Proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas
© 2014 American Chemical Society. The Pacific oyster Crassostrea gigas is one of the dominant sessile inhabitants of the estuarine intertidal zone, which is a physically harsh environment due to the presence of a number of stressors. Oysters have adapted to highly dynamic and stressful environments,...
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fthongkongbapti:oai:repository.hkbu.edu.hk:hkbu_staff_publication-7000 2023-05-15T15:58:28+02:00 Proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas Zhang, Yang Sun, Jin Mu, Huawei Li, Jun Zhang, Yuehuan Xu, Fengjiao Xiang, Zhiming Qian, Pei-Yuan Qiu, Jianwen Yu, Ziniu 2014-01-01T08:00:00Z https://repository.hkbu.edu.hk/hkbu_staff_publication/5986 English eng HKBU Institutional Repository https://repository.hkbu.edu.hk/hkbu_staff_publication/5986 HKBU Staff Publication aerial exposure Crassostrea gills iTRAQ oyster salinity temperature Department of Biology text 2014 fthongkongbapti 2021-08-06T11:03:31Z © 2014 American Chemical Society. The Pacific oyster Crassostrea gigas is one of the dominant sessile inhabitants of the estuarine intertidal zone, which is a physically harsh environment due to the presence of a number of stressors. Oysters have adapted to highly dynamic and stressful environments, but the molecular mechanisms underlying such stress adaptation are largely unknown. In the present study, we examined the proteomic responses in the gills of C. gigas exposed to three stressors (high temperature, low salinity, and aerial exposure) they often encounter in the field. We quantitatively compared the gill proteome profiles using iTRAQ-coupled 2-D LC-MS/MS. There were 3165 identified proteins among which 2379 proteins could be quantified. Heat shock, hyposalinity, and aerial exposure resulted in 50, 15, and 33 differentially expressed gill proteins, respectively. Venn diagram analysis revealed substantial different responses to the three stressors. Only xanthine dehydrogenase/oxidase showed a similar expression pattern across the three stress treatments, suggesting that reduction of ROS accumulation may be a conserved response to these stressors. Heat shock caused significant overexpression of molecular chaperones and production of S-adenosyl-l-methionine, indicating their crucial protective roles against protein denature. In addition, heat shock also activated immune responses, Ca2+ binding protein expression. By contrast, hyposalinity and aerial exposure resulted in the up-regulation of 3-demethylubiquinone-9 3-methyltransferase, indicating that increase in ubiquinone synthesis may contribute to withstanding both the osmotic and desiccation stress. Strikingly, the majority of desiccation-responsive proteins, including those involved in metabolism, ion transportation, immune responses, DNA duplication, and protein synthesis, were down-regulated, indicating conservation of energy as an important strategy to cope with desiccation stress. There was a high consistency between the expression levels determined by iTRAQ and Western blotting, highlighting the high reproducibility of our proteomic approach and its great value in revealing molecular mechanisms of stress responses. Text Crassostrea gigas Pacific oyster Hong Kong Baptist University: HKBU Institutional Repository Pacific Venn ENVELOPE(9.281,9.281,63.350,63.350) |
institution |
Open Polar |
collection |
Hong Kong Baptist University: HKBU Institutional Repository |
op_collection_id |
fthongkongbapti |
language |
English |
topic |
aerial exposure Crassostrea gills iTRAQ oyster salinity temperature Department of Biology |
spellingShingle |
aerial exposure Crassostrea gills iTRAQ oyster salinity temperature Department of Biology Zhang, Yang Sun, Jin Mu, Huawei Li, Jun Zhang, Yuehuan Xu, Fengjiao Xiang, Zhiming Qian, Pei-Yuan Qiu, Jianwen Yu, Ziniu Proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas |
topic_facet |
aerial exposure Crassostrea gills iTRAQ oyster salinity temperature Department of Biology |
description |
© 2014 American Chemical Society. The Pacific oyster Crassostrea gigas is one of the dominant sessile inhabitants of the estuarine intertidal zone, which is a physically harsh environment due to the presence of a number of stressors. Oysters have adapted to highly dynamic and stressful environments, but the molecular mechanisms underlying such stress adaptation are largely unknown. In the present study, we examined the proteomic responses in the gills of C. gigas exposed to three stressors (high temperature, low salinity, and aerial exposure) they often encounter in the field. We quantitatively compared the gill proteome profiles using iTRAQ-coupled 2-D LC-MS/MS. There were 3165 identified proteins among which 2379 proteins could be quantified. Heat shock, hyposalinity, and aerial exposure resulted in 50, 15, and 33 differentially expressed gill proteins, respectively. Venn diagram analysis revealed substantial different responses to the three stressors. Only xanthine dehydrogenase/oxidase showed a similar expression pattern across the three stress treatments, suggesting that reduction of ROS accumulation may be a conserved response to these stressors. Heat shock caused significant overexpression of molecular chaperones and production of S-adenosyl-l-methionine, indicating their crucial protective roles against protein denature. In addition, heat shock also activated immune responses, Ca2+ binding protein expression. By contrast, hyposalinity and aerial exposure resulted in the up-regulation of 3-demethylubiquinone-9 3-methyltransferase, indicating that increase in ubiquinone synthesis may contribute to withstanding both the osmotic and desiccation stress. Strikingly, the majority of desiccation-responsive proteins, including those involved in metabolism, ion transportation, immune responses, DNA duplication, and protein synthesis, were down-regulated, indicating conservation of energy as an important strategy to cope with desiccation stress. There was a high consistency between the expression levels determined by iTRAQ and Western blotting, highlighting the high reproducibility of our proteomic approach and its great value in revealing molecular mechanisms of stress responses. |
format |
Text |
author |
Zhang, Yang Sun, Jin Mu, Huawei Li, Jun Zhang, Yuehuan Xu, Fengjiao Xiang, Zhiming Qian, Pei-Yuan Qiu, Jianwen Yu, Ziniu |
author_facet |
Zhang, Yang Sun, Jin Mu, Huawei Li, Jun Zhang, Yuehuan Xu, Fengjiao Xiang, Zhiming Qian, Pei-Yuan Qiu, Jianwen Yu, Ziniu |
author_sort |
Zhang, Yang |
title |
Proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas |
title_short |
Proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas |
title_full |
Proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas |
title_fullStr |
Proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas |
title_full_unstemmed |
Proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas |
title_sort |
proteomic basis of stress responses in the gills of the pacific oyster crassostrea gigas |
publisher |
HKBU Institutional Repository |
publishDate |
2014 |
url |
https://repository.hkbu.edu.hk/hkbu_staff_publication/5986 |
long_lat |
ENVELOPE(9.281,9.281,63.350,63.350) |
geographic |
Pacific Venn |
geographic_facet |
Pacific Venn |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
HKBU Staff Publication |
op_relation |
https://repository.hkbu.edu.hk/hkbu_staff_publication/5986 |
_version_ |
1766394216682881024 |