A device for assesing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (ISMI)
Research article Microbes in the dark ocean are exposed to hydrostatic pressure increasing with depth. Activity rate measurements and biomass production of dark ocean microbes are, however, almost exclusively performed under atmospheric pressure conditions due to technical constraints of sampling eq...
Published in: | Limnology and Oceanography: Methods |
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Centro Oceanográfico de A Coruña
2022
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Online Access: | http://hdl.handle.net/10508/16472 https://doi.org/10.1002/lom3.10528 |
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ftieo:oai:repositorio.ieo.es:10508/16472 2023-05-15T17:34:59+02:00 A device for assesing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (ISMI) Amano, C. (Chie) Reinthaler, T. (Thomas) Sintes, E. (Eva) Varela, M.M. (Marta María) Stefanschitz, J. (Julia) Kaneko, S. (Sho) Año 2018 Océano Pacífico Pacific Ocean Océan Pacifique 2022-12-14 http://hdl.handle.net/10508/16472 https://doi.org/10.1002/lom3.10528 eng eng Centro Oceanográfico de A Coruña MerMet 17-97 and Radiales Profundos, JSPS KAKENHI Gran 23651004, FWF project I486-B09 and Z194 and EC/FP7/2007-2013, MEDEA project (ERC/268595); FWF project P27696-B22 and P23221-B11, IEO-GAIN and GRC grant (INGO7A 2018/2) y JSPS H26-168 and Madame Curie grant nº 701324 1541-5856 http://hdl.handle.net/10508/16472 doi:10.1002/lom3.10528 Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access CC-BY-NC-ND Microbial activity Incubator device Hydrostatic pressure Activity rate measurement of microbes Biomass production Dark ocean limnology ecology oceanography microorganisms research article VoR 2022 ftieo https://doi.org/10.1002/lom3.10528 2023-01-04T00:47:23Z Research article Microbes in the dark ocean are exposed to hydrostatic pressure increasing with depth. Activity rate measurements and biomass production of dark ocean microbes are, however, almost exclusively performed under atmospheric pressure conditions due to technical constraints of sampling equipment maintaining in situ pressure conditions. To evaluate the microbial activity under in situ hydrostatic pressure, we designed and thoroughly tested an in situ microbial incubator (ISMI). The ISMI allows autonomously collecting and incubating seawater at depth, injection of substrate and fixation of the samples after a preprogramed incubation time. The performance of the ISMI was tested in a high-pressure tank and in several field campaigns under ambient hydrostatic pressure by measuring prokaryotic bulk 3H-leucine incorporation rates. Overall, prokaryotic leucine incorporation rates were lower at in situ pressure conditions than under to depressurized conditions reaching only about 50% of the heterotrophic microbial activity measured under depressurized conditions in bathypelagic waters in the North Atlantic Ocean off the northwestern Iberian Peninsula. Our results show that the ISMI is a valuable tool to reliably determine the metabolic activity of deep-sea microbes at in situ hydrostatic pressure conditions. Hence, we advocate that deep-sea biogeochemical and microbial rate measurements should be performed under in situ pressure conditions to obtain a more realistic view on deep-sea biotic processes. IEO-CSIC, FWF, KAKENHI, ERC and GAIN Article in Journal/Newspaper North Atlantic Instituto Español de Oceanografía: e-IEO Pacific Limnology and Oceanography: Methods |
institution |
Open Polar |
collection |
Instituto Español de Oceanografía: e-IEO |
op_collection_id |
ftieo |
language |
English |
topic |
Microbial activity Incubator device Hydrostatic pressure Activity rate measurement of microbes Biomass production Dark ocean limnology ecology oceanography microorganisms |
spellingShingle |
Microbial activity Incubator device Hydrostatic pressure Activity rate measurement of microbes Biomass production Dark ocean limnology ecology oceanography microorganisms Amano, C. (Chie) Reinthaler, T. (Thomas) Sintes, E. (Eva) Varela, M.M. (Marta María) Stefanschitz, J. (Julia) Kaneko, S. (Sho) A device for assesing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (ISMI) |
topic_facet |
Microbial activity Incubator device Hydrostatic pressure Activity rate measurement of microbes Biomass production Dark ocean limnology ecology oceanography microorganisms |
description |
Research article Microbes in the dark ocean are exposed to hydrostatic pressure increasing with depth. Activity rate measurements and biomass production of dark ocean microbes are, however, almost exclusively performed under atmospheric pressure conditions due to technical constraints of sampling equipment maintaining in situ pressure conditions. To evaluate the microbial activity under in situ hydrostatic pressure, we designed and thoroughly tested an in situ microbial incubator (ISMI). The ISMI allows autonomously collecting and incubating seawater at depth, injection of substrate and fixation of the samples after a preprogramed incubation time. The performance of the ISMI was tested in a high-pressure tank and in several field campaigns under ambient hydrostatic pressure by measuring prokaryotic bulk 3H-leucine incorporation rates. Overall, prokaryotic leucine incorporation rates were lower at in situ pressure conditions than under to depressurized conditions reaching only about 50% of the heterotrophic microbial activity measured under depressurized conditions in bathypelagic waters in the North Atlantic Ocean off the northwestern Iberian Peninsula. Our results show that the ISMI is a valuable tool to reliably determine the metabolic activity of deep-sea microbes at in situ hydrostatic pressure conditions. Hence, we advocate that deep-sea biogeochemical and microbial rate measurements should be performed under in situ pressure conditions to obtain a more realistic view on deep-sea biotic processes. IEO-CSIC, FWF, KAKENHI, ERC and GAIN |
format |
Article in Journal/Newspaper |
author |
Amano, C. (Chie) Reinthaler, T. (Thomas) Sintes, E. (Eva) Varela, M.M. (Marta María) Stefanschitz, J. (Julia) Kaneko, S. (Sho) |
author_facet |
Amano, C. (Chie) Reinthaler, T. (Thomas) Sintes, E. (Eva) Varela, M.M. (Marta María) Stefanschitz, J. (Julia) Kaneko, S. (Sho) |
author_sort |
Amano, C. (Chie) |
title |
A device for assesing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (ISMI) |
title_short |
A device for assesing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (ISMI) |
title_full |
A device for assesing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (ISMI) |
title_fullStr |
A device for assesing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (ISMI) |
title_full_unstemmed |
A device for assesing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator (ISMI) |
title_sort |
device for assesing microbial activity under ambient hydrostatic pressure: the in situ microbial incubator (ismi) |
publisher |
Centro Oceanográfico de A Coruña |
publishDate |
2022 |
url |
http://hdl.handle.net/10508/16472 https://doi.org/10.1002/lom3.10528 |
op_coverage |
Año 2018 Océano Pacífico Pacific Ocean Océan Pacifique |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
MerMet 17-97 and Radiales Profundos, JSPS KAKENHI Gran 23651004, FWF project I486-B09 and Z194 and EC/FP7/2007-2013, MEDEA project (ERC/268595); FWF project P27696-B22 and P23221-B11, IEO-GAIN and GRC grant (INGO7A 2018/2) y JSPS H26-168 and Madame Curie grant nº 701324 1541-5856 http://hdl.handle.net/10508/16472 doi:10.1002/lom3.10528 |
op_rights |
Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1002/lom3.10528 |
container_title |
Limnology and Oceanography: Methods |
_version_ |
1766133998531117056 |