology, phylogeny and PSP toxin composition of Alexandrium spp. isolated from Irish coastal waters |
title_full_unstemmed |
Morphology, phylogeny and PSP toxin composition of Alexandrium spp. isolated from Irish coastal waters |
title_sort |
morphology, phylogeny and psp toxin composition of alexandrium spp. isolated from irish coastal waters |
publisher |
Marine Institute |
url |
http://hdl.handle.net/10508/9926 |
op_coverage |
Atlantic Ocean Eastern Atlantic Northeast Atlantic Irish Sea |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_relation |
http://hdl.handle.net/10508/9926 Molluscan shellfish safety: Proceedings of the 5th International Conference on Molluscan Shellfish Safety. (ed.). Marine Institute. Galway (Irlanda). 2006. 508 pp: 248-252 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766143053448347648 |