Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species
Birds are exposed to many xenobiotics during their lifetime. For accurate prediction of xenobiotic-induced toxic effects on avian species, it is necessary to understand metabolic capacities in a comprehensive range of bird species. However, there is a lack of information about avian xenobiotic metab...
Published in: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
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Online Access: | http://hdl.handle.net/2115/76849 https://doi.org/10.1016/j.cbpc.2018.11.001 |
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fthokunivhus:oai:eprints.lib.hokudai.ac.jp:2115/76849 2023-05-15T18:44:07+02:00 Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species Kawai, Yusuke K. Shinya, So Ikenaka, Yoshinori Saengtienchai, Aksorn Kondo, Takamitsu Darvvish, Wageh Sobhy Nakayama, Shota M. M. Mizukawa, Hazuki Ishizuka, Mayumi http://hdl.handle.net/2115/76849 https://doi.org/10.1016/j.cbpc.2018.11.001 eng eng Elsevier http://hdl.handle.net/2115/76849 Comparative biochemistry and physiology Part C: Toxicology & pharmacology, 217: 5-14 http://dx.doi.org/10.1016/j.cbpc.2018.11.001 https://creativecommons.org/licenses/by-nc-nd/4.0/ CC-BY-NC-ND UGTs Xenobiotic Bird Wildlife Toxicology 649 article (author version) fthokunivhus https://doi.org/10.1016/j.cbpc.2018.11.001 2022-11-18T01:05:24Z Birds are exposed to many xenobiotics during their lifetime. For accurate prediction of xenobiotic-induced toxic effects on avian species, it is necessary to understand metabolic capacities in a comprehensive range of bird species. However, there is a lack of information about avian xenobiotic metabolizing enzymes (XMEs), particularly in wild birds. Uridine diphosphate glucuronosyltransferase (UGT) is an XME that plays an important role in phase II metabolism in the livers of mammals and birds. This study was performed to determine the characteristics of UGT1E isoform in avian species, those are related to mammals UGT 1A. To understand the characteristics of avian UGT1E isoforms, in vitro metabolic activity and genetic characteristics were investigated. Furthermore, mRNA expression levels of all chicken UGT1E isoforms were measured. On in vitro enzymatic analysis, the white-tailed eagle, great horned owl, and Humboldt penguin showed lower UGT-dependent activity than domestic birds. In synteny analysis, carnivorous birds were shown to have fewer UGT1E isoforms than herbivorous and omnivorous birds, which may explain why they have lower in vitro UGT activity. These observations suggested that raptors and seabirds, in which UGT activity is low, may be at high risk if exposed to elevated levels of xenobiotics in the environment. Phylogenetic analysis suggested that avian UGT1Es have evolved independently from mammalian UGT1As. We identified the important UGT isoforms, such as UGT1E13, and suspected their substrate specificities in avian xenobiotic metabolism by phylogenetic and quantitative realtime PCR analysis. This is the first report regarding the genetic characteristics and interspecies differences of UGT1Es in avian species. Article in Journal/Newspaper White-tailed eagle Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 217 5 14 |
institution |
Open Polar |
collection |
Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) |
op_collection_id |
fthokunivhus |
language |
English |
topic |
UGTs Xenobiotic Bird Wildlife Toxicology 649 |
spellingShingle |
UGTs Xenobiotic Bird Wildlife Toxicology 649 Kawai, Yusuke K. Shinya, So Ikenaka, Yoshinori Saengtienchai, Aksorn Kondo, Takamitsu Darvvish, Wageh Sobhy Nakayama, Shota M. M. Mizukawa, Hazuki Ishizuka, Mayumi Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species |
topic_facet |
UGTs Xenobiotic Bird Wildlife Toxicology 649 |
description |
Birds are exposed to many xenobiotics during their lifetime. For accurate prediction of xenobiotic-induced toxic effects on avian species, it is necessary to understand metabolic capacities in a comprehensive range of bird species. However, there is a lack of information about avian xenobiotic metabolizing enzymes (XMEs), particularly in wild birds. Uridine diphosphate glucuronosyltransferase (UGT) is an XME that plays an important role in phase II metabolism in the livers of mammals and birds. This study was performed to determine the characteristics of UGT1E isoform in avian species, those are related to mammals UGT 1A. To understand the characteristics of avian UGT1E isoforms, in vitro metabolic activity and genetic characteristics were investigated. Furthermore, mRNA expression levels of all chicken UGT1E isoforms were measured. On in vitro enzymatic analysis, the white-tailed eagle, great horned owl, and Humboldt penguin showed lower UGT-dependent activity than domestic birds. In synteny analysis, carnivorous birds were shown to have fewer UGT1E isoforms than herbivorous and omnivorous birds, which may explain why they have lower in vitro UGT activity. These observations suggested that raptors and seabirds, in which UGT activity is low, may be at high risk if exposed to elevated levels of xenobiotics in the environment. Phylogenetic analysis suggested that avian UGT1Es have evolved independently from mammalian UGT1As. We identified the important UGT isoforms, such as UGT1E13, and suspected their substrate specificities in avian xenobiotic metabolism by phylogenetic and quantitative realtime PCR analysis. This is the first report regarding the genetic characteristics and interspecies differences of UGT1Es in avian species. |
format |
Article in Journal/Newspaper |
author |
Kawai, Yusuke K. Shinya, So Ikenaka, Yoshinori Saengtienchai, Aksorn Kondo, Takamitsu Darvvish, Wageh Sobhy Nakayama, Shota M. M. Mizukawa, Hazuki Ishizuka, Mayumi |
author_facet |
Kawai, Yusuke K. Shinya, So Ikenaka, Yoshinori Saengtienchai, Aksorn Kondo, Takamitsu Darvvish, Wageh Sobhy Nakayama, Shota M. M. Mizukawa, Hazuki Ishizuka, Mayumi |
author_sort |
Kawai, Yusuke K. |
title |
Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species |
title_short |
Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species |
title_full |
Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species |
title_fullStr |
Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species |
title_full_unstemmed |
Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species |
title_sort |
characterization of function and genetic feature of udp-glucuronosyltransferase in avian species |
publisher |
Elsevier |
url |
http://hdl.handle.net/2115/76849 https://doi.org/10.1016/j.cbpc.2018.11.001 |
genre |
White-tailed eagle |
genre_facet |
White-tailed eagle |
op_relation |
http://hdl.handle.net/2115/76849 Comparative biochemistry and physiology Part C: Toxicology & pharmacology, 217: 5-14 http://dx.doi.org/10.1016/j.cbpc.2018.11.001 |
op_rights |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.cbpc.2018.11.001 |
container_title |
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
container_volume |
217 |
container_start_page |
5 |
op_container_end_page |
14 |
_version_ |
1766234673868963840 |