Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters
The diversity of Alexandrium spp. in Irish coastal waters was investigated through the morphological examination of resting cysts and vegetative cells, the determination of PSP toxin and spirolide profiles and the sequence analysis of rDNA genes. Six morphospecies were characterised: A. tamarense, A...
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ftieo:oai:repositorio.ieo.es:10508/9907 2023-05-15T17:41:37+02:00 Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters Touzet, N. (Nicolás) Franco, J.M. (José Mariano) Raine, R. (Robin) Atlantic Ocean Eastern Atlantic Northeast Atlantic Irish Sea http://hdl.handle.net/10508/9907 https://doi.org/10.1016/j.hal.2008.04.001 eng eng Centro Oceanográfico de Vigo http://www.sciencedirect.com/science/article/pii/S1568988308000292 1568-9883 http://hdl.handle.net/10508/9907 Harmful Algae, 7(6). 2008: 782-797 doi:10.1016/j.hal.2008.04.001 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess CC-BY-NC-ND Alexandrium HAB PSP toxins rDNA Resting cysts Spirolides article ftieo https://doi.org/10.1016/j.hal.2008.04.001 2022-07-26T23:48:51Z The diversity of Alexandrium spp. in Irish coastal waters was investigated through the morphological examination of resting cysts and vegetative cells, the determination of PSP toxin and spirolide profiles and the sequence analysis of rDNA genes. Six morphospecies were characterised: A. tamarense, A. minutum, A. ostenfeldii, A. peruvianum, A. tamutum and A. andersoni. Both PSP toxin producing and non-toxic strains of A. tamarense and A. minutum were observed. The average toxicities of toxic strains for both cultured species were respectively 11.3 (8.6 S.D.) and 2.3 (0.5 S.D.) pg STX equiv. cell−1. Alexandrium ostenfeldii and A. peruvianum did not synthesise PSP toxins but HPLC–MS analysis of two strains showed distinct spirolide profiles. A cyst-derived culture of A. peruvianum from Lough Swilly mainly produced spirolides 13 desmethyl-C and 13 desmethyl-D whereas one of A. ostenfeldii, from Bantry Bay, produced spirolides C and D. Species identification was confirmed through the analyses of SSU, ITS1-5.8S-ITS2 and LSU rDNA genes. Some nucleotide variability was observed among clones of toxic strains of A. tamarense, which all clustered within the North American clade. However, rDNA sequencing did not allow discrimination between the toxic and non-toxic forms of A. minutum. Phylogenetic analysis also permitted the differentiation of A. ostenfeldii from A. peruvianum. Resting cysts of PSP toxin producing Alexandrium species were found in Cork Harbour and Belfast Lough, locations where shellfish contamination events have occurred in the past, highlighting the potential for the initiation of harmful blooms from cyst beds. The finding of supposedly non-toxic and biotoxin-producing Alexandrium species near aquaculture production sites will necessitate the use of reliable discriminative methods in phytoplankton monitoring. Versión del editor 3,0830 Article in Journal/Newspaper Northeast Atlantic Instituto Español de Oceanografía: e-IEO Harmful Algae 7 6 782 797 |
institution |
Open Polar |
collection |
Instituto Español de Oceanografía: e-IEO |
op_collection_id |
ftieo |
language |
English |
topic |
Alexandrium HAB PSP toxins rDNA Resting cysts Spirolides |
spellingShingle |
Alexandrium HAB PSP toxins rDNA Resting cysts Spirolides Touzet, N. (Nicolás) Franco, J.M. (José Mariano) Raine, R. (Robin) Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
topic_facet |
Alexandrium HAB PSP toxins rDNA Resting cysts Spirolides |
description |
The diversity of Alexandrium spp. in Irish coastal waters was investigated through the morphological examination of resting cysts and vegetative cells, the determination of PSP toxin and spirolide profiles and the sequence analysis of rDNA genes. Six morphospecies were characterised: A. tamarense, A. minutum, A. ostenfeldii, A. peruvianum, A. tamutum and A. andersoni. Both PSP toxin producing and non-toxic strains of A. tamarense and A. minutum were observed. The average toxicities of toxic strains for both cultured species were respectively 11.3 (8.6 S.D.) and 2.3 (0.5 S.D.) pg STX equiv. cell−1. Alexandrium ostenfeldii and A. peruvianum did not synthesise PSP toxins but HPLC–MS analysis of two strains showed distinct spirolide profiles. A cyst-derived culture of A. peruvianum from Lough Swilly mainly produced spirolides 13 desmethyl-C and 13 desmethyl-D whereas one of A. ostenfeldii, from Bantry Bay, produced spirolides C and D. Species identification was confirmed through the analyses of SSU, ITS1-5.8S-ITS2 and LSU rDNA genes. Some nucleotide variability was observed among clones of toxic strains of A. tamarense, which all clustered within the North American clade. However, rDNA sequencing did not allow discrimination between the toxic and non-toxic forms of A. minutum. Phylogenetic analysis also permitted the differentiation of A. ostenfeldii from A. peruvianum. Resting cysts of PSP toxin producing Alexandrium species were found in Cork Harbour and Belfast Lough, locations where shellfish contamination events have occurred in the past, highlighting the potential for the initiation of harmful blooms from cyst beds. The finding of supposedly non-toxic and biotoxin-producing Alexandrium species near aquaculture production sites will necessitate the use of reliable discriminative methods in phytoplankton monitoring. Versión del editor 3,0830 |
format |
Article in Journal/Newspaper |
author |
Touzet, N. (Nicolás) Franco, J.M. (José Mariano) Raine, R. (Robin) |
author_facet |
Touzet, N. (Nicolás) Franco, J.M. (José Mariano) Raine, R. (Robin) |
author_sort |
Touzet, N. (Nicolás) |
title |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_short |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_full |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_fullStr |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_full_unstemmed |
Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters |
title_sort |
morphogenetic diversity and biotoxin composition of alexandrium (dinophyceae) in irish coastal waters |
publisher |
Centro Oceanográfico de Vigo |
url |
http://hdl.handle.net/10508/9907 https://doi.org/10.1016/j.hal.2008.04.001 |
op_coverage |
Atlantic Ocean Eastern Atlantic Northeast Atlantic Irish Sea |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1568988308000292 1568-9883 http://hdl.handle.net/10508/9907 Harmful Algae, 7(6). 2008: 782-797 doi:10.1016/j.hal.2008.04.001 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.hal.2008.04.001 |
container_title |
Harmful Algae |
container_volume |
7 |
container_issue |
6 |
container_start_page |
782 |
op_container_end_page |
797 |
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1766143278214807552 |