Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson’s Mouse Model
Parkinson’s disease (PD) is a commonly reported age-related neurodegenerative disorder. Microglial-mediated neuroinflammation is one of the cardinal hallmarks of various neurodegenerative disorders, including PD progression. Inadequate therapeutic strategies and substantial adverse effects of well-e...
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fthindawi:oai:hindawi.com:10.1155/2018/3175214 2023-05-15T16:01:21+02:00 Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson’s Mouse Model Shin-Young Park Govindarajan Karthivashan Hyun Myung Ko Duk-Yeon Cho Joonsoo Kim Dae Jun Cho Palanivel Ganesan In Su-Kim Dong-Kug Choi 2018 https://doi.org/10.1155/2018/3175214 en eng Oxidative Medicine and Cellular Longevity https://doi.org/10.1155/2018/3175214 Copyright © 2018 Shin-Young Park et al. Research Article 2018 fthindawi https://doi.org/10.1155/2018/3175214 2019-05-26T09:54:38Z Parkinson’s disease (PD) is a commonly reported age-related neurodegenerative disorder. Microglial-mediated neuroinflammation is one of the cardinal hallmarks of various neurodegenerative disorders, including PD progression. Inadequate therapeutic strategies and substantial adverse effects of well-established drug candidates demand new therapeutic leads to treat PD. Dendropanax morbifera (DM) is an endemic plant species of South Korea, and it has been used extensively as traditional medicine to treat numerous clinical complications. In this study, we conducted an initial profiling of the few major phytoconstituents of aqueous DM leaf extracts (DML) and quantified the same using high-performance liquid chromatography tandem mass spectrometry with electrospray ionization (HPLC-ESI-MS/MS). We subsequently evaluated the antineuroinflammatory activity and ameliorative potential of DML in both in vitro and in vivo experimental PD models. The prophylactic treatment of DML effectually improved the behavioral deficits, curbed the microglial-mediated neuroinflammation, and protected dopaminergic (DA) neuronal loss by restoring tyrosine hydroxylase (TH) levels in brain tissue of the MPTP-induced PD mouse model. We conducted chromatographic profiling and identified chlorogenic acid (CA) as a major constituent (19.5 mg/g of BuOH fraction), which has been well documented as an antioxidant and anti-inflammatory agent. This was found to be in harmony with our in vitro results, where DML suppressed the level of inflammatory mediators and allied the signaling pathway in LPS-stimulated microglial cells. The results of our study indicate that DML and its bioactive constituents can be developed as potential therapeutic candidates against progressive PD complications. Article in Journal/Newspaper DML Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity 2018 1 14 |
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Parkinson’s disease (PD) is a commonly reported age-related neurodegenerative disorder. Microglial-mediated neuroinflammation is one of the cardinal hallmarks of various neurodegenerative disorders, including PD progression. Inadequate therapeutic strategies and substantial adverse effects of well-established drug candidates demand new therapeutic leads to treat PD. Dendropanax morbifera (DM) is an endemic plant species of South Korea, and it has been used extensively as traditional medicine to treat numerous clinical complications. In this study, we conducted an initial profiling of the few major phytoconstituents of aqueous DM leaf extracts (DML) and quantified the same using high-performance liquid chromatography tandem mass spectrometry with electrospray ionization (HPLC-ESI-MS/MS). We subsequently evaluated the antineuroinflammatory activity and ameliorative potential of DML in both in vitro and in vivo experimental PD models. The prophylactic treatment of DML effectually improved the behavioral deficits, curbed the microglial-mediated neuroinflammation, and protected dopaminergic (DA) neuronal loss by restoring tyrosine hydroxylase (TH) levels in brain tissue of the MPTP-induced PD mouse model. We conducted chromatographic profiling and identified chlorogenic acid (CA) as a major constituent (19.5 mg/g of BuOH fraction), which has been well documented as an antioxidant and anti-inflammatory agent. This was found to be in harmony with our in vitro results, where DML suppressed the level of inflammatory mediators and allied the signaling pathway in LPS-stimulated microglial cells. The results of our study indicate that DML and its bioactive constituents can be developed as potential therapeutic candidates against progressive PD complications. |
format |
Article in Journal/Newspaper |
author |
Shin-Young Park Govindarajan Karthivashan Hyun Myung Ko Duk-Yeon Cho Joonsoo Kim Dae Jun Cho Palanivel Ganesan In Su-Kim Dong-Kug Choi |
spellingShingle |
Shin-Young Park Govindarajan Karthivashan Hyun Myung Ko Duk-Yeon Cho Joonsoo Kim Dae Jun Cho Palanivel Ganesan In Su-Kim Dong-Kug Choi Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson’s Mouse Model |
author_facet |
Shin-Young Park Govindarajan Karthivashan Hyun Myung Ko Duk-Yeon Cho Joonsoo Kim Dae Jun Cho Palanivel Ganesan In Su-Kim Dong-Kug Choi |
author_sort |
Shin-Young Park |
title |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson’s Mouse Model |
title_short |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson’s Mouse Model |
title_full |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson’s Mouse Model |
title_fullStr |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson’s Mouse Model |
title_full_unstemmed |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson’s Mouse Model |
title_sort |
aqueous extract of dendropanax morbiferus leaves effectively alleviated neuroinflammation and behavioral impediments in mptp-induced parkinson’s mouse model |
publisher |
Oxidative Medicine and Cellular Longevity |
publishDate |
2018 |
url |
https://doi.org/10.1155/2018/3175214 |
genre |
DML |
genre_facet |
DML |
op_relation |
https://doi.org/10.1155/2018/3175214 |
op_rights |
Copyright © 2018 Shin-Young Park et al. |
op_doi |
https://doi.org/10.1155/2018/3175214 |
container_title |
Oxidative Medicine and Cellular Longevity |
container_volume |
2018 |
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1 |
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14 |
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1766397247431376896 |