Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study
Scope. To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results. Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocalori...
Published in: | Molecular Nutrition & Food Research |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Wiley
2020
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Online Access: | https://hdl.handle.net/10642/8056 https://doi.org/10.1002/mnfr.201801405 |
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fthsosloakersoda:oai:oda.oslomet.no:10642/8056 |
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OsloMet (Oslo Metropolitan University): ODA (Open Digital Archive) |
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language |
English |
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Gene expressions Healthy nordic diets Metabolic syndromes Peripheralblood mononuclear cells Transcriptomes |
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Gene expressions Healthy nordic diets Metabolic syndromes Peripheralblood mononuclear cells Transcriptomes Myhrstad, Mari De Mello, Vanessa D. Dahlman, Ingrid Kolehmainen, Marjukka Paananen, Jussi Rundblad, Amanda Carlberg, Carsten Olstad, Ole Kristoffer Pihlajamaki, Jussi Holven, Kirsten Bjørklund Hermansen, Kjeld Dragsted, Lars O. Gunnarsdottir, Ingibjörg Cloetens, Lieselotte Storm, Matilda Ulmius Åkesson, Björn Rosqvist, Fredrik Hukkanen, Janne Herzig, Karl-Heinz Risérus, Ulf Thorsdottir, Inga Poutanen, Kaisa S. Savolainen, Markku J. Schwab, Ursula Arner, Peter Uusitupa, Matti Ulven, Stine Marie Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study |
topic_facet |
Gene expressions Healthy nordic diets Metabolic syndromes Peripheralblood mononuclear cells Transcriptomes |
description |
Scope. To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results. Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p‐value < 0.05). Twenty‐five of these are significantly regulated (FDR q‐value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF‐κB, are downregulated in the healthy Nordic diet group. Conclusion. These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation. The project was funded by NordForsk Nordic Centre of Excellencein Food, Nutrition and Health project 070014 (SYSDIET [Systems Biologyin Controlled Dietary Interventions and Cohort Studies]) and further, OsloMetropolitan University-OsloMet (Norway), University of Oslo (Norway),Throne Holst Foundation (Norway), Academy of Finland, SwedishResearch Council, Svenska Diabetesf ̈orbundet, SRP Diabetes, FinnishDiabetes Research Foundation, Finnish Foundation for CardiovascularResearch, the Sigrid Juselius Foundation, EVO funding from KuopioUniversity Hospital (Finland), the Druvan Foundation, Sk ̊ane UniversityHospital, the Heart-Lung Foundation, Diabetesfonden and FoundationCerealia (Sweden), the Danish Obesity Research Centre (DanORC,www.danorc.dk), the Danish Council for Strategic Research (DairyHealth,BioFunCarb) (Denmark), the Agricultural Productivity Fund, and theResearch Fund of the University of Iceland (Iceland). acceptedVersion |
format |
Article in Journal/Newspaper |
author |
Myhrstad, Mari De Mello, Vanessa D. Dahlman, Ingrid Kolehmainen, Marjukka Paananen, Jussi Rundblad, Amanda Carlberg, Carsten Olstad, Ole Kristoffer Pihlajamaki, Jussi Holven, Kirsten Bjørklund Hermansen, Kjeld Dragsted, Lars O. Gunnarsdottir, Ingibjörg Cloetens, Lieselotte Storm, Matilda Ulmius Åkesson, Björn Rosqvist, Fredrik Hukkanen, Janne Herzig, Karl-Heinz Risérus, Ulf Thorsdottir, Inga Poutanen, Kaisa S. Savolainen, Markku J. Schwab, Ursula Arner, Peter Uusitupa, Matti Ulven, Stine Marie |
author_facet |
Myhrstad, Mari De Mello, Vanessa D. Dahlman, Ingrid Kolehmainen, Marjukka Paananen, Jussi Rundblad, Amanda Carlberg, Carsten Olstad, Ole Kristoffer Pihlajamaki, Jussi Holven, Kirsten Bjørklund Hermansen, Kjeld Dragsted, Lars O. Gunnarsdottir, Ingibjörg Cloetens, Lieselotte Storm, Matilda Ulmius Åkesson, Björn Rosqvist, Fredrik Hukkanen, Janne Herzig, Karl-Heinz Risérus, Ulf Thorsdottir, Inga Poutanen, Kaisa S. Savolainen, Markku J. Schwab, Ursula Arner, Peter Uusitupa, Matti Ulven, Stine Marie |
author_sort |
Myhrstad, Mari |
title |
Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study |
title_short |
Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study |
title_full |
Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study |
title_fullStr |
Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study |
title_full_unstemmed |
Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study |
title_sort |
healthy nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with metabolic syndrome - a sysdiet sub-study |
publisher |
Wiley |
