Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula)
Since Emaraviruses have been discovered in 2007 several new species were detected in a range of host plants. Five genome segments of a novel Emaravirus from mosaic‐diseased Eurasian aspen (Populus tremula) have been completely determined. The monocistronic, segmented ssRNA genome of the virus shows...
Published in: | Annals of Applied Biology |
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Language: | English |
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Humboldt-Universität zu Berlin
2020
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Online Access: | http://edoc.hu-berlin.de/18452/22045 https://nbn-resolving.org/urn:nbn:de:kobv:11-110-18452/22045-3 https://doi.org/10.18452/21304 https://doi.org/10.1111/aab.12576 |
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fthuberlin:oai:edoc.hu-berlin.de:18452/22045 2023-12-03T10:22:31+01:00 Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula) von Bargen, Susanne Al Kubrusli, Rim Gaskin, Thomas Fürl, Stephanie Hüttner, Florian Blystad, Dag-Ragnar Karlin, David G. Jalkanen, Risto Büttner, Carmen 2020-02-11 application/pdf http://edoc.hu-berlin.de/18452/22045 https://nbn-resolving.org/urn:nbn:de:kobv:11-110-18452/22045-3 https://doi.org/10.18452/21304 https://doi.org/10.1111/aab.12576 eng eng Humboldt-Universität zu Berlin http://edoc.hu-berlin.de/18452/22045 urn:nbn:de:kobv:11-110-18452/22045-3 http://dx.doi.org/10.18452/21304 1744-7348 doi:10.1111/aab.12576 (CC BY 4.0) Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Aspen mosaic-associated virus genome organisation geographic distribution graft transmission 570 Biologie ddc:570 article doc-type:article publishedVersion 2020 fthuberlin https://doi.org/10.18452/2130410.1111/aab.12576 2023-11-05T23:35:59Z Since Emaraviruses have been discovered in 2007 several new species were detected in a range of host plants. Five genome segments of a novel Emaravirus from mosaic‐diseased Eurasian aspen (Populus tremula) have been completely determined. The monocistronic, segmented ssRNA genome of the virus shows a genome organisation typical for Emaraviruses encoding the viral RNA‐dependent RNA polymerase (RdRP, 268.2 kDa) on RNA1 (7.1 kb), a glycoprotein precursor (GPP, 73.5 kDa) on RNA2 (2.3 kb), the viral nucleocapsid protein (N, 35.6 kDa) on RNA3 (1.6 kb), and a putative movement protein (MP, 41.0 kDa) on RNA4 (1.6 kb). The fifth identified genome segment (RNA5, 1.3 kb) encodes a protein of unknown function (P28, 28.1 kDa). We discovered that it is distantly related to proteins encoded by Emaraviruses, such as P4 of European mountain ash ringspot‐associated virus. All proteins from this group contain a central hydrophobic region with a conserved secondary structure and a hydrophobic amino acid stretch, bordered by two highly conserved positions, thus clearly representing a new group of homologues of Emaraviruses. The virus identified in Eurasian aspen is closely associated with observed leaf symptoms, such as mottle, yellow blotching, variegation and chloroses along veins. All five viral RNAs were regularly detectable by RT‐PCR in mosaic‐diseased P. tremula in Norway, Finland and Sweden (Fennoscandia). Observed symptoms and testing of mosaic‐diseased Eurasian aspen by virus‐specific RT‐PCR targeting RNA3 and RNA4 confirmed a wide geographic distribution of the virus in Fennoscandia. We could demonstrate that the mosaic‐disease is graft‐transmissible and confirmed that the virus is the causal agent by detection in symptomatic, graft‐inoculated seedlings used as rootstocks as well as in the virus‐infected scions used for graft‐inoculation. Owing to these characteristics, the virus represents a novel species within the genus Emaravirus and was tentatively denominated aspen mosaic‐associated virus. Peer Reviewed Article in Journal/Newspaper Fennoscandia Open-Access-Publikationsserver der Humboldt-Universität: edoc-Server Norway Annals of Applied Biology 176 2 210 222 |
institution |
Open Polar |
collection |
Open-Access-Publikationsserver der Humboldt-Universität: edoc-Server |
op_collection_id |
fthuberlin |
language |
English |
topic |
Aspen mosaic-associated virus genome organisation geographic distribution graft transmission 570 Biologie ddc:570 |
