Vibrio splendidus biovar II as the causative agent of bacillary necrosis of Japanese oyster Crassostrea gigas.
Recurrent outbreaks of a disease leading to mass mortalities in an oyster (Crassostrea gigas) hatchery located in western Japan were investigated. The disease occurred regularly in 2- to 8-d-old larvae and has been experimentally controlled in the hatchery by treating the larval rearing water with s...
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2014
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fthiroshimauniv:oai:ir.lib.hiroshima-u.ac.jp:00025656 2023-05-15T15:57:44+02:00 Vibrio splendidus biovar II as the causative agent of bacillary necrosis of Japanese oyster Crassostrea gigas. Sugumar, G. Nakai, Toshihiro Hirata, Yasushi Matsubara, D. Muroga, Kiyokuni 2014-10-17 application/pdf http://ir.lib.hiroshima-u.ac.jp/00025656 http://ir.lib.hiroshima-u.ac.jp/files/public/2/25656/20141016152220990415/dao033p111.pdf eng eng Inter-Research http://dx.doi.org/10.3354/dao033111 doi:10.3354/dao033111 http://ir.lib.hiroshima-u.ac.jp/files/public/2/25656/20141016152220990415/dao033p111.pdf 0177-5103 1616-1580 AA10443976 http://ir.lib.hiroshima-u.ac.jp/00025656 Copyright (c) 1998 Inter-Research. Bacillary necrosis Vibriosis Crassostrea gigas Larvae Vibrio splendidus II Pathogenicity 480 Journal Article text 2014 fthiroshimauniv https://doi.org/10.3354/dao033111 2020-12-23T22:54:55Z Recurrent outbreaks of a disease leading to mass mortalities in an oyster (Crassostrea gigas) hatchery located in western Japan were investigated. The disease occurred regularly in 2- to 8-d-old larvae and has been experimentally controlled in the hatchery by treating the larval rearing water with streptomycin, without ascertaining the etiological agent. The signs of the disease and the course of infection resembled bacillary necrosis reported in oysters and other bivalve molluscs in the USA and Europe. Quantitative and qualitative examinations of the bacterial flora of hatchery samples including source water, broodstock, larval feed and larvae revealed a very high total bacterial load and presumptive vibrios in diseased larvae. Further, the bacterial profile revealed that Vibrio spp. constituted approximately 60 to 95% of the bacteria isolated from infected larvae and most isolates were identified as V. splendidus biovar II and V. harveyi, suggesting their possible role in the disease. However, experimental challenges proved the pathogenicity of V. splendidus II. Several isolates of V. splendidus II from infected larvae were highly pathogenic, producing 100% mortality at levels of 105 cfu ml-1 in 24 h, while isolates from other sources demonstrated a low degree of virulence. Detection of V. splendidus II from broodstock, especially in the gonad of a few breeders, suggests the probability that broodstock could be the source and route of transmission of this pathogen. Article in Journal/Newspaper Crassostrea gigas Hiroshima University: Institutional Repository (HiR) Diseases of Aquatic Organisms 33 111 118 |
institution |
Open Polar |
collection |
Hiroshima University: Institutional Repository (HiR) |
op_collection_id |
fthiroshimauniv |
language |
English |
topic |
Bacillary necrosis Vibriosis Crassostrea gigas Larvae Vibrio splendidus II Pathogenicity 480 |
spellingShingle |
Bacillary necrosis Vibriosis Crassostrea gigas Larvae Vibrio splendidus II Pathogenicity 480 Sugumar, G. Nakai, Toshihiro Hirata, Yasushi Matsubara, D. Muroga, Kiyokuni Vibrio splendidus biovar II as the causative agent of bacillary necrosis of Japanese oyster Crassostrea gigas. |
topic_facet |
Bacillary necrosis Vibriosis Crassostrea gigas Larvae Vibrio splendidus II Pathogenicity 480 |
description |
Recurrent outbreaks of a disease leading to mass mortalities in an oyster (Crassostrea gigas) hatchery located in western Japan were investigated. The disease occurred regularly in 2- to 8-d-old larvae and has been experimentally controlled in the hatchery by treating the larval rearing water with streptomycin, without ascertaining the etiological agent. The signs of the disease and the course of infection resembled bacillary necrosis reported in oysters and other bivalve molluscs in the USA and Europe. Quantitative and qualitative examinations of the bacterial flora of hatchery samples including source water, broodstock, larval feed and larvae revealed a very high total bacterial load and presumptive vibrios in diseased larvae. Further, the bacterial profile revealed that Vibrio spp. constituted approximately 60 to 95% of the bacteria isolated from infected larvae and most isolates were identified as V. splendidus biovar II and V. harveyi, suggesting their possible role in the disease. However, experimental challenges proved the pathogenicity of V. splendidus II. Several isolates of V. splendidus II from infected larvae were highly pathogenic, producing 100% mortality at levels of 105 cfu ml-1 in 24 h, while isolates from other sources demonstrated a low degree of virulence. Detection of V. splendidus II from broodstock, especially in the gonad of a few breeders, suggests the probability that broodstock could be the source and route of transmission of this pathogen. |
format |
Article in Journal/Newspaper |
author |
Sugumar, G. Nakai, Toshihiro Hirata, Yasushi Matsubara, D. Muroga, Kiyokuni |
author_facet |
Sugumar, G. Nakai, Toshihiro Hirata, Yasushi Matsubara, D. Muroga, Kiyokuni |
author_sort |
Sugumar, G. |
title |
Vibrio splendidus biovar II as the causative agent of bacillary necrosis of Japanese oyster Crassostrea gigas. |
title_short |
Vibrio splendidus biovar II as the causative agent of bacillary necrosis of Japanese oyster Crassostrea gigas. |
title_full |
Vibrio splendidus biovar II as the causative agent of bacillary necrosis of Japanese oyster Crassostrea gigas. |
title_fullStr |
Vibrio splendidus biovar II as the causative agent of bacillary necrosis of Japanese oyster Crassostrea gigas. |
title_full_unstemmed |
Vibrio splendidus biovar II as the causative agent of bacillary necrosis of Japanese oyster Crassostrea gigas. |
title_sort |
vibrio splendidus biovar ii as the causative agent of bacillary necrosis of japanese oyster crassostrea gigas. |
publisher |
Inter-Research |
publishDate |
2014 |
url |
http://ir.lib.hiroshima-u.ac.jp/00025656 http://ir.lib.hiroshima-u.ac.jp/files/public/2/25656/20141016152220990415/dao033p111.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://dx.doi.org/10.3354/dao033111 doi:10.3354/dao033111 http://ir.lib.hiroshima-u.ac.jp/files/public/2/25656/20141016152220990415/dao033p111.pdf 0177-5103 1616-1580 AA10443976 http://ir.lib.hiroshima-u.ac.jp/00025656 |
op_rights |
Copyright (c) 1998 Inter-Research. |
op_doi |
https://doi.org/10.3354/dao033111 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
33 |
container_start_page |
111 |
op_container_end_page |
118 |
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1766393430887366656 |