Temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic Pacific (SEEDS-II)
Little is known about the effects of iron enrichment in high-nutrient, low chlorophyll (HNLC) waters on the community composition of heterotrophic bacteria, which are crucial to nutrient recycling and microbial food webs. Using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments, we...
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fthokunivhus:oai:eprints.lib.hokudai.ac.jp:2115/44029 2023-05-15T18:28:15+02:00 Temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic Pacific (SEEDS-II) Kataoka, Takafumi Suzuki, Koji Hayakawa, Maki Kudo, Isao Higashi, Seigo Tsuda, Atsushi http://hdl.handle.net/2115/44029 https://doi.org/10.1016/j.dsr2.2009.06.013 eng eng Elsevier http://hdl.handle.net/2115/44029 Deep Sea Research Part II : Topical Studies in Oceanography, 56(26): 2779-2787 http://dx.doi.org/10.1016/j.dsr2.2009.06.013 16S rRNA gene Bacterial community Eubacteria Iron enrichment PCR-DGGE SEEDS-II 452 article (author version) fthokunivhus https://doi.org/10.1016/j.dsr2.2009.06.013 2022-11-18T01:02:04Z Little is known about the effects of iron enrichment in high-nutrient, low chlorophyll (HNLC) waters on the community composition of heterotrophic bacteria, which are crucial to nutrient recycling and microbial food webs. Using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments, we investigated the heterotrophic eubacterial community composition in surface waters during an in situ iron enrichment experiment (SEEDS-II) in the western subarctic Pacific during the summer of 2004. DGGE fingerprints representing the community composition of eubacteria differed inside and outside the iron-enriched patch. Sequencing of DGGE bands revealed that at least five phylotypes of α-proteobacteria including Roseobacter, Cytophaga-Flavobacteria-Bacteroides (CFB), γ-proteobacteria, and Actinobacteria occurred in almost all samples from the iron-enriched patch. Diatoms did not bloom during SEEDS-II, but the eubacterial composition in the iron-enriched patch was similar to that in diatom blooms observed previously. Although dissolved organic carbon DOC accumulation was not detected in surface waters during SEEDS-II, growth of the Roseobacter clade might have been particularly stimulated after iron additions. Two identified phylotypes of CFB were closely related to the genus Saprospira, whose algicidal activity might degrade the phytoplankton assemblages increased by iron enrichment. These results suggest that the responses of heterotrophic bacteria to iron enrichment could differ among phylotypes during SEEDS-II. Article in Journal/Newspaper Subarctic Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) Pacific Deep Sea Research Part II: Topical Studies in Oceanography 56 26 2779 2787 |
institution |
Open Polar |
collection |
Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) |
op_collection_id |
fthokunivhus |
language |
English |
topic |
16S rRNA gene Bacterial community Eubacteria Iron enrichment PCR-DGGE SEEDS-II 452 |
spellingShingle |
16S rRNA gene Bacterial community Eubacteria Iron enrichment PCR-DGGE SEEDS-II 452 Kataoka, Takafumi Suzuki, Koji Hayakawa, Maki Kudo, Isao Higashi, Seigo Tsuda, Atsushi Temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic Pacific (SEEDS-II) |
topic_facet |
16S rRNA gene Bacterial community Eubacteria Iron enrichment PCR-DGGE SEEDS-II 452 |
description |
Little is known about the effects of iron enrichment in high-nutrient, low chlorophyll (HNLC) waters on the community composition of heterotrophic bacteria, which are crucial to nutrient recycling and microbial food webs. Using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments, we investigated the heterotrophic eubacterial community composition in surface waters during an in situ iron enrichment experiment (SEEDS-II) in the western subarctic Pacific during the summer of 2004. DGGE fingerprints representing the community composition of eubacteria differed inside and outside the iron-enriched patch. Sequencing of DGGE bands revealed that at least five phylotypes of α-proteobacteria including Roseobacter, Cytophaga-Flavobacteria-Bacteroides (CFB), γ-proteobacteria, and Actinobacteria occurred in almost all samples from the iron-enriched patch. Diatoms did not bloom during SEEDS-II, but the eubacterial composition in the iron-enriched patch was similar to that in diatom blooms observed previously. Although dissolved organic carbon DOC accumulation was not detected in surface waters during SEEDS-II, growth of the Roseobacter clade might have been particularly stimulated after iron additions. Two identified phylotypes of CFB were closely related to the genus Saprospira, whose algicidal activity might degrade the phytoplankton assemblages increased by iron enrichment. These results suggest that the responses of heterotrophic bacteria to iron enrichment could differ among phylotypes during SEEDS-II. |
format |
Article in Journal/Newspaper |
author |
Kataoka, Takafumi Suzuki, Koji Hayakawa, Maki Kudo, Isao Higashi, Seigo Tsuda, Atsushi |
author_facet |
Kataoka, Takafumi Suzuki, Koji Hayakawa, Maki Kudo, Isao Higashi, Seigo Tsuda, Atsushi |
author_sort |
Kataoka, Takafumi |
title |
Temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic Pacific (SEEDS-II) |
title_short |
Temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic Pacific (SEEDS-II) |
title_full |
Temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic Pacific (SEEDS-II) |
title_fullStr |
Temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic Pacific (SEEDS-II) |
title_full_unstemmed |
Temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic Pacific (SEEDS-II) |
title_sort |
temporal changes in community composition of heterotrophic bacteria during in situ iron enrichment in the western subarctic pacific (seeds-ii) |
publisher |
Elsevier |
url |
http://hdl.handle.net/2115/44029 https://doi.org/10.1016/j.dsr2.2009.06.013 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
http://hdl.handle.net/2115/44029 Deep Sea Research Part II : Topical Studies in Oceanography, 56(26): 2779-2787 http://dx.doi.org/10.1016/j.dsr2.2009.06.013 |
op_doi |
https://doi.org/10.1016/j.dsr2.2009.06.013 |
container_title |
Deep Sea Research Part II: Topical Studies in Oceanography |
container_volume |
56 |
container_issue |
26 |
container_start_page |
2779 |
op_container_end_page |
2787 |
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1766210644151894016 |