The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic)
Antarctic actinobacteria, which can be isolated from both soils and marine sediments, demonstrate a wide range of antimicrobial activities as well as significant biosynthetic potential as the producers of biologically active compounds. However, the actinobacterial diversity of the Antarctic region h...
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fthzi:oai:repository.helmholtz-hzi.de:10033/623073 2023-05-15T13:50:37+02:00 The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic) Tistechok, Stepan Skvortsova, Maryna Mytsyk, Yuliia Fedorenko, Victor Parnikoza, Ivan Luzhetskyy, Andriy Gromyko, Oleksandr HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany. 2021-09-01 http://hdl.handle.net/10033/623073 https://doi.org/10.1007/s00300-021-02924-2 en eng Springer Polar Biol 44, 1859–1868 (2021). https://doi.org/10.1007/s00300-021-02924-2. 07224060 doi:10.1007/s00300-021-02924-2 http://hdl.handle.net/10033/623073 14322056 Polar Biology 2-s2.0-85112193855 SCOPUS_ID:85112193855 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ CC-BY 44 9 1859 1868 Polar Biology Antarctic actinobacteria Biosynthetic potential Deschampsia antarctica Galindez Island Umezawaea sp Article 2021 fthzi https://doi.org/10.1007/s00300-021-02924-2 2022-03-09T17:40:41Z Antarctic actinobacteria, which can be isolated from both soils and marine sediments, demonstrate a wide range of antimicrobial activities as well as significant biosynthetic potential as the producers of biologically active compounds. However, the actinobacterial diversity of the Antarctic region has not yet been sufficiently studied. The present study sought to examine the diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (É. Desv.), which was collected from Galindez Island, Maritime Antarctic. Among the actinobacteria isolated using a 16S rRNA gene sequence-based phylogenetic analysis process, five genera, namely Streptomyces, Micromonospora, Umezawaea, Kribbella and Micrococcus, were identified. To the best of our knowledge, this is the first report to describe the isolation and initial characterisation of members of the genus Umezawaea from the Antarctic. The isolated actinobacteria were assayed to determine their activity against Gram-positive bacteria, Gram-negative bacteria and yeast. Among the isolated strains, only 30.2% were able to inhibit the growth of at least one of the tested pathogens. The polymerase chain reaction-based screening of the biosynthetic genes revealed the presence of type I polyketide synthases (65.1%), type II polyketide synthases (25.6%) and non-ribosomal peptide synthetases (9.3%) in the actinobacteria strains. The examination of the sensitivity/resistance to antibiotics profile of the actinobacteria strains revealed their high sensitivity in relation to the tested antibiotics. Taken together, the results showed that Antarctic actinobacteria demonstrate potential as the producers of natural bioactive compounds, which means that they represent a valuable prospect for further studies. Ministry of Education and Science of Ukraine Article in Journal/Newspaper Antarc* Antarctic Antarctica Galindez Island Polar Biology Helmholtz Centre of Infection Research (HZI), Braunschweig: Repository Antarctic The Antarctic Galindez ENVELOPE(-64.248,-64.248,-65.250,-65.250) Galindez Island ENVELOPE(-64.267,-64.267,-65.250,-65.250) Polar Biology 44 9 1859 1868 |
institution |
Open Polar |
collection |
Helmholtz Centre of Infection Research (HZI), Braunschweig: Repository |
op_collection_id |
fthzi |
language |
English |
topic |
Antarctic actinobacteria Biosynthetic potential Deschampsia antarctica Galindez Island Umezawaea sp |
spellingShingle |
Antarctic actinobacteria Biosynthetic potential Deschampsia antarctica Galindez Island Umezawaea sp Tistechok, Stepan Skvortsova, Maryna Mytsyk, Yuliia Fedorenko, Victor Parnikoza, Ivan Luzhetskyy, Andriy Gromyko, Oleksandr The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic) |
topic_facet |
Antarctic actinobacteria Biosynthetic potential Deschampsia antarctica Galindez Island Umezawaea sp |
description |
Antarctic actinobacteria, which can be isolated from both soils and marine sediments, demonstrate a wide range of antimicrobial activities as well as significant biosynthetic potential as the producers of biologically active compounds. However, the actinobacterial diversity of the Antarctic region has not yet been sufficiently studied. The present study sought to examine the diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (É. Desv.), which was collected from Galindez Island, Maritime Antarctic. Among the actinobacteria isolated using a 16S rRNA gene sequence-based phylogenetic analysis process, five genera, namely Streptomyces, Micromonospora, Umezawaea, Kribbella and Micrococcus, were identified. To the best of our knowledge, this is the first report to describe the isolation and initial characterisation of members of the genus Umezawaea from the Antarctic. The isolated actinobacteria were assayed to determine their activity against Gram-positive bacteria, Gram-negative bacteria and yeast. Among the isolated strains, only 30.2% were able to inhibit the growth of at least one of the tested pathogens. The polymerase chain reaction-based screening of the biosynthetic genes revealed the presence of type I polyketide synthases (65.1%), type II polyketide synthases (25.6%) and non-ribosomal peptide synthetases (9.3%) in the actinobacteria strains. The examination of the sensitivity/resistance to antibiotics profile of the actinobacteria strains revealed their high sensitivity in relation to the tested antibiotics. Taken together, the results showed that Antarctic actinobacteria demonstrate potential as the producers of natural bioactive compounds, which means that they represent a valuable prospect for further studies. Ministry of Education and Science of Ukraine |
author2 |
HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany. |
format |
Article in Journal/Newspaper |
author |
Tistechok, Stepan Skvortsova, Maryna Mytsyk, Yuliia Fedorenko, Victor Parnikoza, Ivan Luzhetskyy, Andriy Gromyko, Oleksandr |
author_facet |
Tistechok, Stepan Skvortsova, Maryna Mytsyk, Yuliia Fedorenko, Victor Parnikoza, Ivan Luzhetskyy, Andriy Gromyko, Oleksandr |
author_sort |
Tistechok, Stepan |
title |
The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic) |
title_short |
The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic) |
title_full |
The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic) |
title_fullStr |
The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic) |
title_full_unstemmed |
The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic) |
title_sort |
diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of deschampsia antarctica (galindez island, maritime antarctic) |
publisher |
Springer |
publishDate |
2021 |
url |
http://hdl.handle.net/10033/623073 https://doi.org/10.1007/s00300-021-02924-2 |
long_lat |
ENVELOPE(-64.248,-64.248,-65.250,-65.250) ENVELOPE(-64.267,-64.267,-65.250,-65.250) |
geographic |
Antarctic The Antarctic Galindez Galindez Island |
geographic_facet |
Antarctic The Antarctic Galindez Galindez Island |
genre |
Antarc* Antarctic Antarctica Galindez Island Polar Biology |
genre_facet |
Antarc* Antarctic Antarctica Galindez Island Polar Biology |
op_source |
44 9 1859 1868 Polar Biology |
op_relation |
Polar Biol 44, 1859–1868 (2021). https://doi.org/10.1007/s00300-021-02924-2. 07224060 doi:10.1007/s00300-021-02924-2 http://hdl.handle.net/10033/623073 14322056 Polar Biology 2-s2.0-85112193855 SCOPUS_ID:85112193855 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00300-021-02924-2 |
container_title |
Polar Biology |
container_volume |
44 |
container_issue |
9 |
container_start_page |
1859 |
op_container_end_page |
1868 |
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1766253768787099648 |