Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka
We reported the genetic evidence of circulating hantaviruses from small mammals captured in a chronic kidney disease of unknown etiology (CKDu) hotspot area of Sri Lanka. The high seroprevalence of anti-hantavirus antibodies against Thailand orthohantavirus (THAIV) has been reported among CKDu patie...
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Online Access: | http://hdl.handle.net/2115/83963 https://doi.org/10.3390/v13101984 |
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fthokunivhus:oai:eprints.lib.hokudai.ac.jp:2115/83963 2023-05-15T18:05:18+02:00 Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka Muthusinghe, Devinda S. Shimizu, Kenta Lokupathirage, Sithumini M. W. Wei, Zhouoxing Sarathkumara, Yomani D. Fonseka, G. R. Amanda Senarathne, Pavani Koizumi, Nobuo Kawakami, Tomonori Koizumi, Akio Wickramasinghe, Chaminda Ebihara, Hideki Matsuno, Keita Tsuda, Yoshimi Arikawa, Jiro Gamage, Chandika D. Yoshimatsu, Kumiko http://hdl.handle.net/2115/83963 https://doi.org/10.3390/v13101984 eng eng MDPI http://hdl.handle.net/2115/83963 Viruses-Basel, 13(10): 1984 http://dx.doi.org/10.3390/v13101984 hantavirus Mus booduga Thailand orthohantavirus Anjozorobe hantavirus 490 article fthokunivhus https://doi.org/10.3390/v13101984 2022-11-18T01:06:46Z We reported the genetic evidence of circulating hantaviruses from small mammals captured in a chronic kidney disease of unknown etiology (CKDu) hotspot area of Sri Lanka. The high seroprevalence of anti-hantavirus antibodies against Thailand orthohantavirus (THAIV) has been reported among CKDu patients and rodents in Sri Lankan CKDu hotspots. We captured 116 small mammals from CKDu endemic regions in the Polonnaruwa District of Sri Lanka. Seven animals (five out of 11 Mus booduga and two out of 99 Rattus rattus) were PCR-positive for the hantavirus. A rat-borne sequence was grouped with a THAIV-like Anjozorobe virus. In contrast, Mus-borne sequences belonged to the THAIV lineage, suggesting a novel orthohantavirus species according to the phylogenetic analyses and whole-genome comparisons. Our genetic evidence indicates the presence of two THAIV-related viruses circulating in this CKDu endemic area, suggesting a basis for further investigations to identify the infectious virus in patients with CKDu and the CKDu induction mechanism of these viruses. Article in Journal/Newspaper Rattus rattus Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) Viruses 13 10 1984 |
institution |
Open Polar |
collection |
Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) |
op_collection_id |
fthokunivhus |
language |
English |
topic |
hantavirus Mus booduga Thailand orthohantavirus Anjozorobe hantavirus 490 |
spellingShingle |
hantavirus Mus booduga Thailand orthohantavirus Anjozorobe hantavirus 490 Muthusinghe, Devinda S. Shimizu, Kenta Lokupathirage, Sithumini M. W. Wei, Zhouoxing Sarathkumara, Yomani D. Fonseka, G. R. Amanda Senarathne, Pavani Koizumi, Nobuo Kawakami, Tomonori Koizumi, Akio Wickramasinghe, Chaminda Ebihara, Hideki Matsuno, Keita Tsuda, Yoshimi Arikawa, Jiro Gamage, Chandika D. Yoshimatsu, Kumiko Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka |
topic_facet |
hantavirus Mus booduga Thailand orthohantavirus Anjozorobe hantavirus 490 |
description |
We reported the genetic evidence of circulating hantaviruses from small mammals captured in a chronic kidney disease of unknown etiology (CKDu) hotspot area of Sri Lanka. The high seroprevalence of anti-hantavirus antibodies against Thailand orthohantavirus (THAIV) has been reported among CKDu patients and rodents in Sri Lankan CKDu hotspots. We captured 116 small mammals from CKDu endemic regions in the Polonnaruwa District of Sri Lanka. Seven animals (five out of 11 Mus booduga and two out of 99 Rattus rattus) were PCR-positive for the hantavirus. A rat-borne sequence was grouped with a THAIV-like Anjozorobe virus. In contrast, Mus-borne sequences belonged to the THAIV lineage, suggesting a novel orthohantavirus species according to the phylogenetic analyses and whole-genome comparisons. Our genetic evidence indicates the presence of two THAIV-related viruses circulating in this CKDu endemic area, suggesting a basis for further investigations to identify the infectious virus in patients with CKDu and the CKDu induction mechanism of these viruses. |
format |
Article in Journal/Newspaper |
author |
Muthusinghe, Devinda S. Shimizu, Kenta Lokupathirage, Sithumini M. W. Wei, Zhouoxing Sarathkumara, Yomani D. Fonseka, G. R. Amanda Senarathne, Pavani Koizumi, Nobuo Kawakami, Tomonori Koizumi, Akio Wickramasinghe, Chaminda Ebihara, Hideki Matsuno, Keita Tsuda, Yoshimi Arikawa, Jiro Gamage, Chandika D. Yoshimatsu, Kumiko |
author_facet |
Muthusinghe, Devinda S. Shimizu, Kenta Lokupathirage, Sithumini M. W. Wei, Zhouoxing Sarathkumara, Yomani D. Fonseka, G. R. Amanda Senarathne, Pavani Koizumi, Nobuo Kawakami, Tomonori Koizumi, Akio Wickramasinghe, Chaminda Ebihara, Hideki Matsuno, Keita Tsuda, Yoshimi Arikawa, Jiro Gamage, Chandika D. Yoshimatsu, Kumiko |
author_sort |
Muthusinghe, Devinda S. |
title |
Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka |
title_short |
Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka |
title_full |
Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka |
title_fullStr |
Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka |
title_full_unstemmed |
Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka |
title_sort |
identification of novel rodent-borne orthohantaviruses in an endemic area of chronic kidney disease of unknown etiology (ckdu) in sri lanka |
publisher |
MDPI |
url |
http://hdl.handle.net/2115/83963 https://doi.org/10.3390/v13101984 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
http://hdl.handle.net/2115/83963 Viruses-Basel, 13(10): 1984 http://dx.doi.org/10.3390/v13101984 |
op_doi |
https://doi.org/10.3390/v13101984 |
container_title |
Viruses |
container_volume |
13 |
container_issue |
10 |
container_start_page |
1984 |
_version_ |
1766176778419699712 |