Characterization of Collagen from Sakhalin Taimen Skin as Useful Biomass

• Published in: Food Technology and Biotechnology
• Main Authors: Nagai, Takeshi, Saito, Masataka, Tanoue, Yasuhiro, Kai, Norihisa, Suzuki, Nobutaka
• Format: Article in Journal/Newspaper
• Language: English; Croatian
• Published: 2020
• Subjects: Sakhalin taimen skin; useful biomass; collagen; succinylation; improvement of functional property; koža sahalinske mladice; korisna biomasa; kolagen; sukcinilacija; poboljšanje funkcionalnih svojstava; Sakhalin
• Online Access: https://hrcak.srce.hr/251261; https://hrcak.srce.hr/file/372672; https://hrcak.srce.hr/file/372684

Animal collagen has been widely utilized in foods, cosmetics and biomedical fields. The non-edible portion, such as fish skin and bones, are obtained during cooking. Most of them are currently discarded as wastes, although the nutritional value of the skin and bones is high. The non-edible portion needs to be reused in order to reduce environmental impact, as it is one of the sources of environmental pollution. Collagen was prepared by cold acetone treatment from Sakhalin taimen skin as a waste produced during cooking. Next, the colour, SDS-polyacrylamide gel electrophoresis, ultraviolet absorption, subunit composition, amino acid composition, denaturation temperature and attenuated total reflectance-Fourier transform infrared spectroscopy analyses were conducted to explore the properties of the collagen. Lastly, we attempted to improve the functional properties of the collagen for future applications using chemical modification technique (succinylation). Cold acetone treatment easily removed the fats and pigments from the skin. The odourless and pure white collagen was obtained with high yield. The α3 chain did not exist in the collagen. Sakhalin taimen skin collagen had rich α-helix and low β-sheet structures. Succinylation caused the secondary structural changes of the collagen molecule. Moreover, it made it possible not only to increase the viscosity of the collagen solution but also to improve the solubility of the collagen under the physiological conditions around pH=6. This finding is the first report on the absence of the α3 chain from salmonid fish skin collagens. The succinylated collagen from Sakhalin taimen skins as useful biomass has potential to utilize in foods, cosmetics and related industries. Pozadina istraživanja. Kolagen životinjskog podrijetla uvelike se koristi u proizvodnji hrane, kozmetičkih i biomedicinskih proizvoda. Tijekom kuhanja se nejestivi dijelovi riba, kao što su kosti i koža, uglavnom odbacuju kao smeće, iako imaju veliku hranjivu vrijednost. S obzirom na to da taj otpad predstavlja izvor zagađenja, potrebno ga je iskoristiti da bi se smanjio njegov negativan utjecaj na okoliš. Eksperimentalni pristup. Kolagen je izdvojen iz kože sahalinske mladice, dobivene kao otpad pri kuhanju, nakon obrade ohlađenim acetonom. Zatim su ispitana sljedeća svojstva kolagena: boja, čistoća (pomoću poliakrilamid gel elektroforeze u prisutnosti natrijevog dodecil sulfata), apsorpcija ultraljubičastih zraka, sastav podjedinica, aminokiselinski sastav, temperatura denaturacije i struktura (pomoću prigušene totalne refleksije i infracrvene spektroskopije s Fourierovom transformacijom). Naposljetku smo kemijskom modifikacijom (sukcinilacijom) nastojali poboljšati funkcionalna svojstva kolagena za njegovu daljnju primjenu. Rezultati i zaključci. Obradom ohlađenim acetonom iz riblje kože su uspješno uklonjene masti i pigmenti. Dobiven je veliki prinos čistog bijelog kolagena bez mirisa, u kojem nije pronađen niti jedan α3-lanac. Kolagen iz kože sahalinske mladice sadržavao je velik udjel α-uzvojnica, a malen udjel β-ploča. Sukcinilacija je uzrokovala promjenu sekundarne strukture molekule kolagena. Osim toga, povećala se viskoznost otopine kolagena i poboljšala topljivost kolagena pri fiziološkim uvjetima pH-vrijednosti od otprilike 6. Novina i znanstveni doprinos. Ovi rezultati po prvi put pokazuju da kolagen iz kože ribe iz porodice salmonida ne sadržava α3-lance. Sukcinilirani kolagen iz kože sahalinske mladice korisna je biomasa, koja se može primijeniti u prehrambenoj i kozmetičkoj industriji, te srodnim industrijama.