Characterization of Nontoxic and Toxin-Producing Strains of Alexandrium minutum (Dinophyceae) in Irish Coastal Waters
A comparative analysis of the morphology, toxin composition, and ribosomal DNA (rDNA) sequences was performed on a suite of clonal cultures of the potentially toxic dinoflagellate Alexandrium minutum Halim. These were established from resting cysts or vegetative cells isolated from sediment and wate...
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ftieo:oai:repositorio.ieo.es:10508/9915 2023-05-15T17:41:32+02:00 Characterization of Nontoxic and Toxin-Producing Strains of Alexandrium minutum (Dinophyceae) in Irish Coastal Waters Touzet, N. (Nicolás) Franco, J.M. (José Mariano) Raine, R. (Robin) Atlantic Ocean Eastern Atlantic Northeast Atlantic Irish Sea http://hdl.handle.net/10508/9915 https://doi.org/10.1128/AEM.02161-06 eng eng Centro Oceanográfico de Vigo http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1907102/ 0099-2240 http://hdl.handle.net/10508/9915 Applied and Environmental Microbiology, 73(10). 2007: 3333-3342 doi:10.1128/AEM.02161-06 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess CC-BY-NC-ND article ftieo https://doi.org/10.1128/AEM.02161-06 2022-07-26T23:48:51Z A comparative analysis of the morphology, toxin composition, and ribosomal DNA (rDNA) sequences was performed on a suite of clonal cultures of the potentially toxic dinoflagellate Alexandrium minutum Halim. These were established from resting cysts or vegetative cells isolated from sediment and water samples taken from the south and west coasts of Ireland. Results revealed that strains were indistinguishable, both morphologically and through the sequencing of the D1-D2 domain of the large subunit and the ITS1-5.8S-ITS2 regions of the rDNA. High-performance liquid chromatography fluorescence detection analysis, however, showed that only strains derived from retentive inlets on the southern Irish coast synthesized paralytic shellfish poisoning (PSP) toxins (GTX2 and GTX3), whereas all strains of A. minutum isolated from the west coast were nontoxic. Toxin analysis of net hauls, taken when A. minutum vegetative cells were in the water column, revealed no PSP toxins in samples from Killary Harbor (western coast), whereas GTX2 and GTX3 were detected in samples from Cork Harbor (southern coast). These results confirm the identity of A. minutum as the most probable causative organism for historical occurrences of contamination of shellfish with PSP toxins in Cork Harbor. Finally, random amplification of polymorphic DNA was carried out to determine the degree of polymorphism among strains. The analysis showed that all toxic strains from Cork Harbor clustered together and that a separate cluster grouped all nontoxic strains from the western coast. Versión del editor 3,8290 Article in Journal/Newspaper Northeast Atlantic Instituto Español de Oceanografía: e-IEO Applied and Environmental Microbiology 73 10 3333 3342 |
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Open Polar |
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Instituto Español de Oceanografía: e-IEO |
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ftieo |
language |
English |
description |
A comparative analysis of the morphology, toxin composition, and ribosomal DNA (rDNA) sequences was performed on a suite of clonal cultures of the potentially toxic dinoflagellate Alexandrium minutum Halim. These were established from resting cysts or vegetative cells isolated from sediment and water samples taken from the south and west coasts of Ireland. Results revealed that strains were indistinguishable, both morphologically and through the sequencing of the D1-D2 domain of the large subunit and the ITS1-5.8S-ITS2 regions of the rDNA. High-performance liquid chromatography fluorescence detection analysis, however, showed that only strains derived from retentive inlets on the southern Irish coast synthesized paralytic shellfish poisoning (PSP) toxins (GTX2 and GTX3), whereas all strains of A. minutum isolated from the west coast were nontoxic. Toxin analysis of net hauls, taken when A. minutum vegetative cells were in the water column, revealed no PSP toxins in samples from Killary Harbor (western coast), whereas GTX2 and GTX3 were detected in samples from Cork Harbor (southern coast). These results confirm the identity of A. minutum as the most probable causative organism for historical occurrences of contamination of shellfish with PSP toxins in Cork Harbor. Finally, random amplification of polymorphic DNA was carried out to determine the degree of polymorphism among strains. The analysis showed that all toxic strains from Cork Harbor clustered together and that a separate cluster grouped all nontoxic strains from the western coast. Versión del editor 3,8290 |
format |
Article in Journal/Newspaper |
author |
Touzet, N. (Nicolás) Franco, J.M. (José Mariano) Raine, R. (Robin) |
spellingShingle |
Touzet, N. (Nicolás) Franco, J.M. (José Mariano) Raine, R. (Robin) Characterization of Nontoxic and Toxin-Producing Strains of Alexandrium minutum (Dinophyceae) in Irish Coastal Waters |
author_facet |
Touzet, N. (Nicolás) Franco, J.M. (José Mariano) Raine, R. (Robin) |
author_sort |
Touzet, N. (Nicolás) |
title |
Characterization of Nontoxic and Toxin-Producing Strains of Alexandrium minutum (Dinophyceae) in Irish Coastal Waters |
title_short |
Characterization of Nontoxic and Toxin-Producing Strains of Alexandrium minutum (Dinophyceae) in Irish Coastal Waters |
title_full |
Characterization of Nontoxic and Toxin-Producing Strains of Alexandrium minutum (Dinophyceae) in Irish Coastal Waters |
title_fullStr |
Characterization of Nontoxic and Toxin-Producing Strains of Alexandrium minutum (Dinophyceae) in Irish Coastal Waters |
title_full_unstemmed |
Characterization of Nontoxic and Toxin-Producing Strains of Alexandrium minutum (Dinophyceae) in Irish Coastal Waters |
title_sort |
characterization of nontoxic and toxin-producing strains of alexandrium minutum (dinophyceae) in irish coastal waters |
publisher |
Centro Oceanográfico de Vigo |
url |
http://hdl.handle.net/10508/9915 https://doi.org/10.1128/AEM.02161-06 |
op_coverage |
Atlantic Ocean Eastern Atlantic Northeast Atlantic Irish Sea |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1907102/ 0099-2240 http://hdl.handle.net/10508/9915 Applied and Environmental Microbiology, 73(10). 2007: 3333-3342 doi:10.1128/AEM.02161-06 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1128/AEM.02161-06 |
container_title |
Applied and Environmental Microbiology |
container_volume |
73 |
container_issue |
10 |
container_start_page |
3333 |
op_container_end_page |
3342 |
_version_ |
1766143146341695488 |