Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology
The conditions, including mass ratio of PEG4000 to lipase, pH, and mass ratio of diatomites to lipase, for immobilization of Candida antarctica lipase with PEG non-covalent modification were optimized by means of the response surface methodology (RSM). The immobilized lipase specific activity in the...
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Croatian Society of Chemical Engineers; kui@zg.t-com.hr
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fthrcak:oai:hrcak.srce.hr:55034 2023-05-15T14:04:33+02:00 Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology Liu, J.-H.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China; liujh@qust.edu.cn Zhang, Y.-Y.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Xia, Y.-M.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Su, F.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China 2010-07-01 pdf http://hrcak.srce.hr/55034 http://hrcak.srce.hr/file/83742 en eng Croatian Society of Chemical Engineers; kui@zg.t-com.hr http://hrcak.srce.hr/55034 http://hrcak.srce.hr/file/83742 Chemical & Biochemical Engineering Quarterly (cabeq@pbf.hr); Vol.24 No.2 Response surface methodology Candida antarctica lipase immobilization optimization PEG text 2010 fthrcak 2012-10-27T14:55:00Z The conditions, including mass ratio of PEG4000 to lipase, pH, and mass ratio of diatomites to lipase, for immobilization of Candida antarctica lipase with PEG non-covalent modification were optimized by means of the response surface methodology (RSM). The immobilized lipase specific activity in the reaction of transesterification was selected as the response value. A mathematical model was developed to investigate the influences of various immobilization parameters and to predict the optimum immobilization conditions for lipase. The maximal specific activity was predicted to occur when PEG4000: lipase (w/w) was 7.61, diatomites: lipase (w/w), 9.92 and pH, 7.52, respectively. A repeat immobilization experiment of lipase was carried out under the optimized conditions for the verification of optimization. The maximal specific activity obtained experimentally was 56.11 U mg–1 pr., which was significantly higher than that obtained under unoptimized conditions. Text Antarc* Antarctica Hrčak - Portal of scientific journals of Croatia |
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Hrčak - Portal of scientific journals of Croatia |
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Response surface methodology Candida antarctica lipase immobilization optimization PEG |
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Response surface methodology Candida antarctica lipase immobilization optimization PEG Liu, J.-H.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China; liujh@qust.edu.cn Zhang, Y.-Y.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Xia, Y.-M.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Su, F.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology |
topic_facet |
Response surface methodology Candida antarctica lipase immobilization optimization PEG |
description |
The conditions, including mass ratio of PEG4000 to lipase, pH, and mass ratio of diatomites to lipase, for immobilization of Candida antarctica lipase with PEG non-covalent modification were optimized by means of the response surface methodology (RSM). The immobilized lipase specific activity in the reaction of transesterification was selected as the response value. A mathematical model was developed to investigate the influences of various immobilization parameters and to predict the optimum immobilization conditions for lipase. The maximal specific activity was predicted to occur when PEG4000: lipase (w/w) was 7.61, diatomites: lipase (w/w), 9.92 and pH, 7.52, respectively. A repeat immobilization experiment of lipase was carried out under the optimized conditions for the verification of optimization. The maximal specific activity obtained experimentally was 56.11 U mg–1 pr., which was significantly higher than that obtained under unoptimized conditions. |
format |
Text |
author |
Liu, J.-H.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China; liujh@qust.edu.cn Zhang, Y.-Y.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Xia, Y.-M.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Su, F.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China |
author_facet |
Liu, J.-H.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China; liujh@qust.edu.cn Zhang, Y.-Y.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Xia, Y.-M.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China Su, F.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China |
author_sort |
Liu, J.-H.; Department of Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China; liujh@qust.edu.cn |
title |
Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology |
title_short |
Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology |
title_full |
Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology |
title_fullStr |
Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology |
title_full_unstemmed |
Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology |
title_sort |
optimization of immobilization conditions of candida antarctica lipase based on response surface methodology |
publisher |
Croatian Society of Chemical Engineers; kui@zg.t-com.hr |
publishDate |
2010 |
url |
http://hrcak.srce.hr/55034 http://hrcak.srce.hr/file/83742 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Chemical & Biochemical Engineering Quarterly (cabeq@pbf.hr); Vol.24 No.2 |
op_relation |
http://hrcak.srce.hr/55034 http://hrcak.srce.hr/file/83742 |
_version_ |
1766275719233536000 |