publishDate |
2020 |
url |
https://hdl.handle.net/10642/8056 https://doi.org/10.1002/mnfr.201801405 |
long_lat |
ENVELOPE(-64.233,-64.233,-64.250,-64.250) |
geographic |
Norway Sigrid |
geographic_facet |
Norway Sigrid |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Molecular Nutrition & Food Research |
op_relation |
Molecular Nutrition and Food Research;Volume 63, Issue 13, July 2019 Myhrstad MC, De Mello VD, Dahlman I, Kolehmainen M, Paananen J, Rundblad A, Carlberg C, Olstad OK, Pihlajamaki J, Holven KB, Hermansen K, Dragsted LO, Gunnarsdottir I, Cloetens L, Storm MU, Åkesson B, Rosqvist F, Hukkanen J, Herzig K, Risérus U, Thorsdottir I, Poutanen KS, Savolainen MJ, Schwab U, Arner P, Uusitupa M, Ulven S. Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study. Molecular Nutrition & Food Research. 2019;63(13) urn:issn:1613-4125 urn:issn:1613-4133 https://hdl.handle.net/10642/8056 https://dx.doi.org/10.1002/mnfr.201801405 cristin:1713989 |
op_rights |
This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.1002/mnfr.201801405. This article is protected by copyright. All rights reserved. |
op_doi |
https://doi.org/10.1002/mnfr.201801405 |
container_title |
Molecular Nutrition & Food Research |
container_start_page |
1801405 |
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1766039783045332992 |
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fthsosloakersoda:oai:oda.oslomet.no:10642/8056 2023-05-15T16:49:39+02:00 Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study Myhrstad, Mari De Mello, Vanessa D. Dahlman, Ingrid Kolehmainen, Marjukka Paananen, Jussi Rundblad, Amanda Carlberg, Carsten Olstad, Ole Kristoffer Pihlajamaki, Jussi Holven, Kirsten Bjørklund Hermansen, Kjeld Dragsted, Lars O. Gunnarsdottir, Ingibjörg Cloetens, Lieselotte Storm, Matilda Ulmius Åkesson, Björn Rosqvist, Fredrik Hukkanen, Janne Herzig, Karl-Heinz Risérus, Ulf Thorsdottir, Inga Poutanen, Kaisa S. Savolainen, Markku J. Schwab, Ursula Arner, Peter Uusitupa, Matti Ulven, Stine Marie 2020-01-31T20:27:51Z application/pdf https://hdl.handle.net/10642/8056 https://doi.org/10.1002/mnfr.201801405 en eng Wiley Molecular Nutrition and Food Research;Volume 63, Issue 13, July 2019 Myhrstad MC, De Mello VD, Dahlman I, Kolehmainen M, Paananen J, Rundblad A, Carlberg C, Olstad OK, Pihlajamaki J, Holven KB, Hermansen K, Dragsted LO, Gunnarsdottir I, Cloetens L, Storm MU, Åkesson B, Rosqvist F, Hukkanen J, Herzig K, Risérus U, Thorsdottir I, Poutanen KS, Savolainen MJ, Schwab U, Arner P, Uusitupa M, Ulven S. Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome - A SYSDIET Sub-Study. Molecular Nutrition & Food Research. 2019;63(13) urn:issn:1613-4125 urn:issn:1613-4133 https://hdl.handle.net/10642/8056 https://dx.doi.org/10.1002/mnfr.201801405 cristin:1713989 This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.1002/mnfr.201801405. This article is protected by copyright. All rights reserved. Molecular Nutrition & Food Research Gene expressions Healthy nordic diets Metabolic syndromes Peripheralblood mononuclear cells Transcriptomes Journal article Peer reviewed 2020 fthsosloakersoda https://doi.org/10.1002/mnfr.201801405 2021-10-11T16:53:56Z Scope. To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results. Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p‐value < 0.05). Twenty‐five of these are significantly regulated (FDR q‐value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF‐κB, are downregulated in the healthy Nordic diet group. Conclusion. These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation. The project was funded by NordForsk Nordic Centre of Excellencein Food, Nutrition and Health project 070014 (SYSDIET [Systems Biologyin Controlled Dietary Interventions and Cohort Studies]) and further, OsloMetropolitan University-OsloMet (Norway), University of Oslo (Norway),Throne Holst Foundation (Norway), Academy of Finland, SwedishResearch Council, Svenska Diabetesf ̈orbundet, SRP Diabetes, FinnishDiabetes Research Foundation, Finnish Foundation for CardiovascularResearch, the Sigrid Juselius Foundation, EVO funding from KuopioUniversity Hospital (Finland), the Druvan Foundation, Sk ̊ane UniversityHospital, the Heart-Lung Foundation, Diabetesfonden and FoundationCerealia (Sweden), the Danish Obesity Research Centre (DanORC,www.danorc.dk), the Danish Council for Strategic Research (DairyHealth,BioFunCarb) (Denmark), the Agricultural Productivity Fund, and theResearch Fund of the University of Iceland (Iceland). acceptedVersion Article in Journal/Newspaper Iceland OsloMet (Oslo Metropolitan University): ODA (Open Digital Archive) Norway Sigrid ENVELOPE(-64.233,-64.233,-64.250,-64.250) Molecular Nutrition & Food Research 1801405 |