Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau
The French–Italian Concordia Research Station, situated on the Antarctic Polar Plateau at an elevation of 3233 m above sea level, offers a unique opportunity to study the presence and variation of microbes introduced by abiotic or biotic vectors and, consequently, appraise the amplitude of human imp...
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Hochschule Bonn-Rhein-Sieg
2023
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Online Access: | https://pub.h-brs.de/frontdoor/index/index/docId/7525 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-75251 https://pub.h-brs.de/files/7525/biology-12-01193-v2.pdf |
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fthsbonnrhsieg:oai:pub.h-brs.de:7525 2024-04-28T08:02:16+00:00 Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau Stoppiello, Gerardo A. Coleine, Claudia Moeller, Ralf Ripa, Caterina Billi, Daniela Selbmann, Laura 2023-08-31 application/pdf https://pub.h-brs.de/frontdoor/index/index/docId/7525 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-75251 https://pub.h-brs.de/files/7525/biology-12-01193-v2.pdf eng eng Hochschule Bonn-Rhein-Sieg info:eu-repo/semantics/altIdentifier/doi/10.3390/biology12091193 info:eu-repo/grantAgreement/EC/H2020/654208 https://pub.h-brs.de/frontdoor/index/index/docId/7525 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-75251 https://pub.h-brs.de/files/7525/biology-12-01193-v2.pdf info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ Biology ISSN 2079-7737 Biology 2023, 12(9), 1193 ddc:579 article doc-type:article publishedVersion 2023 fthsbonnrhsieg https://doi.org/10.3390/biology12091193 2024-04-11T00:01:44Z The French–Italian Concordia Research Station, situated on the Antarctic Polar Plateau at an elevation of 3233 m above sea level, offers a unique opportunity to study the presence and variation of microbes introduced by abiotic or biotic vectors and, consequently, appraise the amplitude of human impact in such a pristine environment. This research built upon a previous work, which explored microbial diversity in the surface snow surrounding the Concordia Research Station. While that study successfully characterized the bacterial assemblage, detecting fungal diversity was hampered by the low DNA content. To address this knowledge gap, in the present study, we optimized the sampling by increasing ice/snow collected to leverage the final DNA yield. The V4 variable region of the 16S rDNA and Internal Transcribed Spacer (ITS1) rDNA was used to evaluate bacterial and fungal diversity. From the sequencing, we obtained 3,352,661 and 4,433,595 reads clustered in 930 and 3182 amplicon sequence variants (ASVs) for fungi and bacteria, respectively. Amplicon sequencing revealed a predominance of Basidiomycota (49%) and Ascomycota (42%) in the fungal component; Bacteroidota (65.8%) is the main representative among the bacterial phyla. Basidiomycetes are almost exclusively represented by yeast-like fungi. Our findings provide the first comprehensive overview of both fungal and bacterial diversity in the Antarctic Polar Plateau’s surface snow/ice near Concordia Station and to identify seasonality as the main driver of microbial diversity; we also detected the most sensitive microorganisms to these factors, which could serve as indicators of human impact in this pristine environment and aid in planetary protection for future exploration missions. Article in Journal/Newspaper Antarc* Antarctic pub H-BRS - Publication Server of Bonn-Rhein-Sieg University of Applied Sciences Biology 12 9 1193 |
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pub H-BRS - Publication Server of Bonn-Rhein-Sieg University of Applied Sciences |
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fthsbonnrhsieg |
language |
English |
topic |
ddc:579 |
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ddc:579 Stoppiello, Gerardo A. Coleine, Claudia Moeller, Ralf Ripa, Caterina Billi, Daniela Selbmann, Laura Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau |
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ddc:579 |
description |
The French–Italian Concordia Research Station, situated on the Antarctic Polar Plateau at an elevation of 3233 m above sea level, offers a unique opportunity to study the presence and variation of microbes introduced by abiotic or biotic vectors and, consequently, appraise the amplitude of human impact in such a pristine environment. This research built upon a previous work, which explored microbial diversity in the surface snow surrounding the Concordia Research Station. While that study successfully characterized the bacterial assemblage, detecting fungal diversity was hampered by the low DNA content. To address this knowledge gap, in the present study, we optimized the sampling by increasing ice/snow collected to leverage the final DNA yield. The V4 variable region of the 16S rDNA and Internal Transcribed Spacer (ITS1) rDNA was used to evaluate bacterial and fungal diversity. From the sequencing, we obtained 3,352,661 and 4,433,595 reads clustered in 930 and 3182 amplicon sequence variants (ASVs) for fungi and bacteria, respectively. Amplicon sequencing revealed a predominance of Basidiomycota (49%) and Ascomycota (42%) in the fungal component; Bacteroidota (65.8%) is the main representative among the bacterial phyla. Basidiomycetes are almost exclusively represented by yeast-like fungi. Our findings provide the first comprehensive overview of both fungal and bacterial diversity in the Antarctic Polar Plateau’s surface snow/ice near Concordia Station and to identify seasonality as the main driver of microbial diversity; we also detected the most sensitive microorganisms to these factors, which could serve as indicators of human impact in this pristine environment and aid in planetary protection for future exploration missions. |
format |
Article in Journal/Newspaper |
author |
Stoppiello, Gerardo A. Coleine, Claudia Moeller, Ralf Ripa, Caterina Billi, Daniela Selbmann, Laura |
author_facet |
Stoppiello, Gerardo A. Coleine, Claudia Moeller, Ralf Ripa, Caterina Billi, Daniela Selbmann, Laura |
author_sort |
Stoppiello, Gerardo A. |
title |
Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau |
title_short |
Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau |
title_full |
Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau |
title_fullStr |
Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau |
title_full_unstemmed |
Seasonality Is the Main Determinant of Microbial Diversity Associated to Snow/Ice around Concordia Station on the Antarctic Polar Plateau |
title_sort |
seasonality is the main determinant of microbial diversity associated to snow/ice around concordia station on the antarctic polar plateau |
publisher |
Hochschule Bonn-Rhein-Sieg |
publishDate |
2023 |
url |
https://pub.h-brs.de/frontdoor/index/index/docId/7525 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-75251 https://pub.h-brs.de/files/7525/biology-12-01193-v2.pdf |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Biology ISSN 2079-7737 Biology 2023, 12(9), 1193 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/biology12091193 info:eu-repo/grantAgreement/EC/H2020/654208 https://pub.h-brs.de/frontdoor/index/index/docId/7525 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-75251 https://pub.h-brs.de/files/7525/biology-12-01193-v2.pdf |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biology12091193 |
container_title |
Biology |
container_volume |
12 |
container_issue |
9 |
container_start_page |
1193 |
_version_ |
1797573679173861376 |