Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.)
An enzymoimmunoassay (EIA) kit for plasma melatonin (MLT) measurements was employed in tench (Tinca tinca) and in turbot (Scophtalmus maximus). Tench and turbot plasma samples were purified with a C18 reversed phase extraction columns because this kit is designed for human serum measurements. The lo...
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Centro Oceanográfico de Vigo
1999
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ftieo:oai:repositorio.ieo.es:10508/6915 2023-05-15T18:15:49+02:00 Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) Rebollar, P.J. Ubilla, E. Peleteiro-Alonso, J.B. (José Benito) Alvariño, M. 1999 http://hdl.handle.net/10508/6915 unknown Centro Oceanográfico de Vigo http://hdl.handle.net/10508/6915 Journal of Physiology and Biochemistry, 55(4). 1999: 341-348 Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access research article 1999 ftieo 2023-03-22T00:49:51Z An enzymoimmunoassay (EIA) kit for plasma melatonin (MLT) measurements was employed in tench (Tinca tinca) and in turbot (Scophtalmus maximus). Tench and turbot plasma samples were purified with a C18 reversed phase extraction columns because this kit is designed for human serum measurements. The lowest detection limit of the technique was 11.48 pg/well with a sensitivity at 50% binding of 100 pg/well. Intra-assay and inter-assay CV (%) were always less than 5% (n=8), and 9% (n=6) in tench plasma samples, and less than 5% (n=8) and 13% (n=5) in turbot plasma samples, respectively. Correlation coefficients between EIA and RIA measurements in tench and turbot plasma samples were 0.93 and 0.89 (p<0.001) respectively. Diurnal and nocturnal plasma melatonin mean levels were 14.7+/-2.1 pg/ml and 87.4+/-11 pg/ml in tench (n=15), and 3.5+/-0.4 pg/ml and 28.1+/-2.1 pg/ml in turbot (n=15). These species showed a melatonin circadian rhythm as in other animals studied. The results suggest that the commercial kit used in this experiment could be a suitable and alternative method to RIA for plasma MLT determinations in tench and turbot although it is necessary to increase volumes (1ml) and concentrate daytime samples. 1 Article in Journal/Newspaper Scophthalmus maximus Turbot Instituto Español de Oceanografía: e-IEO Eia ENVELOPE(7.755,7.755,63.024,63.024) |
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Open Polar |
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Instituto Español de Oceanografía: e-IEO |
op_collection_id |
ftieo |
language |
unknown |
description |
An enzymoimmunoassay (EIA) kit for plasma melatonin (MLT) measurements was employed in tench (Tinca tinca) and in turbot (Scophtalmus maximus). Tench and turbot plasma samples were purified with a C18 reversed phase extraction columns because this kit is designed for human serum measurements. The lowest detection limit of the technique was 11.48 pg/well with a sensitivity at 50% binding of 100 pg/well. Intra-assay and inter-assay CV (%) were always less than 5% (n=8), and 9% (n=6) in tench plasma samples, and less than 5% (n=8) and 13% (n=5) in turbot plasma samples, respectively. Correlation coefficients between EIA and RIA measurements in tench and turbot plasma samples were 0.93 and 0.89 (p<0.001) respectively. Diurnal and nocturnal plasma melatonin mean levels were 14.7+/-2.1 pg/ml and 87.4+/-11 pg/ml in tench (n=15), and 3.5+/-0.4 pg/ml and 28.1+/-2.1 pg/ml in turbot (n=15). These species showed a melatonin circadian rhythm as in other animals studied. The results suggest that the commercial kit used in this experiment could be a suitable and alternative method to RIA for plasma MLT determinations in tench and turbot although it is necessary to increase volumes (1ml) and concentrate daytime samples. 1 |
format |
Article in Journal/Newspaper |
author |
Rebollar, P.J. Ubilla, E. Peleteiro-Alonso, J.B. (José Benito) Alvariño, M. |
spellingShingle |
Rebollar, P.J. Ubilla, E. Peleteiro-Alonso, J.B. (José Benito) Alvariño, M. Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
author_facet |
Rebollar, P.J. Ubilla, E. Peleteiro-Alonso, J.B. (José Benito) Alvariño, M. |
author_sort |
Rebollar, P.J. |
title |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_short |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_full |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_fullStr |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_full_unstemmed |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_sort |
determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (eia) in turbot (scophthalmus maximus l.) and tench (tinca tinca l.) |
publisher |
Centro Oceanográfico de Vigo |
publishDate |
1999 |
url |
http://hdl.handle.net/10508/6915 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://hdl.handle.net/10508/6915 Journal of Physiology and Biochemistry, 55(4). 1999: 341-348 |
op_rights |
Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access |
_version_ |
1766189048612782080 |