Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of d- and l-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35°N to 5°S). Fluorescence in situ hybridization (FISH)...
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Centro Oceanográfico de A Coruña
2008
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Online Access: | http://hdl.handle.net/10508/7641 https://doi.org/10.1111/j.1462-2920.2008.01627.x |
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ftieo:oai:repositorio.ieo.es:10508/7641 2023-05-15T17:36:21+02:00 Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic Varela, M.M. (Marta María) Van-Aken, H.M. (Hendrik M.) Herndl, G.J. Atlantic Ocean Central Atlantic Tropical Atlantic 2008 http://hdl.handle.net/10508/7641 https://doi.org/10.1111/j.1462-2920.2008.01627.x unknown Centro Oceanográfico de A Coruña https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2008.01627.x http://hdl.handle.net/10508/7641 Environmental Microbiology, 10. 2008: 1903-1911 1462-2920 doi:10.1111/j.1462-2920.2008.01627.x Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access CC-BY-NC-ND research article 2008 ftieo https://doi.org/10.1111/j.1462-2920.2008.01627.x 2023-03-08T00:50:48Z The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of d- and l-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35°N to 5°S). Fluorescence in situ hybridization (FISH) revealed that the overall abundance of SAR202 was ≤ 1 × 103 cells ml−1 in subsurface waters (100 m layer), increasing in the mesopelagic zone to 3 × 103 cells ml−1 and remaining fairly constant down to 4000 m depth. Overall, the percentage of total picoplankton identified as SAR202 increased from < 1% in subsurface waters to 10–20% in the bathypelagic waters. On average, members of the SAR202 cluster accounted for about 30% of the Bacteria in the bathypelagic waters, whereas in the mesopelagic and subsurface waters, SAR202 cells contributed < 5% to total bacterial abundance. The ratio of d-Asp : l-Asp uptake by the bulk picoplankton community increased from the subsurface layer (d-Asp : l-Asp uptake ratio ≈ 0.03) to the deeper layers reaching a ratio of ∼1 at 4000 m depth. Combining FISH with microautoradiography to determine the proportion of SAR202 cells taking up d-Asp versus l-Asp, we found that ≈ 30% of the SAR202 cells were taking up l-Asp throughout the water column while d-Asp was essentially not taken up by SAR202. This d-Asp : l-Asp uptake pattern of SAR202 cells is in contrast to that of the bulk bacterial and crenarchaeal community in the bathypelagic ocean, both sustaining a higher fraction of d-Asp-positive cells than l-Asp-positive cells. Thus, although the Chloroflexi-type SAR202 constitutes a major bathypelagic bacterial cluster, it does not contribute to the large fraction of d-Asp utilizing prokaryotic community in the meso- and bathypelagic waters of the North Atlantic, but rather utilizes preferentially l-amino acids. 6 Article in Journal/Newspaper North Atlantic Instituto Español de Oceanografía: e-IEO Environmental Microbiology 10 7 1903 1911 |
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Instituto Español de Oceanografía: e-IEO |
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language |
unknown |
description |
The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of d- and l-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35°N to 5°S). Fluorescence in situ hybridization (FISH) revealed that the overall abundance of SAR202 was ≤ 1 × 103 cells ml−1 in subsurface waters (100 m layer), increasing in the mesopelagic zone to 3 × 103 cells ml−1 and remaining fairly constant down to 4000 m depth. Overall, the percentage of total picoplankton identified as SAR202 increased from < 1% in subsurface waters to 10–20% in the bathypelagic waters. On average, members of the SAR202 cluster accounted for about 30% of the Bacteria in the bathypelagic waters, whereas in the mesopelagic and subsurface waters, SAR202 cells contributed < 5% to total bacterial abundance. The ratio of d-Asp : l-Asp uptake by the bulk picoplankton community increased from the subsurface layer (d-Asp : l-Asp uptake ratio ≈ 0.03) to the deeper layers reaching a ratio of ∼1 at 4000 m depth. Combining FISH with microautoradiography to determine the proportion of SAR202 cells taking up d-Asp versus l-Asp, we found that ≈ 30% of the SAR202 cells were taking up l-Asp throughout the water column while d-Asp was essentially not taken up by SAR202. This d-Asp : l-Asp uptake pattern of SAR202 cells is in contrast to that of the bulk bacterial and crenarchaeal community in the bathypelagic ocean, both sustaining a higher fraction of d-Asp-positive cells than l-Asp-positive cells. Thus, although the Chloroflexi-type SAR202 constitutes a major bathypelagic bacterial cluster, it does not contribute to the large fraction of d-Asp utilizing prokaryotic community in the meso- and bathypelagic waters of the North Atlantic, but rather utilizes preferentially l-amino acids. 6 |
format |
Article in Journal/Newspaper |
author |
Varela, M.M. (Marta María) Van-Aken, H.M. (Hendrik M.) Herndl, G.J. |
spellingShingle |
Varela, M.M. (Marta María) Van-Aken, H.M. (Hendrik M.) Herndl, G.J. Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic |
author_facet |
Varela, M.M. (Marta María) Van-Aken, H.M. (Hendrik M.) Herndl, G.J. |
author_sort |
Varela, M.M. (Marta María) |
title |
Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic |
title_short |
Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic |
title_full |
Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic |
title_fullStr |
Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic |
title_full_unstemmed |
Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic |
title_sort |
abundance and activity of chloroflexi-type sar202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical atlantic |
publisher |
Centro Oceanográfico de A Coruña |
publishDate |
2008 |
url |
http://hdl.handle.net/10508/7641 https://doi.org/10.1111/j.1462-2920.2008.01627.x |
op_coverage |
Atlantic Ocean Central Atlantic Tropical Atlantic |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2008.01627.x http://hdl.handle.net/10508/7641 Environmental Microbiology, 10. 2008: 1903-1911 1462-2920 doi:10.1111/j.1462-2920.2008.01627.x |
op_rights |
Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1111/j.1462-2920.2008.01627.x |
container_title |
Environmental Microbiology |
container_volume |
10 |
container_issue |
7 |
container_start_page |
1903 |
op_container_end_page |
1911 |
_version_ |
1766135805095444480 |