Antigenic and genetic analyses of avian influenza viruses isolated in Japan
H5 and H9 influenza virus isolates in Japan were genetically and antigenically characterized and the birds infected with the virus were pathologically examined. In 2005, H5N2 influenza viruses were isolated form layer chickens in Ibaraki Prefecture, Japan and the Ibaraki prefectural governor declare...
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fthokunivhus:oai:eprints.lib.hokudai.ac.jp:2115/44513 2023-05-15T15:59:50+02:00 Antigenic and genetic analyses of avian influenza viruses isolated in Japan 日本で分離された鳥インフルエンザウイルスの 抗原性と遺伝子の解析 Okamatsu, Masatoshi 63p. http://hdl.handle.net/2115/44513 https://doi.org/10.14943/doctoral.r6753 eng eng Hokkaido University http://hdl.handle.net/2115/44513 doi:10.14943/doctoral.r6753 北海道大学. 博士(獣医学) 649 theses (doctoral) fthokunivhus https://doi.org/10.14943/doctoral.r6753 2022-11-18T01:02:04Z H5 and H9 influenza virus isolates in Japan were genetically and antigenically characterized and the birds infected with the virus were pathologically examined. In 2005, H5N2 influenza viruses were isolated form layer chickens in Ibaraki Prefecture, Japan and the Ibaraki prefectural governor declared the end of the H5N2 outbreak in June, 2006, 1 year after the first detection. Sixteen H5N2 viruses were isolated from 9 farms of the affected 41 farms. Phylogenetic and antigenic analysis of the isolates showed that these isolates were closely related to the H5N2 strains prevalent in Central America that have been circulating since 1994. Experimental infection of chickens with the index isolate (A/chicken/Ibaraki/1/2005 (H5N2)) demonstrated that this virus replicated efficiently in the respiratory tract without clinical signs. Pathological findings of the chickens indicated that the virus efficiently replicated in salivary epithelial cells. The virus was transmitted among the chickens in separated cage. This result indicated that the infection to chickens spread by droplet transmission in the outbreaks. In 2008, H5N1 highly pathogenic avian influenza virus was isolated from whooper swans (Cygnus cygnus) found dead in Hokkaido, Japan. Pathological findings indicate that the swan died due to severe congestive edema in the lungs. Phylogenetic analysis of the HA genes of the isolates revealed that these are the progeny viruses of the isolates Clade 2.3.2 from poultry and wild birds in China, Russia, Korea, and Hong Kong. Antigenic analyses indicated that the viruses are different from the H5N1 viruses isolated from wild birds and poultry before 2007. The chickens vaccinated with A/duck/Hokkaido/Vac-1/2004 (H5N1) survived 14 days after challenge with the isolate, although small amount of the challenge virus was recovered from the tissues of the birds. These findings indicate that the H5N1 highly pathogenic avian influenza virus are circulating in wild birds in addition to domestic poultry in Asia and showing antigenic ... Other/Unknown Material Cygnus cygnus Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) |
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649 Okamatsu, Masatoshi Antigenic and genetic analyses of avian influenza viruses isolated in Japan |
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H5 and H9 influenza virus isolates in Japan were genetically and antigenically characterized and the birds infected with the virus were pathologically examined. In 2005, H5N2 influenza viruses were isolated form layer chickens in Ibaraki Prefecture, Japan and the Ibaraki prefectural governor declared the end of the H5N2 outbreak in June, 2006, 1 year after the first detection. Sixteen H5N2 viruses were isolated from 9 farms of the affected 41 farms. Phylogenetic and antigenic analysis of the isolates showed that these isolates were closely related to the H5N2 strains prevalent in Central America that have been circulating since 1994. Experimental infection of chickens with the index isolate (A/chicken/Ibaraki/1/2005 (H5N2)) demonstrated that this virus replicated efficiently in the respiratory tract without clinical signs. Pathological findings of the chickens indicated that the virus efficiently replicated in salivary epithelial cells. The virus was transmitted among the chickens in separated cage. This result indicated that the infection to chickens spread by droplet transmission in the outbreaks. In 2008, H5N1 highly pathogenic avian influenza virus was isolated from whooper swans (Cygnus cygnus) found dead in Hokkaido, Japan. Pathological findings indicate that the swan died due to severe congestive edema in the lungs. Phylogenetic analysis of the HA genes of the isolates revealed that these are the progeny viruses of the isolates Clade 2.3.2 from poultry and wild birds in China, Russia, Korea, and Hong Kong. Antigenic analyses indicated that the viruses are different from the H5N1 viruses isolated from wild birds and poultry before 2007. The chickens vaccinated with A/duck/Hokkaido/Vac-1/2004 (H5N1) survived 14 days after challenge with the isolate, although small amount of the challenge virus was recovered from the tissues of the birds. These findings indicate that the H5N1 highly pathogenic avian influenza virus are circulating in wild birds in addition to domestic poultry in Asia and showing antigenic ... |
format |
Other/Unknown Material |
author |
Okamatsu, Masatoshi |
author_facet |
Okamatsu, Masatoshi |
author_sort |
Okamatsu, Masatoshi |
title |
Antigenic and genetic analyses of avian influenza viruses isolated in Japan |
title_short |
Antigenic and genetic analyses of avian influenza viruses isolated in Japan |
title_full |
Antigenic and genetic analyses of avian influenza viruses isolated in Japan |
title_fullStr |
Antigenic and genetic analyses of avian influenza viruses isolated in Japan |
title_full_unstemmed |
Antigenic and genetic analyses of avian influenza viruses isolated in Japan |
title_sort |
antigenic and genetic analyses of avian influenza viruses isolated in japan |
publisher |
Hokkaido University |
url |
http://hdl.handle.net/2115/44513 https://doi.org/10.14943/doctoral.r6753 |
genre |
Cygnus cygnus |
genre_facet |
Cygnus cygnus |
op_relation |
http://hdl.handle.net/2115/44513 doi:10.14943/doctoral.r6753 北海道大学. 博士(獣医学) |
op_doi |
https://doi.org/10.14943/doctoral.r6753 |
_version_ |
1766395735664754688 |