Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation
Artificial fertilization is increasingly used in aquaculture, mostly applying short-term cold stored milt. Large scale cryopreservation of milt could be valuable for increased flexibility and acceleration of breeding progress. The aim of this study was to assess viability, motility and ATP content o...
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Online Access: | https://hdl.handle.net/11250/2677668 https://doi.org/10.1016/j.theriogenology.2020.04.008 |
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fthsinnlandet:oai:brage.inn.no:11250/2677668 2024-03-03T08:42:48+00:00 Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation Kommisrud, Elisabeth Myromslien, Frøydis Deinboll Stenseth, Else-Berit Zeremichael, Teklu Tewoldebrhan Hofman, Nadine Grevle, Inger Synnøve Sunde, Jan 2020 application/pdf https://hdl.handle.net/11250/2677668 https://doi.org/10.1016/j.theriogenology.2020.04.008 eng eng Regionale forskningsfond Innlandet: 224814 urn:issn:0093-691X https://hdl.handle.net/11250/2677668 https://doi.org/10.1016/j.theriogenology.2020.04.008 cristin:1807156 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no 58-65 151 Theriogenology Lakseoppdrett Salmon breeding Inseminering kunstig / kunstig befruktning Insemination Artificial In vitro spermieanalyse In vitro sperm analyses Bevaring / preservering biologisk Preservation Biological VDP::Basale biofag: 470 VDP::Basic biosciences: 470 Peer reviewed Journal article 2020 fthsinnlandet https://doi.org/10.1016/j.theriogenology.2020.04.008 2024-02-02T12:42:29Z Artificial fertilization is increasingly used in aquaculture, mostly applying short-term cold stored milt. Large scale cryopreservation of milt could be valuable for increased flexibility and acceleration of breeding progress. The aim of this study was to assess viability, motility and ATP content of sperm from Atlantic salmon as a function of storage time, before and after cryopreservation. The objective was also to investigate whether in vitro parameters were associated with sperm fertilizing ability after cryopreservation. Milt from six mature Atlantic salmon males were collected twice, one week apart. The milt was stored undiluted at 5 °C in cell culture flasks for six days. Samples were taken on days 1, 3 and 6 of storage for cryopreservation. In total, 36 batches were diluted to a standardized sperm concentration of 2 × 109 spermatozoa/mL, filled into 0.5 mL French medium straws and cryopreserved. In vitro analyses were assessed on the same sample for the 72 combinations of male, collection week, days of storage and cold stored or frozen-thawed. Fertilization trials with cryopreserved milt were carried out for all 36 batches in triplicate for each combination of male, collection week, storage time and sperm:egg ratios of either 2 or 4 × 106 sperm per egg, respectively, totally 218 experimental units, including two egg controls. There was a significant influence of storage and collection week on sperm quality parameters, both cold stored and cryopreserved, and cryopreservation had a significant effect on all tested sperm quality parameters. High correlations for cold stored vs cryopreserved samples was demonstrated for ATP content (p < 0.00001), motility and velocity parameters (p < 0.001), but not for viability, straightness and linearity. The overall percentage of fertilization achieved was 73.9 ± 1.7%. Sperm collected in week 2 showed significantly lower fertility when cryopreserved after six days of storage than after 1 or 3 days for sperm to egg ratios of 2 × 106 (p < 0.005), while there was ... Article in Journal/Newspaper Atlantic salmon Salmo salar Høgskolen i Innlandet: Brage INN Theriogenology 151 58 65 |
institution |
Open Polar |
collection |
Høgskolen i Innlandet: Brage INN |
op_collection_id |
fthsinnlandet |
language |
English |
topic |
Lakseoppdrett Salmon breeding Inseminering kunstig / kunstig befruktning Insemination Artificial In vitro spermieanalyse In vitro sperm analyses Bevaring / preservering biologisk Preservation Biological VDP::Basale biofag: 470 VDP::Basic biosciences: 470 |
spellingShingle |
