Purification and characterization of a novel incomplete-type vitellogenin protein (VgC) in Sakhalin taimen (Hucho perryi)

• Published in: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
• Main Authors: Amano, Haruna, Mochizuki, Machiko, Fujita, Toshiaki, Hiramatsu, Naoshi, Todo, Takashi, Hara, Akihiko
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier
• Subjects: Multiple vitellogenin; Purification; Quantification; Sakhalin taimen; 663; Sakhalin
• Online Access: http://hdl.handle.net/2115/43820; https://doi.org/10.1016/j.cbpa.2010.05.006

A novel, incomplete-type vitellogenin (VgC) and its derived yolk lipovitellin (LvC) were immunologically detected in female serum and egg extracts, respectively, of Sakhalin taimen (Hucho perryi) using a subtype-specific antiserum against LvC of grey mullet (Mugil cephalus). The taimen VgC was purified from the sera of vitellogenic females by a combination of gel filtration, anion exchange, and immunoadsorbent column chromatography. Gel filtration of the purified VgC revealed that it had an apparent native mass of ∼380 kDa, while the mass of the VgC polypeptide that appeared following SDS-PAGE was estimated to be ∼140 kDa. An antiserum was raised against the purified VgC and utilized for the development of a subtype-specific immunoassay for VgC. Levels of VgC in the serum of female taimen increased from 25 μg/mL to ∼1 mg/mL, with an increase of GSI. Levels of complete-type Vg and estradiol-17β (E2) in the serum of E2-administered juvenile taimen increased and reached peak levels similar to those found in vitellogenic females. Although VgC could be induced in the serum of E2-administered taimen, it stayed at levels (35.5-73 μg/mL) lower than those obtained in females. This is the first report on the presence of serum VgC and yolk LvC in a salmonid species; these findings indicate that for Sakhalin taimen, like other highly-evolved teleost species, this minor subtype of Vg is significant in the formation of egg yolk.