Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears
Robust estimates of demographic parameters are critical for effective wildlife conservation and management but are difficult to obtain for elusive species. We estimated the breeding and adult population sizes, as well as the minimum population size, in a high-density brown bear population on the Shi...
Published in: | Ecology and Evolution |
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Online Access: | http://hdl.handle.net/2115/86851 https://doi.org/10.1002/ece3.9246 |
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fthokunivhus:oai:eprints.lib.hokudai.ac.jp:2115/86851 2023-05-15T18:42:09+02:00 Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears Shimozuru, Michito Jimbo, Mina Adachi, Keisuke Kawamura, Kei Shirane, Yuri Umemura, Yoshihiro Ishinazaka, Tsuyoshi Nakanishi, Masanao Kiyonari, Mayu Yamanaka, Masami Amagai, Yukihiro Ijuin, Ayaho Sakiyama, Tomoki Kasai, Shinsuke Nose, Takane Shirayanagi, Masataka Tsuruga, Hifumi Mano, Tsutomu Tsubota, Toshio Fukasawa, Keita Uno, Hiroyuki http://hdl.handle.net/2115/86851 https://doi.org/10.1002/ece3.9246 eng eng John Wiley & Sons http://hdl.handle.net/2115/86851 Ecology and evolution, 12(9): e9246 http://dx.doi.org/10.1002/ece3.9246 Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears/Michito Shimozuru, Mina Jimbo, Keisuke Adachi, Kei Kawamura, Yuri Shirane, Yoshihiro Umemura, Tsuyoshi Ishinazaka, Masanao Nakanishi, Mayu Kiyonari, Masami Yamanaka, Yukihiro Amagai, Ayaho Ijuin, Tomoki Sakiyama, Shinsuke Kasai, Takane Nose, Masataka Shirayanagi, Hifumi Tsuruga, Tsutomu Mano, Toshio Tsubota, Keita Fukasawa, Hiroyuki Uno/Ecology and Evolution 12(9).Copyright (c) 2022 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/ CC-BY breeding population size brown bear microsatellite analysis pedigree reconstruction population estimate Ursus arctos 649 article fthokunivhus https://doi.org/10.1002/ece3.9246 2022-11-18T01:07:06Z Robust estimates of demographic parameters are critical for effective wildlife conservation and management but are difficult to obtain for elusive species. We estimated the breeding and adult population sizes, as well as the minimum population size, in a high-density brown bear population on the Shiretoko Peninsula, in Hokkaido, Japan, using DNA-based pedigree reconstruction. A total of 1288 individuals, collected in and around the Shiretoko Peninsula between 1998 and 2020, were genotyped at 21 microsatellite loci. Among them, 499 individuals were identified by intensive genetic sampling conducted in two consecutive years (2019 and 2020) mainly by noninvasive methods (e.g., hair and fecal DNA). Among them, both parents were assigned for 330 bears, and either maternity or paternity was assigned to 47 and 76 individuals, respectively. The subsequent pedigree reconstruction indicated a range of breeding and adult (>= 4 years old) population sizes: 128-173 for female breeders and 66-91 male breeders, and 155-200 for female adults and 84-109 male adults. The minimum population size was estimated to be 449 (252 females and 197 males) in 2019. Long-term continuous genetic sampling prior to a short-term intensive survey would enable parentage to be identified in a population with a high probability, thus enabling reliable estimates of breeding population size for elusive species. Article in Journal/Newspaper Ursus arctos Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) Ecology and Evolution 12 9 |
institution |
Open Polar |
collection |
Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) |
op_collection_id |
fthokunivhus |
language |
English |
topic |
breeding population size brown bear microsatellite analysis pedigree reconstruction population estimate Ursus arctos 649 |
spellingShingle |