Lakseoppdrett Salmon breeding Inseminering kunstig / kunstig befruktning Insemination Artificial In vitro spermieanalyse In vitro sperm analyses Bevaring / preservering biologisk Preservation Biological VDP::Basale biofag: 470 VDP::Basic biosciences: 470 Kommisrud, Elisabeth Myromslien, Frøydis Deinboll Stenseth, Else-Berit Zeremichael, Teklu Tewoldebrhan Hofman, Nadine Grevle, Inger Synnøve Sunde, Jan Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation |
topic_facet |
Lakseoppdrett Salmon breeding Inseminering kunstig / kunstig befruktning Insemination Artificial In vitro spermieanalyse In vitro sperm analyses Bevaring / preservering biologisk Preservation Biological VDP::Basale biofag: 470 VDP::Basic biosciences: 470 |
description |
Artificial fertilization is increasingly used in aquaculture, mostly applying short-term cold stored milt. Large scale cryopreservation of milt could be valuable for increased flexibility and acceleration of breeding progress. The aim of this study was to assess viability, motility and ATP content of sperm from Atlantic salmon as a function of storage time, before and after cryopreservation. The objective was also to investigate whether in vitro parameters were associated with sperm fertilizing ability after cryopreservation. Milt from six mature Atlantic salmon males were collected twice, one week apart. The milt was stored undiluted at 5 °C in cell culture flasks for six days. Samples were taken on days 1, 3 and 6 of storage for cryopreservation. In total, 36 batches were diluted to a standardized sperm concentration of 2 × 109 spermatozoa/mL, filled into 0.5 mL French medium straws and cryopreserved. In vitro analyses were assessed on the same sample for the 72 combinations of male, collection week, days of storage and cold stored or frozen-thawed. Fertilization trials with cryopreserved milt were carried out for all 36 batches in triplicate for each combination of male, collection week, storage time and sperm:egg ratios of either 2 or 4 × 106 sperm per egg, respectively, totally 218 experimental units, including two egg controls. There was a significant influence of storage and collection week on sperm quality parameters, both cold stored and cryopreserved, and cryopreservation had a significant effect on all tested sperm quality parameters. High correlations for cold stored vs cryopreserved samples was demonstrated for ATP content (p < 0.00001), motility and velocity parameters (p < 0.001), but not for viability, straightness and linearity. The overall percentage of fertilization achieved was 73.9 ± 1.7%. Sperm collected in week 2 showed significantly lower fertility when cryopreserved after six days of storage than after 1 or 3 days for sperm to egg ratios of 2 × 106 (p < 0.005), while there was ... |
format |
Article in Journal/Newspaper |
author |
Kommisrud, Elisabeth Myromslien, Frøydis Deinboll Stenseth, Else-Berit Zeremichael, Teklu Tewoldebrhan Hofman, Nadine Grevle, Inger Synnøve Sunde, Jan |
author_facet |
Kommisrud, Elisabeth Myromslien, Frøydis Deinboll Stenseth, Else-Berit Zeremichael, Teklu Tewoldebrhan Hofman, Nadine Grevle, Inger Synnøve Sunde, Jan |
author_sort |
Kommisrud, Elisabeth |
title |
Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation |
title_short |
Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation |
title_full |
Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation |
title_fullStr |
Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation |
title_full_unstemmed |
Viability, motility, ATP content and fertilizing potential of sperm from Atlantic salmon (Salmo salar L.) in milt stored before cryopreservation |
title_sort |
viability, motility, atp content and fertilizing potential of sperm from atlantic salmon (salmo salar l.) in milt stored before cryopreservation |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2677668 https://doi.org/10.1016/j.theriogenology.2020.04.008 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
58-65 151 Theriogenology |
op_relation |
Regionale forskningsfond Innlandet: 224814 urn:issn:0093-691X https://hdl.handle.net/11250/2677668 https://doi.org/10.1016/j.theriogenology.2020.04.008 cristin:1807156 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no |
op_doi |
https://doi.org/10.1016/j.theriogenology.2020.04.008 |
container_title |
Theriogenology |
container_volume |
151 |
container_start_page |
58 |
op_container_end_page |
65 |
_version_ |
1792498261165080576 |