The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan)
Melting of sea-ice samples is an inevitable step in obtaining reliable and representative measurements for biogeochemical parameters such as inorganic nutrients and particulate matter. The impact of the sea-ice melting procedure has been previously evaluated for biological parameters such as chlorop...
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fthokunivhus:oai:eprints.lib.hokudai.ac.jp:2115/76673 2023-05-15T18:01:58+02:00 The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan) Roukaerts, Arnout Nomura, Daiki Deman, Florian Hattori, Hiroshi Dehairs, Frank Fripiat, François http://hdl.handle.net/2115/76673 https://doi.org/10.1007/s00300-018-2426-y eng eng http://hdl.handle.net/2115/76673 Polar Biology, 42(2): 347-356 http://dx.doi.org/10.1007/s00300-018-2426-y This is a post-peer-review, pre-copyedit version of an article published in Polar Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00300-018-2426-y Sea ice Direct melting Buffered melting Nutrients Particulate matter Chlorophyll a Sea of Okhotsk 452 article (author version) fthokunivhus https://doi.org/10.1007/s00300-018-2426-y 2022-11-18T01:05:10Z Melting of sea-ice samples is an inevitable step in obtaining reliable and representative measurements for biogeochemical parameters such as inorganic nutrients and particulate matter. The impact of the sea-ice melting procedure has been previously evaluated for biological parameters such as chlorophyll a and cell abundance. For nutrient and biomass concentrations in sea ice, it is generally considered to be best practice to melt samples fast; however, no systematic evaluation exists in literature. The impact of melting temperature and buffer addition to avoid osmotic shock was tested on ice samples in Saroma-ko Lagoon on the northeastern coast of Hokkaido, Japan. The focus was on inorganic nutrient concentrations (NO3−, NO2−, PO4−, NH4+, Si(OH)4) and particulate organic carbon and nitrogen concentrations and their isotope ratios. Coherent small changes have been observed for the parameter related to nitrogen, suggesting marginal cell lysis of a specific part of the microbial community. When differences are statistically significant, they are close to the uncertainty of the measurements and small in regard to the expected natural variation in sea ice. Our study suggest a minimal effect between melting treatments on biomass (POC, PN, and Chl a) and nutrient measurements in diatom-dominated sea ice and should be repeated where the sympagic community is dominated by flagellates. Article in Journal/Newspaper Polar Biology Sea ice Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) Okhotsk Polar Biology 42 2 347 356 |
institution |
Open Polar |
collection |
Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) |
op_collection_id |
fthokunivhus |
language |
English |
topic |
Sea ice Direct melting Buffered melting Nutrients Particulate matter Chlorophyll a Sea of Okhotsk 452 |
spellingShingle |
Sea ice Direct melting Buffered melting Nutrients Particulate matter Chlorophyll a Sea of Okhotsk 452 Roukaerts, Arnout Nomura, Daiki Deman, Florian Hattori, Hiroshi Dehairs, Frank Fripiat, François The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan) |
topic_facet |
Sea ice Direct melting Buffered melting Nutrients Particulate matter Chlorophyll a Sea of Okhotsk 452 |
description |
Melting of sea-ice samples is an inevitable step in obtaining reliable and representative measurements for biogeochemical parameters such as inorganic nutrients and particulate matter. The impact of the sea-ice melting procedure has been previously evaluated for biological parameters such as chlorophyll a and cell abundance. For nutrient and biomass concentrations in sea ice, it is generally considered to be best practice to melt samples fast; however, no systematic evaluation exists in literature. The impact of melting temperature and buffer addition to avoid osmotic shock was tested on ice samples in Saroma-ko Lagoon on the northeastern coast of Hokkaido, Japan. The focus was on inorganic nutrient concentrations (NO3−, NO2−, PO4−, NH4+, Si(OH)4) and particulate organic carbon and nitrogen concentrations and their isotope ratios. Coherent small changes have been observed for the parameter related to nitrogen, suggesting marginal cell lysis of a specific part of the microbial community. When differences are statistically significant, they are close to the uncertainty of the measurements and small in regard to the expected natural variation in sea ice. Our study suggest a minimal effect between melting treatments on biomass (POC, PN, and Chl a) and nutrient measurements in diatom-dominated sea ice and should be repeated where the sympagic community is dominated by flagellates. |
format |
Article in Journal/Newspaper |
author |
Roukaerts, Arnout Nomura, Daiki Deman, Florian Hattori, Hiroshi Dehairs, Frank Fripiat, François |
author_facet |
Roukaerts, Arnout Nomura, Daiki Deman, Florian Hattori, Hiroshi Dehairs, Frank Fripiat, François |
author_sort |
Roukaerts, Arnout |
title |
The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan) |
title_short |
The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan) |
title_full |
The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan) |
title_fullStr |
The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan) |
title_full_unstemmed |
The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan) |
title_sort |
effect of melting treatments on the assessment of biomass and nutrients in sea ice (saroma-ko lagoon, hokkaido, japan) |
url |
http://hdl.handle.net/2115/76673 https://doi.org/10.1007/s00300-018-2426-y |
geographic |
Okhotsk |
geographic_facet |
Okhotsk |
genre |
Polar Biology Sea ice |
genre_facet |
Polar Biology Sea ice |
op_relation |
http://hdl.handle.net/2115/76673 Polar Biology, 42(2): 347-356 http://dx.doi.org/10.1007/s00300-018-2426-y |
op_rights |
This is a post-peer-review, pre-copyedit version of an article published in Polar Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00300-018-2426-y |
op_doi |
https://doi.org/10.1007/s00300-018-2426-y |
container_title |
Polar Biology |
container_volume |
42 |
container_issue |
2 |
container_start_page |
347 |
op_container_end_page |
356 |
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1766171580760588288 |