Snow Surface Microbial Diversity at the Detection Limit within the Vicinity of the Concordia Station, Antarctica
The Concordia Research Station provides a unique location for preparatory activities for future human journey to Mars, to explore microbial diversity at subzero temperatures, and monitor the dissemination of human-associated microorganisms within the pristine surrounding environment. Amplicon sequen...
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2023
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fthsbonnrhsieg:oai:pub.h-brs.de:6565 2024-04-28T08:00:33+00:00 Snow Surface Microbial Diversity at the Detection Limit within the Vicinity of the Concordia Station, Antarctica Napoli, Alessandro Coleine, Claudia Ulrich, Nikea J. Moeller, Ralf Billi, Daniela Selbmann, Laura 2023 application/pdf https://pub.h-brs.de/frontdoor/index/index/docId/6565 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-65655 https://pub.h-brs.de/files/6565/life-13-00113.pdf eng eng Hochschule Bonn-Rhein-Sieg info:eu-repo/altidentifier/doi/10.3390/life13010113 info:eu-repo/grantAgreement/EC/H2020/654208 https://pub.h-brs.de/frontdoor/index/index/docId/6565 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-65655 https://pub.h-brs.de/files/6565/life-13-00113.pdf info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ Life ISSN 2075-1729 Life 2023, 13(1), 113 ddc:570 article doc-type:article publishedVersion 2023 fthsbonnrhsieg 2024-04-11T00:01:44Z The Concordia Research Station provides a unique location for preparatory activities for future human journey to Mars, to explore microbial diversity at subzero temperatures, and monitor the dissemination of human-associated microorganisms within the pristine surrounding environment. Amplicon sequencing was leveraged to investigate the microbial diversity of surface snow samples collected monthly over a two-year period, at three distances from the Station (10, 500, and 1000 m). Even when the extracted total DNA was below the detection limit, 16S rRNA gene sequencing was successfully performed on all samples, while 18S rRNA was amplified on 19 samples out of 51. No significant relationships were observed between microbial diversity and seasonality (summer or winter) or distance from the Concordia base. This suggested that if present, the anthropogenic impact should have been below the detectable limit. While harboring low microbial diversity, the surface snow samples were characterized by heterogeneous microbiomes. Ultimately, our study corroborated the use of DNA sequencing-based techniques for revealing microbial presence in remote and hostile environments, with implications for Planetary Protection during space missions and for life-detection in astrobiology relevant targets. Article in Journal/Newspaper Antarc* Antarctica pub H-BRS - Publication Server of Bonn-Rhein-Sieg University of Applied Sciences |
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pub H-BRS - Publication Server of Bonn-Rhein-Sieg University of Applied Sciences |
op_collection_id |
fthsbonnrhsieg |
language |
English |
topic |
ddc:570 |
spellingShingle |
ddc:570 Napoli, Alessandro Coleine, Claudia Ulrich, Nikea J. Moeller, Ralf Billi, Daniela Selbmann, Laura Snow Surface Microbial Diversity at the Detection Limit within the Vicinity of the Concordia Station, Antarctica |
topic_facet |
ddc:570 |
description |
The Concordia Research Station provides a unique location for preparatory activities for future human journey to Mars, to explore microbial diversity at subzero temperatures, and monitor the dissemination of human-associated microorganisms within the pristine surrounding environment. Amplicon sequencing was leveraged to investigate the microbial diversity of surface snow samples collected monthly over a two-year period, at three distances from the Station (10, 500, and 1000 m). Even when the extracted total DNA was below the detection limit, 16S rRNA gene sequencing was successfully performed on all samples, while 18S rRNA was amplified on 19 samples out of 51. No significant relationships were observed between microbial diversity and seasonality (summer or winter) or distance from the Concordia base. This suggested that if present, the anthropogenic impact should have been below the detectable limit. While harboring low microbial diversity, the surface snow samples were characterized by heterogeneous microbiomes. Ultimately, our study corroborated the use of DNA sequencing-based techniques for revealing microbial presence in remote and hostile environments, with implications for Planetary Protection during space missions and for life-detection in astrobiology relevant targets. |
format |
Article in Journal/Newspaper |
author |
Napoli, Alessandro Coleine, Claudia Ulrich, Nikea J. Moeller, Ralf Billi, Daniela Selbmann, Laura |
author_facet |
Napoli, Alessandro Coleine, Claudia Ulrich, Nikea J. Moeller, Ralf Billi, Daniela Selbmann, Laura |
author_sort |
Napoli, Alessandro |
title |
Snow Surface Microbial Diversity at the Detection Limit within the Vicinity of the Concordia Station, Antarctica |
title_short |
Snow Surface Microbial Diversity at the Detection Limit within the Vicinity of the Concordia Station, Antarctica |
title_full |
Snow Surface Microbial Diversity at the Detection Limit within the Vicinity of the Concordia Station, Antarctica |
title_fullStr |
Snow Surface Microbial Diversity at the Detection Limit within the Vicinity of the Concordia Station, Antarctica |
title_full_unstemmed |
Snow Surface Microbial Diversity at the Detection Limit within the Vicinity of the Concordia Station, Antarctica |
title_sort |
snow surface microbial diversity at the detection limit within the vicinity of the concordia station, antarctica |
publisher |
Hochschule Bonn-Rhein-Sieg |
publishDate |
2023 |
url |
https://pub.h-brs.de/frontdoor/index/index/docId/6565 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-65655 https://pub.h-brs.de/files/6565/life-13-00113.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Life ISSN 2075-1729 Life 2023, 13(1), 113 |
op_relation |
info:eu-repo/altidentifier/doi/10.3390/life13010113 info:eu-repo/grantAgreement/EC/H2020/654208 https://pub.h-brs.de/frontdoor/index/index/docId/6565 https://nbn-resolving.org/urn:nbn:de:hbz:1044-opus-65655 https://pub.h-brs.de/files/6565/life-13-00113.pdf |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
_version_ |
1797572719416442880 |