Characterization of the impact of dietary immunostimulant CpG on the expression of mRNA biomarkers involved in the immune responses in Atlantic salmon (Salmo salar)
Infectious diseases have significantly impacted Atlantic salmon aquaculture worldwide. Modulating fish immunity with immunostimulant-containing functional feeds could be an effective strategy in mitigating disease problems. Previously, we characterized the impact of polyriboinosinic polyribocytidyli...
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fthsosloakersoda:oai:oda.oslomet.no:11250/3149178 2024-09-15T17:55:54+00:00 Characterization of the impact of dietary immunostimulant CpG on the expression of mRNA biomarkers involved in the immune responses in Atlantic salmon (Salmo salar) Xue, Xi Eslamloo, Khalil Andreassen, Rune Rise, Matthew L. 2024-08-28T12:32:44Z application/pdf https://hdl.handle.net/11250/3149178 https://doi.org/10.1016/j.fsi.2024.109840 eng eng Norges forskningsråd: 280839 Fish and Shellfish Immunology. 2024, . urn:issn:1050-4648 https://hdl.handle.net/11250/3149178 https://doi.org/10.1016/j.fsi.2024.109840 cristin:2290065 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Fish and Shellfish Immunology 15 Peer reviewed Journal article 2024 fthsosloakersoda https://doi.org/10.1016/j.fsi.2024.109840 2024-09-03T23:35:26Z Infectious diseases have significantly impacted Atlantic salmon aquaculture worldwide. Modulating fish immunity with immunostimulant-containing functional feeds could be an effective strategy in mitigating disease problems. Previously, we characterized the impact of polyriboinosinic polyribocytidylic acid (pIC) and formalinkilled typical Aeromonas salmonicida bacterin on miRNA expression in Atlantic salmon fed a commercial diet with and without immunostimulant CpG. A set of miRNA biomarkers of Atlantic salmon head kidney responding to pIC and/or bacterin immune stimulations was identified (Xue et al., 2019) [1]. Herein, we report a complementary qPCR study that investigated the impact of the pIC, bacterin and dietary CpG on the expression of immune-relevant mRNAs (n = 31) using the same samples as in the previous study (Xue et al., 2019) [1]. Twenty-six of these genes were predicted target transcripts of the pIC- and/or bacterin-responsive miRNAs identified in the earlier study. The current data showed that pIC and/or bacterin stimulations significantly modulated the majority of the qPCR-analyzed genes involved in various immune pathways. Some genes responded to both stimulations (e.g. tnfa, il10rb, ifng, irf9, cxcr3, campb) while others appeared to be stimulation specific [e.g. irf3, irf7a, il1r1, mxa, mapk3 (pIC only); clra (bacterin only)]. A. salmonicida bacterin stimulation produced a strong inflammatory response (e.g. higher expression of il1b, il8a and tnfa), while salmon stimulated with pIC showed robust interferon responses (both type I and II). Furthermore, the current data indicated significant down-regulation of immune-relevant transcripts (e.g. tlr9, irf5, il1r1, hsp90ab1, itgb2) by dietary immunostimulant CpG, especially among pre-injection and PBS-injected fish. Together with our prior miRNA study, the present research provided complementary information on Atlantic salmon anti-viral and anti-bacterial immune responses and on how dietary CpG may modulate these responses. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar OsloMet (Oslo Metropolitan University): ODA (Open Digital Archive) |
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OsloMet (Oslo Metropolitan University): ODA (Open Digital Archive) |
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fthsosloakersoda |
language |
English |
description |
Infectious diseases have significantly impacted Atlantic salmon aquaculture worldwide. Modulating fish immunity with immunostimulant-containing functional feeds could be an effective strategy in mitigating disease problems. Previously, we characterized the impact of polyriboinosinic polyribocytidylic acid (pIC) and formalinkilled typical Aeromonas salmonicida bacterin on miRNA expression in Atlantic salmon fed a commercial diet with and without immunostimulant CpG. A set of miRNA biomarkers of Atlantic salmon head kidney responding to pIC and/or bacterin immune stimulations was identified (Xue et al., 2019) [1]. Herein, we report a complementary qPCR study that investigated the impact of the pIC, bacterin and dietary CpG on the expression of immune-relevant mRNAs (n = 31) using the same samples as in the previous study (Xue et al., 2019) [1]. Twenty-six of these genes were predicted target transcripts of the pIC- and/or bacterin-responsive miRNAs identified in the earlier study. The current data showed that pIC and/or bacterin stimulations significantly modulated the majority of the qPCR-analyzed genes involved in various immune pathways. Some genes responded to both stimulations (e.g. tnfa, il10rb, ifng, irf9, cxcr3, campb) while others appeared to be stimulation specific [e.g. irf3, irf7a, il1r1, mxa, mapk3 (pIC only); clra (bacterin only)]. A. salmonicida bacterin stimulation produced a strong inflammatory response (e.g. higher expression of il1b, il8a and tnfa), while salmon stimulated with pIC showed robust interferon responses (both type I and II). Furthermore, the current data indicated significant down-regulation of immune-relevant transcripts (e.g. tlr9, irf5, il1r1, hsp90ab1, itgb2) by dietary immunostimulant CpG, especially among pre-injection and PBS-injected fish. Together with our prior miRNA study, the present research provided complementary information on Atlantic salmon anti-viral and anti-bacterial immune responses and on how dietary CpG may modulate these responses. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Xue, Xi Eslamloo, Khalil Andreassen, Rune Rise, Matthew L. |
spellingShingle |
Xue, Xi Eslamloo, Khalil Andreassen, Rune Rise, Matthew L. Characterization of the impact of dietary immunostimulant CpG on the expression of mRNA biomarkers involved in the immune responses in Atlantic salmon (Salmo salar) |
author_facet |
Xue, Xi Eslamloo, Khalil Andreassen, Rune Rise, Matthew L. |
author_sort |
Xue, Xi |
title |
Characterization of the impact of dietary immunostimulant CpG on the expression of mRNA biomarkers involved in the immune responses in Atlantic salmon (Salmo salar) |
title_short |
Characterization of the impact of dietary immunostimulant CpG on the expression of mRNA biomarkers involved in the immune responses in Atlantic salmon (Salmo salar) |
title_full |
Characterization of the impact of dietary immunostimulant CpG on the expression of mRNA biomarkers involved in the immune responses in Atlantic salmon (Salmo salar) |
title_fullStr |
Characterization of the impact of dietary immunostimulant CpG on the expression of mRNA biomarkers involved in the immune responses in Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Characterization of the impact of dietary immunostimulant CpG on the expression of mRNA biomarkers involved in the immune responses in Atlantic salmon (Salmo salar) |
title_sort |
characterization of the impact of dietary immunostimulant cpg on the expression of mrna biomarkers involved in the immune responses in atlantic salmon (salmo salar) |
publishDate |
2024 |
url |
https://hdl.handle.net/11250/3149178 https://doi.org/10.1016/j.fsi.2024.109840 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Fish and Shellfish Immunology 15 |
op_relation |
Norges forskningsråd: 280839 Fish and Shellfish Immunology. 2024, . urn:issn:1050-4648 https://hdl.handle.net/11250/3149178 https://doi.org/10.1016/j.fsi.2024.109840 cristin:2290065 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no |
op_doi |
https://doi.org/10.1016/j.fsi.2024.109840 |
_version_ |
1810432129553661952 |