Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms
Copepods have been widely used to evaluate toxicity of metals present in marine environments. However, a technical difficulty is to understand the possible routes of metal uptake and to identify in which tissues or organs metals are being accumulated. Traditional techniques are hard to be employed o...
Published in: | Chemosphere |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2011
|
Subjects: | |
Online Access: | http://hdl.handle.net/10625/49737 |
id |
ftidrcdspace:oai:idl-bnc-idrc.dspacedirect.org:10625/49737 |
---|---|
record_format |
openpolar |
spelling |
ftidrcdspace:oai:idl-bnc-idrc.dspacedirect.org:10625/49737 2023-07-16T03:57:51+02:00 Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms Pinho, G L L Bianchini, A Rouleau, C 2011 Text p. 1-6 : ill http://hdl.handle.net/10625/49737 en eng Chemosphere Pinho, G. L. L., Bianchini, A., & Rouleau, C. (2011). Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms. Chemosphere, 85 (1): 1-6. doi:10.1016/j.chemosphere.2011.06.065 0045-6535 http://hdl.handle.net/10625/49737 ACCUMULATION AUTORADIOGRAPHY CALANUS HYPERBOREUS COPPER BODY DISTRIBUTION BIOACCUMULATION Abstract 2011 ftidrcdspace 2023-06-28T22:24:51Z Copepods have been widely used to evaluate toxicity of metals present in marine environments. However, a technical difficulty is to understand the possible routes of metal uptake and to identify in which tissues or organs metals are being accumulated. Traditional techniques are hard to be employed once each organ has to be analyzed separately. Autoradiography is an alternative technique to circumvent this limitation, since metal distribution in tissues can be visualized and quantified, even in small organisms like copepods. In the present study, accumulation and distribution of 64Cu in the copepod Calanus hyperboreus was studied using autoradiography. Copepods were exposed for 2 h to copper (2.3 mg L−1; 1.08 MBq 64Cu mg−1 Cu) and then allowed to depurate for 2 h in clean seawater. Total 64Cu was determined by gamma-spectrometry after a metal exposure and a depuration period. 64Cu distribution was determined based on images generated by autoradiography. Metal accumulation was observed on all external surfaces of the copepods, being accumulated mostly on the ventral region, followed by dorsal, urossoma and internal regions. After depuration, radioactivity levels had a decrease in the sum of external body surface. Our results show that copper uptake by C. hyperboreus is fast and that a non-negligible proportion of the accumulated metal can reach internal tissues, which may lead to detrimental physiological effects. Moreover, whole-body autoradiography was demonstrated to be an efficient technique to study copper accumulation and body distribution in a very small organism such as the copepod C. hyperboreus. Article in Journal/Newspaper Calanus hyperboreus Copepods International Development Research Centre: IDRC Digital Library Chemosphere 85 1 1 6 |
institution |
Open Polar |
collection |
International Development Research Centre: IDRC Digital Library |
op_collection_id |
ftidrcdspace |
language |
English |
topic |
ACCUMULATION AUTORADIOGRAPHY CALANUS HYPERBOREUS COPPER BODY DISTRIBUTION BIOACCUMULATION |
spellingShingle |
ACCUMULATION AUTORADIOGRAPHY CALANUS HYPERBOREUS COPPER BODY DISTRIBUTION BIOACCUMULATION Pinho, G L L Bianchini, A Rouleau, C Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms |
topic_facet |
ACCUMULATION AUTORADIOGRAPHY CALANUS HYPERBOREUS COPPER BODY DISTRIBUTION BIOACCUMULATION |
description |
Copepods have been widely used to evaluate toxicity of metals present in marine environments. However, a technical difficulty is to understand the possible routes of metal uptake and to identify in which tissues or organs metals are being accumulated. Traditional techniques are hard to be employed once each organ has to be analyzed separately. Autoradiography is an alternative technique to circumvent this limitation, since metal distribution in tissues can be visualized and quantified, even in small organisms like copepods. In the present study, accumulation and distribution of 64Cu in the copepod Calanus hyperboreus was studied using autoradiography. Copepods were exposed for 2 h to copper (2.3 mg L−1; 1.08 MBq 64Cu mg−1 Cu) and then allowed to depurate for 2 h in clean seawater. Total 64Cu was determined by gamma-spectrometry after a metal exposure and a depuration period. 64Cu distribution was determined based on images generated by autoradiography. Metal accumulation was observed on all external surfaces of the copepods, being accumulated mostly on the ventral region, followed by dorsal, urossoma and internal regions. After depuration, radioactivity levels had a decrease in the sum of external body surface. Our results show that copper uptake by C. hyperboreus is fast and that a non-negligible proportion of the accumulated metal can reach internal tissues, which may lead to detrimental physiological effects. Moreover, whole-body autoradiography was demonstrated to be an efficient technique to study copper accumulation and body distribution in a very small organism such as the copepod C. hyperboreus. |
format |
Article in Journal/Newspaper |
author |
Pinho, G L L Bianchini, A Rouleau, C |
author_facet |
Pinho, G L L Bianchini, A Rouleau, C |
author_sort |
Pinho, G L L |
title |
Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms |
title_short |
Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms |
title_full |
Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms |
title_fullStr |
Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms |
title_full_unstemmed |
Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms |
title_sort |
whole-body autoradiography: an efficient technique to study copper accumulation and body distribution in small organisms |
publishDate |
2011 |
url |
http://hdl.handle.net/10625/49737 |
genre |
Calanus hyperboreus Copepods |
genre_facet |
Calanus hyperboreus Copepods |
op_relation |
Chemosphere Pinho, G. L. L., Bianchini, A., & Rouleau, C. (2011). Whole-body autoradiography: An efficient technique to study copper accumulation and body distribution in small organisms. Chemosphere, 85 (1): 1-6. doi:10.1016/j.chemosphere.2011.06.065 0045-6535 http://hdl.handle.net/10625/49737 |
container_title |
Chemosphere |
container_volume |
85 |
container_issue |
1 |
container_start_page |
1 |
op_container_end_page |
6 |
_version_ |
1771544677066997760 |