spellingShingle |
Aspen mosaic-associated virus genome organisation geographic distribution graft transmission 570 Biologie ddc:570 von Bargen, Susanne Al Kubrusli, Rim Gaskin, Thomas Fürl, Stephanie Hüttner, Florian Blystad, Dag-Ragnar Karlin, David G. Jalkanen, Risto Büttner, Carmen Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula) |
topic_facet |
Aspen mosaic-associated virus genome organisation geographic distribution graft transmission 570 Biologie ddc:570 |
description |
Since Emaraviruses have been discovered in 2007 several new species were detected in a range of host plants. Five genome segments of a novel Emaravirus from mosaic‐diseased Eurasian aspen (Populus tremula) have been completely determined. The monocistronic, segmented ssRNA genome of the virus shows a genome organisation typical for Emaraviruses encoding the viral RNA‐dependent RNA polymerase (RdRP, 268.2 kDa) on RNA1 (7.1 kb), a glycoprotein precursor (GPP, 73.5 kDa) on RNA2 (2.3 kb), the viral nucleocapsid protein (N, 35.6 kDa) on RNA3 (1.6 kb), and a putative movement protein (MP, 41.0 kDa) on RNA4 (1.6 kb). The fifth identified genome segment (RNA5, 1.3 kb) encodes a protein of unknown function (P28, 28.1 kDa). We discovered that it is distantly related to proteins encoded by Emaraviruses, such as P4 of European mountain ash ringspot‐associated virus. All proteins from this group contain a central hydrophobic region with a conserved secondary structure and a hydrophobic amino acid stretch, bordered by two highly conserved positions, thus clearly representing a new group of homologues of Emaraviruses. The virus identified in Eurasian aspen is closely associated with observed leaf symptoms, such as mottle, yellow blotching, variegation and chloroses along veins. All five viral RNAs were regularly detectable by RT‐PCR in mosaic‐diseased P. tremula in Norway, Finland and Sweden (Fennoscandia). Observed symptoms and testing of mosaic‐diseased Eurasian aspen by virus‐specific RT‐PCR targeting RNA3 and RNA4 confirmed a wide geographic distribution of the virus in Fennoscandia. We could demonstrate that the mosaic‐disease is graft‐transmissible and confirmed that the virus is the causal agent by detection in symptomatic, graft‐inoculated seedlings used as rootstocks as well as in the virus‐infected scions used for graft‐inoculation. Owing to these characteristics, the virus represents a novel species within the genus Emaravirus and was tentatively denominated aspen mosaic‐associated virus. Peer Reviewed |
format |
Article in Journal/Newspaper |
author |
von Bargen, Susanne Al Kubrusli, Rim Gaskin, Thomas Fürl, Stephanie Hüttner, Florian Blystad, Dag-Ragnar Karlin, David G. Jalkanen, Risto Büttner, Carmen |
author_facet |
von Bargen, Susanne Al Kubrusli, Rim Gaskin, Thomas Fürl, Stephanie Hüttner, Florian Blystad, Dag-Ragnar Karlin, David G. Jalkanen, Risto Büttner, Carmen |
author_sort |
von Bargen, Susanne |
title |
Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula) |
title_short |
Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula) |
title_full |
Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula) |
title_fullStr |
Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula) |
title_full_unstemmed |
Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula) |
title_sort |
characterisation of a novel emaravirus identified in mosaic‐diseased eurasian aspen (populus tremula) |
publisher |
Humboldt-Universität zu Berlin |
publishDate |
2020 |
url |
http://edoc.hu-berlin.de/18452/22045 https://nbn-resolving.org/urn:nbn:de:kobv:11-110-18452/22045-3 https://doi.org/10.18452/21304 https://doi.org/10.1111/aab.12576 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Fennoscandia |
genre_facet |
Fennoscandia |
op_relation |
http://edoc.hu-berlin.de/18452/22045 urn:nbn:de:kobv:11-110-18452/22045-3 http://dx.doi.org/10.18452/21304 1744-7348 doi:10.1111/aab.12576 |
op_rights |
(CC BY 4.0) Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.18452/2130410.1111/aab.12576 |
container_title |
Annals of Applied Biology |
container_volume |
176 |
container_issue |
2 |
container_start_page |
210 |
op_container_end_page |
222 |
_version_ |
1784270456009785344 |