Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS
Harbour seals (Phoca vitulina) are bio indicators for the assessment of their habitat and environmental changes. Besides population parameters and trends (survival, age structure, sex ratio), the individual health status is an important parameter for this assessment. The health status of seals is a...
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Royal Society of Chemistry
2011
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fthzgzmk:oai:publications.hereon.de:28699 2023-06-11T04:16:00+02:00 Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS Grebe, M. Pröfrock, D. Kakuschke, A. Broekaert, J.A.C. Prange, A. 2011 https://publications.hereon.de/id/28699 https://publications.hzg.de/id/28699 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2011/grebe_28699.pdf en eng Royal Society of Chemistry http://dx.doi.org/10.1039/c0mt00076k urn:issn:1756-5901 https://publications.hereon.de/id/28699 https://publications.hzg.de/id/28699 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2011/grebe_28699.pdf info:eu-repo/semantics/openAccess open_access oa_gruen issn:1756-5901 Grebe, M.; Pröfrock, D.; Kakuschke, A.; Broekaert, J.A.C.; Prange, A.: Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS. In: Metallomics. Vol. 3 (2011) 2, 176 - 185. (DOI:10.1039/c0mt00076k) info:eu-repo/classification/ddc/551 info:eu-repo/semantics/article Zeitschrift Artikel 2011 fthzgzmk https://doi.org/10.1039/c0mt00076k 2023-05-28T23:23:15Z Harbour seals (Phoca vitulina) are bio indicators for the assessment of their habitat and environmental changes. Besides population parameters and trends (survival, age structure, sex ratio), the individual health status is an important parameter for this assessment. The health status of seals is a complex and vague item, determined by a wide range of diagnostic parameters. Quantities of important blood proteins such as transferrin (Tf), as well as altered distribution patterns of its glycoforms, are frequently used as biomarkers in clinical diagnosis. Here Tf quantities and varying pattern of its glycoforms are used as indicator for e.g. a general impairment of the liver as well as certain liver diseases, which represents one of the most frequently observed pathological indication in harbour seals of the North Sea. Currently, most assay based quantification methods for Tf are limited since they often provide only information of the total Tf concentration rather than its different isoforms or deliver only relative concentrations of the Tf isoforms. Due to the lack of suitable antibodies for seal Tf also the application of more specific antibody based approaches is not possible. Within this background a new approach for the absolute quantification of the iron-transport protein Tf in the blood of harbour seals using its characteristic iron content and HPLC-ICP-MS detection is described. Method validation was performed using a certified human serum reference material (ERM-DA470K/IFCC). A Tf concentration of 2.33 ± 0.03 g L-1 (sum of all quantified isoforms) has been calculated, which is in good agreement with the certified total Tf concentration of 2.35 ± 0.08 g L-1, confirming the accuracy of the proposed analytical method. Finally, different seal samples were analysed to demonstrate the suitability of the method for the quantification of Tf in real samples as well as to observe modified glycoform patterns. A baseline reference range of 1.50 – 2.44 g L-1 Tf in serum and 0.00 – 0.18 g L-1 CDT in serum of harbour ... Article in Journal/Newspaper Phoca vitulina Hereon Publications (Helmholtz-Zentrum) Metallomics 3 2 176 |
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Hereon Publications (Helmholtz-Zentrum) |
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English |
topic |
info:eu-repo/classification/ddc/551 |
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info:eu-repo/classification/ddc/551 Grebe, M. Pröfrock, D. Kakuschke, A. Broekaert, J.A.C. Prange, A. Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS |
topic_facet |
info:eu-repo/classification/ddc/551 |
description |
Harbour seals (Phoca vitulina) are bio indicators for the assessment of their habitat and environmental changes. Besides population parameters and trends (survival, age structure, sex ratio), the individual health status is an important parameter for this assessment. The health status of seals is a complex and vague item, determined by a wide range of diagnostic parameters. Quantities of important blood proteins such as transferrin (Tf), as well as altered distribution patterns of its glycoforms, are frequently used as biomarkers in clinical diagnosis. Here Tf quantities and varying pattern of its glycoforms are used as indicator for e.g. a general impairment of the liver as well as certain liver diseases, which represents one of the most frequently observed pathological indication in harbour seals of the North Sea. Currently, most assay based quantification methods for Tf are limited since they often provide only information of the total Tf concentration rather than its different isoforms or deliver only relative concentrations of the Tf isoforms. Due to the lack of suitable antibodies for seal Tf also the application of more specific antibody based approaches is not possible. Within this background a new approach for the absolute quantification of the iron-transport protein Tf in the blood of harbour seals using its characteristic iron content and HPLC-ICP-MS detection is described. Method validation was performed using a certified human serum reference material (ERM-DA470K/IFCC). A Tf concentration of 2.33 ± 0.03 g L-1 (sum of all quantified isoforms) has been calculated, which is in good agreement with the certified total Tf concentration of 2.35 ± 0.08 g L-1, confirming the accuracy of the proposed analytical method. Finally, different seal samples were analysed to demonstrate the suitability of the method for the quantification of Tf in real samples as well as to observe modified glycoform patterns. A baseline reference range of 1.50 – 2.44 g L-1 Tf in serum and 0.00 – 0.18 g L-1 CDT in serum of harbour ... |
format |
Article in Journal/Newspaper |
author |
Grebe, M. Pröfrock, D. Kakuschke, A. Broekaert, J.A.C. Prange, A. |
author_facet |
Grebe, M. Pröfrock, D. Kakuschke, A. Broekaert, J.A.C. Prange, A. |
author_sort |
Grebe, M. |
title |
Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS |
title_short |
Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS |
title_full |
Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS |
title_fullStr |
Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS |
title_full_unstemmed |
Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS |
title_sort |
absolute quantification of transferrin in blood samples of harbour seals using hplc-icp-ms |
publisher |
Royal Society of Chemistry |
publishDate |
2011 |
url |
https://publications.hereon.de/id/28699 https://publications.hzg.de/id/28699 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2011/grebe_28699.pdf |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
issn:1756-5901 Grebe, M.; Pröfrock, D.; Kakuschke, A.; Broekaert, J.A.C.; Prange, A.: Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS. In: Metallomics. Vol. 3 (2011) 2, 176 - 185. (DOI:10.1039/c0mt00076k) |
op_relation |
http://dx.doi.org/10.1039/c0mt00076k urn:issn:1756-5901 https://publications.hereon.de/id/28699 https://publications.hzg.de/id/28699 http://www.hzg.de/imperia/md/content/gkss/zentrale_einrichtungen/bibliothek/journals/2011/grebe_28699.pdf |
op_rights |
info:eu-repo/semantics/openAccess open_access oa_gruen |
op_doi |
https://doi.org/10.1039/c0mt00076k |
container_title |
Metallomics |
container_volume |
3 |
container_issue |
2 |
container_start_page |
176 |
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1768373333925036032 |