Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica.
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis-the paradigm of mesophilic hydrocarbonoclastic bacteria-O. ant...
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Online Access: | http://hdl.handle.net/10033/299523 https://doi.org/10.1038/ncomms3156 |
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fthzi:oai:repository.helmholtz-hzi.de:10033/299523 2023-05-15T13:50:37+02:00 Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. Kube, Michael Chernikova, Tatyana N Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, María-Eugenia Heipieper, Hermann J Klages, Sven Kotsyurbenko, Oleg R Langer, Ines Nechitaylo, Taras Y Lünsdorf, Heinrich Fernández, Marisol Juárez, Silvia Ciordia, Sergio Singer, Alexander Kagan, Olga Egorova, Olga Alain Petit, Pierre Stogios, Peter Kim, Youngchang Tchigvintsev, Anatoli Flick, Robert Denaro, Renata Genovese, Maria Albar, Juan P Reva, Oleg N Martínez-Gomariz, Montserrat Tran, Hai Ferrer, Manuel Savchenko, Alexei Yakunin, Alexander F Yakimov, Michail M Golyshina, Olga V Reinhardt, Richard Golyshin, Peter N 1 Max-Planck Institute for Molecular Genetics, Berlin-Dahlem D-14195, Germany 2 Section Phytomedicine, Department of Crop and Animal Sciences, Humboldt-Universität zu Berlin, Berlin-Dahlem D-14195, Germany. 2013-07-23 http://hdl.handle.net/10033/299523 https://doi.org/10.1038/ncomms3156 en eng Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. 2013, 4:2156 Nat Commun 2041-1723 23877221 doi:10.1038/ncomms3156 http://hdl.handle.net/10033/299523 Nature communications Archived with thanks to Nature communications Article 2013 fthzi https://doi.org/10.1038/ncomms3156 2022-03-09T17:39:56Z Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis-the paradigm of mesophilic hydrocarbonoclastic bacteria-O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. Article in Journal/Newspaper Antarc* Antarctica Helmholtz Centre of Infection Research (HZI), Braunschweig: Repository Nature Communications 4 1 |
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Helmholtz Centre of Infection Research (HZI), Braunschweig: Repository |
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fthzi |
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English |
description |
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis-the paradigm of mesophilic hydrocarbonoclastic bacteria-O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. |
author2 |
1 Max-Planck Institute for Molecular Genetics, Berlin-Dahlem D-14195, Germany 2 Section Phytomedicine, Department of Crop and Animal Sciences, Humboldt-Universität zu Berlin, Berlin-Dahlem D-14195, Germany. |
format |
Article in Journal/Newspaper |
author |
Kube, Michael Chernikova, Tatyana N Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, María-Eugenia Heipieper, Hermann J Klages, Sven Kotsyurbenko, Oleg R Langer, Ines Nechitaylo, Taras Y Lünsdorf, Heinrich Fernández, Marisol Juárez, Silvia Ciordia, Sergio Singer, Alexander Kagan, Olga Egorova, Olga Alain Petit, Pierre Stogios, Peter Kim, Youngchang Tchigvintsev, Anatoli Flick, Robert Denaro, Renata Genovese, Maria Albar, Juan P Reva, Oleg N Martínez-Gomariz, Montserrat Tran, Hai Ferrer, Manuel Savchenko, Alexei Yakunin, Alexander F Yakimov, Michail M Golyshina, Olga V Reinhardt, Richard Golyshin, Peter N |
spellingShingle |
Kube, Michael Chernikova, Tatyana N Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, María-Eugenia Heipieper, Hermann J Klages, Sven Kotsyurbenko, Oleg R Langer, Ines Nechitaylo, Taras Y Lünsdorf, Heinrich Fernández, Marisol Juárez, Silvia Ciordia, Sergio Singer, Alexander Kagan, Olga Egorova, Olga Alain Petit, Pierre Stogios, Peter Kim, Youngchang Tchigvintsev, Anatoli Flick, Robert Denaro, Renata Genovese, Maria Albar, Juan P Reva, Oleg N Martínez-Gomariz, Montserrat Tran, Hai Ferrer, Manuel Savchenko, Alexei Yakunin, Alexander F Yakimov, Michail M Golyshina, Olga V Reinhardt, Richard Golyshin, Peter N Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. |
author_facet |
Kube, Michael Chernikova, Tatyana N Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, María-Eugenia Heipieper, Hermann J Klages, Sven Kotsyurbenko, Oleg R Langer, Ines Nechitaylo, Taras Y Lünsdorf, Heinrich Fernández, Marisol Juárez, Silvia Ciordia, Sergio Singer, Alexander Kagan, Olga Egorova, Olga Alain Petit, Pierre Stogios, Peter Kim, Youngchang Tchigvintsev, Anatoli Flick, Robert Denaro, Renata Genovese, Maria Albar, Juan P Reva, Oleg N Martínez-Gomariz, Montserrat Tran, Hai Ferrer, Manuel Savchenko, Alexei Yakunin, Alexander F Yakimov, Michail M Golyshina, Olga V Reinhardt, Richard Golyshin, Peter N |
author_sort |
Kube, Michael |
title |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. |
title_short |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. |
title_full |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. |
title_fullStr |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. |
title_full_unstemmed |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. |
title_sort |
genome sequence and functional genomic analysis of the oil-degrading bacterium oleispira antarctica. |
publishDate |
2013 |
url |
http://hdl.handle.net/10033/299523 https://doi.org/10.1038/ncomms3156 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. 2013, 4:2156 Nat Commun 2041-1723 23877221 doi:10.1038/ncomms3156 http://hdl.handle.net/10033/299523 Nature communications |
op_rights |
Archived with thanks to Nature communications |
op_doi |
https://doi.org/10.1038/ncomms3156 |
container_title |
Nature Communications |
container_volume |
4 |
container_issue |
1 |
_version_ |
1766253768387592192 |