Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean
Diazotrophy-related studies in the North Atlantic have largely focused on its western tropical area, leaving the subtropics and the east undersampled. We studied the longitudinal distribution of Trichodesmium, UCYN-A, UCYN-B, the putative Gammaproteobacterium g-24774A11 and Richelia (Het1) along 24....
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Centro Oceanográfico de A Coruña
2016
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Online Access: | http://hdl.handle.net/10508/10151 https://doi.org/10.1093/plankt/fbv121 |
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ftieo:oai:repositorio.ieo.es:10508/10151 2023-05-15T17:26:25+02:00 Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean Benavides, M. (Mar) Moisander, P.H. Daley, M.C. Bode, A. (Antonio) Arístegui, J. (Javier) Atlantic Ocean North Atlantic 2016-01-18 http://hdl.handle.net/10508/10151 https://doi.org/10.1093/plankt/fbv121 eng eng Centro Oceanográfico de A Coruña http://plankt.oxfordjournals.org/content/early/2016/01/18/plankt.fbv121.abstract?sid=16a196d0-45c3-42dc-b58f-d49abb072dd2 Consolider-Malaspina (CSD2008-00077), CAIBEX (CTM2007-66408- CO2-02), HOTMIX (CTM2011-30010-CO2-01) 0142-7873 http://hdl.handle.net/10508/10151 Journal of Plankton Research, 0. 2016: 1-11 doi:10.1093/plankt/fbv121 Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess CC-BY-NC-ND nifH qPCR N2 fixation upwelling North Atlantic Subtropical Gyre article 2016 ftieo https://doi.org/10.1093/plankt/fbv121 2022-08-30T23:47:24Z Diazotrophy-related studies in the North Atlantic have largely focused on its western tropical area, leaving the subtropics and the east undersampled. We studied the longitudinal distribution of Trichodesmium, UCYN-A, UCYN-B, the putative Gammaproteobacterium g-24774A11 and Richelia (Het1) along 24.58N, using quantitative polymerase chain reaction on different size fractions (10, 10–3 and 3–0.2 mm) and additional filament counts for Trichodesmium. Trichodesmium was the most abundant phylotype, followed by UCYN-A, g-24774A11 and Het1, with maximum abundances of 8.8 ! 105, 2.0 ! 105, 3.3 ! 103 and 3.4 ! 102 nifH copies L21, respectively, whereas UCYN-B was mostly undetected. A clear shift in the diazotroph community was observed at !308W, coinciding with the transition between the North Atlantic Subtropical Gyre boundary and inner core. This transition zone divided the transect into an eastern half dominated by UCYN-A and western half dominated by Trichodesmium and g-24774A11. g-24774A11 was only detected in the 10–3 mm fraction, suggesting their association with larger microbes or aggregates. Our results indicate that typical size fractionation by 10 mm is not optimal for reconciling diazotroph phylotypes to N2 fixation rates and that non-cyanobacterial diazotrophs may contribute importantly to bulk diazotrophic activity in the western subtropical North Atlantic. Consolider-Malaspina (CSD2008-00077), CAIBEX (CTM2007-66408- CO2-02). HOTMIX (CTM2011-30010-CO2-01) Versión del editor 1,7490 Article in Journal/Newspaper North Atlantic Instituto Español de Oceanografía: e-IEO Journal of Plankton Research 38 3 662 672 |
institution |
Open Polar |
collection |
Instituto Español de Oceanografía: e-IEO |
op_collection_id |
ftieo |
language |
English |
topic |
nifH qPCR N2 fixation upwelling North Atlantic Subtropical Gyre |
spellingShingle |
nifH qPCR N2 fixation upwelling North Atlantic Subtropical Gyre Benavides, M. (Mar) Moisander, P.H. Daley, M.C. Bode, A. (Antonio) Arístegui, J. (Javier) Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean |
topic_facet |
nifH qPCR N2 fixation upwelling North Atlantic Subtropical Gyre |
description |
Diazotrophy-related studies in the North Atlantic have largely focused on its western tropical area, leaving the subtropics and the east undersampled. We studied the longitudinal distribution of Trichodesmium, UCYN-A, UCYN-B, the putative Gammaproteobacterium g-24774A11 and Richelia (Het1) along 24.58N, using quantitative polymerase chain reaction on different size fractions (10, 10–3 and 3–0.2 mm) and additional filament counts for Trichodesmium. Trichodesmium was the most abundant phylotype, followed by UCYN-A, g-24774A11 and Het1, with maximum abundances of 8.8 ! 105, 2.0 ! 105, 3.3 ! 103 and 3.4 ! 102 nifH copies L21, respectively, whereas UCYN-B was mostly undetected. A clear shift in the diazotroph community was observed at !308W, coinciding with the transition between the North Atlantic Subtropical Gyre boundary and inner core. This transition zone divided the transect into an eastern half dominated by UCYN-A and western half dominated by Trichodesmium and g-24774A11. g-24774A11 was only detected in the 10–3 mm fraction, suggesting their association with larger microbes or aggregates. Our results indicate that typical size fractionation by 10 mm is not optimal for reconciling diazotroph phylotypes to N2 fixation rates and that non-cyanobacterial diazotrophs may contribute importantly to bulk diazotrophic activity in the western subtropical North Atlantic. Consolider-Malaspina (CSD2008-00077), CAIBEX (CTM2007-66408- CO2-02). HOTMIX (CTM2011-30010-CO2-01) Versión del editor 1,7490 |
format |
Article in Journal/Newspaper |
author |
Benavides, M. (Mar) Moisander, P.H. Daley, M.C. Bode, A. (Antonio) Arístegui, J. (Javier) |
author_facet |
Benavides, M. (Mar) Moisander, P.H. Daley, M.C. Bode, A. (Antonio) Arístegui, J. (Javier) |
author_sort |
Benavides, M. (Mar) |
title |
Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean |
title_short |
Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean |
title_full |
Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean |
title_fullStr |
Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean |
title_full_unstemmed |
Longitudinal variability of diazotroph abundances in the subtropical North Atlantic Ocean |
title_sort |
longitudinal variability of diazotroph abundances in the subtropical north atlantic ocean |
publisher |
Centro Oceanográfico de A Coruña |
publishDate |
2016 |
url |
http://hdl.handle.net/10508/10151 https://doi.org/10.1093/plankt/fbv121 |
op_coverage |
Atlantic Ocean North Atlantic |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://plankt.oxfordjournals.org/content/early/2016/01/18/plankt.fbv121.abstract?sid=16a196d0-45c3-42dc-b58f-d49abb072dd2 Consolider-Malaspina (CSD2008-00077), CAIBEX (CTM2007-66408- CO2-02), HOTMIX (CTM2011-30010-CO2-01) 0142-7873 http://hdl.handle.net/10508/10151 Journal of Plankton Research, 0. 2016: 1-11 doi:10.1093/plankt/fbv121 |
op_rights |
Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1093/plankt/fbv121 |
container_title |
Journal of Plankton Research |
container_volume |
38 |
container_issue |
3 |
container_start_page |
662 |
op_container_end_page |
672 |
_version_ |
1766118080569671680 |