Cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures
The percentage of cells dividing in a specific tissue of individual larvae can be estimated by analyzing DNA per cell by flow cytometry. An experimental test was carried out with cod (Gadus morhua) larvae, with brain as the target tissue, to validate this technique as an appropriate growth index for...
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Centro Oceanográfico de Gijón
2007
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Online Access: | http://hdl.handle.net/10508/7567 https://doi.org/10.3989/scimar.2007.71n3485 |
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ftieo:oai:repositorio.ieo.es:10508/7567 2023-05-15T16:19:14+02:00 Cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures González-Quirós, R. (Rafael) Munuera-Fernández, I. (Itziar) Flokvord, A. 2007 http://hdl.handle.net/10508/7567 https://doi.org/10.3989/scimar.2007.71n3485 unknown Centro Oceanográfico de Gijón https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/53 0214-8358 http://hdl.handle.net/10508/7567 Scientia Marina, 71. 2007: 485-497 doi:10.3989/scimar.2007.71n3485 Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access research article 2007 ftieo https://doi.org/10.3989/scimar.2007.71n3485 2023-03-15T00:56:10Z The percentage of cells dividing in a specific tissue of individual larvae can be estimated by analyzing DNA per cell by flow cytometry. An experimental test was carried out with cod (Gadus morhua) larvae, with brain as the target tissue, to validate this technique as an appropriate growth index for larval fish. Standard length (SL), myotome height, and %S-phase (% of cells in the S-phase of the cell-division cycle) variability were analyzed, with temperature (6 and 10°C), food level (high- and no-food) and larval developmental stage (first feeding, pre-metamorphosis and post-metamorphosis) as independent factors. Cod larvae grew faster (in SL) and presented a higher %S-phase under high-food conditions. Larval SL increased with temperature in rearing and experimental tanks. However, there was a significant interaction between temperature and food in the %S-phase. There were no significant differences in the %S-phase between 6 and 10°C at high-food levels. We suggest that this result is a consequence of temperature-dependency of the duration of the cell cycle. In the absence of food, larvae at 10ºC had a lower %S-phase than larvae at 6°C, which may be related to increased metabolic costs with increasing temperature. Considering the effect of temperature, the mean % S-phase explained 74% of the variability in the estimated standard growth rate. 1 Article in Journal/Newspaper Gadus morhua Instituto Español de Oceanografía: e-IEO Scientia Marina 71 3 485 497 |
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Instituto Español de Oceanografía: e-IEO |
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The percentage of cells dividing in a specific tissue of individual larvae can be estimated by analyzing DNA per cell by flow cytometry. An experimental test was carried out with cod (Gadus morhua) larvae, with brain as the target tissue, to validate this technique as an appropriate growth index for larval fish. Standard length (SL), myotome height, and %S-phase (% of cells in the S-phase of the cell-division cycle) variability were analyzed, with temperature (6 and 10°C), food level (high- and no-food) and larval developmental stage (first feeding, pre-metamorphosis and post-metamorphosis) as independent factors. Cod larvae grew faster (in SL) and presented a higher %S-phase under high-food conditions. Larval SL increased with temperature in rearing and experimental tanks. However, there was a significant interaction between temperature and food in the %S-phase. There were no significant differences in the %S-phase between 6 and 10°C at high-food levels. We suggest that this result is a consequence of temperature-dependency of the duration of the cell cycle. In the absence of food, larvae at 10ºC had a lower %S-phase than larvae at 6°C, which may be related to increased metabolic costs with increasing temperature. Considering the effect of temperature, the mean % S-phase explained 74% of the variability in the estimated standard growth rate. 1 |
format |
Article in Journal/Newspaper |
author |
González-Quirós, R. (Rafael) Munuera-Fernández, I. (Itziar) Flokvord, A. |
spellingShingle |
González-Quirós, R. (Rafael) Munuera-Fernández, I. (Itziar) Flokvord, A. Cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
author_facet |
González-Quirós, R. (Rafael) Munuera-Fernández, I. (Itziar) Flokvord, A. |
author_sort |
González-Quirós, R. (Rafael) |
title |
Cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_short |
Cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_full |
Cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_fullStr |
Cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_full_unstemmed |
Cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_sort |
cell cycle analyisis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
publisher |
Centro Oceanográfico de Gijón |
publishDate |
2007 |
url |
http://hdl.handle.net/10508/7567 https://doi.org/10.3989/scimar.2007.71n3485 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_relation |
https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/53 0214-8358 http://hdl.handle.net/10508/7567 Scientia Marina, 71. 2007: 485-497 doi:10.3989/scimar.2007.71n3485 |
op_rights |
Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access |
op_doi |
https://doi.org/10.3989/scimar.2007.71n3485 |
container_title |
Scientia Marina |
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71 |
container_issue |
3 |
container_start_page |
485 |
op_container_end_page |
497 |
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1766005591472340992 |