Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents
This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as glycer...
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Croatian Society of Chemical Engineers
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fthrcak:oai:hrcak.srce.hr:206732 2023-05-15T14:15:37+02:00 Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents Elgharbawy, A. A. Hayyan, A. Hayyan, M. Rashid, S. N. Nor, M. R. M. Zulkifli, M. Y. Alias, Y. Mirghani, M. E. S. 2018-10-13 application/pdf https://hrcak.srce.hr/206732 https://hrcak.srce.hr/file/303781 eng eng Croatian Society of Chemical Engineers info:eu-repo/semantics/altIdentifier/doi/10.15255/CABEQ.2018.1335 https://hrcak.srce.hr/206732 info:eu-repo/semantics/openAccess Journal "Chemical and Biochemical Engineering Quarterly" is an Open Access journal at the highest possible level meaning that all content is immediately and freely available to anyone, anywhere, to be downloaded, printed, distributed, read, reused, self archived, and remixed (including commercially) without restriction, as long as the author and the original source are properly attributed according to the Creative Commons Attribution 4.0 International License (CC BY). The author(s) hold the copyright and retain publishing rights without restrictions. CC BY (Creative Commons Attribution) is the most accommodating of public copyright licenses as defined by Creative Commons, a nonprofit organization that provides legal tools for sharing and use of creative works and research. The CC BY license is recommended for maximum dissemination and use of licensed materials. All content published in Kemija u industriji is available under CC BY, meaning anyone is free to use and reuse the content provided the original source and authors are credited. The copyright is held and retained. The author(s) hold the copyright without restrictions CC BY is the appropriate license for publicly funded research; it maximizes the potential for both economic and scholarly impact, protects the rights of authors and strengthens the long-standing tradition of appropriate attribution and credit for scholarship. Journal does not charge neither article processing charges (APCs) nor article submission charges. Self-archiving policy Sherpa/ROMEO blue route since 2013. All published manuscripts are licensed under a Creative Commons Attribution 4.0 International License. CC-BY Chemical and Biochemical Engineering Quarterly ISSN 0352-9568 (Print) ISSN 1846-5153 (Online) Volume 32 Issue 3 biotransformation biotechnology ionic liquids choline chloride p-nitrophenyl palmitate lipase B Candida antartica text info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2018 fthrcak https://doi.org/10.15255/CABEQ.2018.1335 2018-10-17T23:00:36Z This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as glycerol and malonic acid and sugars (glucose, fructose and sucrose). The hydrolysis of p-nitrophenyl palmitate by six different lipases: lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS, from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. The results showed that NADES3 prepared from ChCl/sucrose was the most promising solvent as it enhanced the activities of both CALB and lipase from porcine pancreas to 355 % and 345 %. The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km) of lipases in the 40 % of (NADES3) and compared with the aqueous form. The trend achieved by NADES may be a promising approach for applications and further perspectives as genuinely green industrial solvents. Article in Journal/Newspaper antartic* Hrčak - Portal of scientific journals of Croatia Rugosa ENVELOPE(-61.250,-61.250,-62.633,-62.633) Chemical and Biochemical Engineering Quarterly 32 3 359 370 |
institution |
Open Polar |
collection |
Hrčak - Portal of scientific journals of Croatia |
op_collection_id |
fthrcak |
language |
English |
topic |
biotransformation biotechnology ionic liquids choline chloride p-nitrophenyl palmitate lipase B Candida antartica |
spellingShingle |
biotransformation biotechnology ionic liquids choline chloride p-nitrophenyl palmitate lipase B Candida antartica Elgharbawy, A. A. Hayyan, A. Hayyan, M. Rashid, S. N. Nor, M. R. M. Zulkifli, M. Y. Alias, Y. Mirghani, M. E. S. Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents |
topic_facet |
biotransformation biotechnology ionic liquids choline chloride p-nitrophenyl palmitate lipase B Candida antartica |
description |
This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as glycerol and malonic acid and sugars (glucose, fructose and sucrose). The hydrolysis of p-nitrophenyl palmitate by six different lipases: lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS, from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. The results showed that NADES3 prepared from ChCl/sucrose was the most promising solvent as it enhanced the activities of both CALB and lipase from porcine pancreas to 355 % and 345 %. The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km) of lipases in the 40 % of (NADES3) and compared with the aqueous form. The trend achieved by NADES may be a promising approach for applications and further perspectives as genuinely green industrial solvents. |
format |
Article in Journal/Newspaper |
author |
Elgharbawy, A. A. Hayyan, A. Hayyan, M. Rashid, S. N. Nor, M. R. M. Zulkifli, M. Y. Alias, Y. Mirghani, M. E. S. |
author_facet |
Elgharbawy, A. A. Hayyan, A. Hayyan, M. Rashid, S. N. Nor, M. R. M. Zulkifli, M. Y. Alias, Y. Mirghani, M. E. S. |
author_sort |
Elgharbawy, A. A. |
title |
Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents |
title_short |
Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents |
title_full |
Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents |
title_fullStr |
Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents |
title_full_unstemmed |
Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents |
title_sort |
shedding light on lipase stability in natural deep eutectic solvents |
publisher |
Croatian Society of Chemical Engineers |
publishDate |
2018 |
url |
https://hrcak.srce.hr/206732 https://hrcak.srce.hr/file/303781 |
long_lat |
ENVELOPE(-61.250,-61.250,-62.633,-62.633) |
geographic |
Rugosa |
geographic_facet |
Rugosa |
genre |
antartic* |
genre_facet |
antartic* |
op_source |
Chemical and Biochemical Engineering Quarterly ISSN 0352-9568 (Print) ISSN 1846-5153 (Online) Volume 32 Issue 3 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.15255/CABEQ.2018.1335 https://hrcak.srce.hr/206732 |
op_rights |
info:eu-repo/semantics/openAccess Journal "Chemical and Biochemical Engineering Quarterly" is an Open Access journal at the highest possible level meaning that all content is immediately and freely available to anyone, anywhere, to be downloaded, printed, distributed, read, reused, self archived, and remixed (including commercially) without restriction, as long as the author and the original source are properly attributed according to the Creative Commons Attribution 4.0 International License (CC BY). The author(s) hold the copyright and retain publishing rights without restrictions. CC BY (Creative Commons Attribution) is the most accommodating of public copyright licenses as defined by Creative Commons, a nonprofit organization that provides legal tools for sharing and use of creative works and research. The CC BY license is recommended for maximum dissemination and use of licensed materials. All content published in Kemija u industriji is available under CC BY, meaning anyone is free to use and reuse the content provided the original source and authors are credited. The copyright is held and retained. The author(s) hold the copyright without restrictions CC BY is the appropriate license for publicly funded research; it maximizes the potential for both economic and scholarly impact, protects the rights of authors and strengthens the long-standing tradition of appropriate attribution and credit for scholarship. Journal does not charge neither article processing charges (APCs) nor article submission charges. Self-archiving policy Sherpa/ROMEO blue route since 2013. All published manuscripts are licensed under a Creative Commons Attribution 4.0 International License. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.15255/CABEQ.2018.1335 |
container_title |
Chemical and Biochemical Engineering Quarterly |
container_volume |
32 |
container_issue |
3 |
container_start_page |
359 |
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370 |
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1766287949800931328 |