breeding population size brown bear microsatellite analysis pedigree reconstruction population estimate Ursus arctos 649 Shimozuru, Michito Jimbo, Mina Adachi, Keisuke Kawamura, Kei Shirane, Yuri Umemura, Yoshihiro Ishinazaka, Tsuyoshi Nakanishi, Masanao Kiyonari, Mayu Yamanaka, Masami Amagai, Yukihiro Ijuin, Ayaho Sakiyama, Tomoki Kasai, Shinsuke Nose, Takane Shirayanagi, Masataka Tsuruga, Hifumi Mano, Tsutomu Tsubota, Toshio Fukasawa, Keita Uno, Hiroyuki Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears |
topic_facet |
breeding population size brown bear microsatellite analysis pedigree reconstruction population estimate Ursus arctos 649 |
description |
Robust estimates of demographic parameters are critical for effective wildlife conservation and management but are difficult to obtain for elusive species. We estimated the breeding and adult population sizes, as well as the minimum population size, in a high-density brown bear population on the Shiretoko Peninsula, in Hokkaido, Japan, using DNA-based pedigree reconstruction. A total of 1288 individuals, collected in and around the Shiretoko Peninsula between 1998 and 2020, were genotyped at 21 microsatellite loci. Among them, 499 individuals were identified by intensive genetic sampling conducted in two consecutive years (2019 and 2020) mainly by noninvasive methods (e.g., hair and fecal DNA). Among them, both parents were assigned for 330 bears, and either maternity or paternity was assigned to 47 and 76 individuals, respectively. The subsequent pedigree reconstruction indicated a range of breeding and adult (>= 4 years old) population sizes: 128-173 for female breeders and 66-91 male breeders, and 155-200 for female adults and 84-109 male adults. The minimum population size was estimated to be 449 (252 females and 197 males) in 2019. Long-term continuous genetic sampling prior to a short-term intensive survey would enable parentage to be identified in a population with a high probability, thus enabling reliable estimates of breeding population size for elusive species. |
format |
Article in Journal/Newspaper |
author |
Shimozuru, Michito Jimbo, Mina Adachi, Keisuke Kawamura, Kei Shirane, Yuri Umemura, Yoshihiro Ishinazaka, Tsuyoshi Nakanishi, Masanao Kiyonari, Mayu Yamanaka, Masami Amagai, Yukihiro Ijuin, Ayaho Sakiyama, Tomoki Kasai, Shinsuke Nose, Takane Shirayanagi, Masataka Tsuruga, Hifumi Mano, Tsutomu Tsubota, Toshio Fukasawa, Keita Uno, Hiroyuki |
author_facet |
Shimozuru, Michito Jimbo, Mina Adachi, Keisuke Kawamura, Kei Shirane, Yuri Umemura, Yoshihiro Ishinazaka, Tsuyoshi Nakanishi, Masanao Kiyonari, Mayu Yamanaka, Masami Amagai, Yukihiro Ijuin, Ayaho Sakiyama, Tomoki Kasai, Shinsuke Nose, Takane Shirayanagi, Masataka Tsuruga, Hifumi Mano, Tsutomu Tsubota, Toshio Fukasawa, Keita Uno, Hiroyuki |
author_sort |
Shimozuru, Michito |
title |
Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears |
title_short |
Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears |
title_full |
Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears |
title_fullStr |
Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears |
title_full_unstemmed |
Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears |
title_sort |
estimation of breeding population size using dna-based pedigree reconstruction in brown bears |
publisher |
John Wiley & Sons |
url |
http://hdl.handle.net/2115/86851 https://doi.org/10.1002/ece3.9246 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
http://hdl.handle.net/2115/86851 Ecology and evolution, 12(9): e9246 http://dx.doi.org/10.1002/ece3.9246 |
op_rights |
Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears/Michito Shimozuru, Mina Jimbo, Keisuke Adachi, Kei Kawamura, Yuri Shirane, Yoshihiro Umemura, Tsuyoshi Ishinazaka, Masanao Nakanishi, Mayu Kiyonari, Masami Yamanaka, Yukihiro Amagai, Ayaho Ijuin, Tomoki Sakiyama, Shinsuke Kasai, Takane Nose, Masataka Shirayanagi, Hifumi Tsuruga, Tsutomu Mano, Toshio Tsubota, Keita Fukasawa, Hiroyuki Uno/Ecology and Evolution 12(9).Copyright (c) 2022 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.9246 |
container_title |
Ecology and Evolution |
container_volume |
12 |
container_issue |
9 |
_version_ |
1766231761656741888 |