Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes
Advanced fluorescence analysis within the wide range of excitation wavelengths from 230 to 510 nm accompanied with chromatography was used to study natural chromophoric dissolved organic matter (CDOM) from three freshwater Karelian lakes. The influence of excitation wavelength (λex) on fluorescence...
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fthindawi:oai:hindawi.com:10.1155/2018/3168320 2023-05-15T17:01:35+02:00 Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes Svetlana Patsaeva Daria Khundzhua Oleg A. Trubetskoj Olga E. Trubetskaya 2018 https://doi.org/10.1155/2018/3168320 en eng Journal of Spectroscopy https://doi.org/10.1155/2018/3168320 Copyright © 2018 Svetlana Patsaeva et al. Research Article 2018 fthindawi https://doi.org/10.1155/2018/3168320 2019-05-26T10:11:36Z Advanced fluorescence analysis within the wide range of excitation wavelengths from 230 to 510 nm accompanied with chromatography was used to study natural chromophoric dissolved organic matter (CDOM) from three freshwater Karelian lakes. The influence of excitation wavelength (λex) on fluorescence quantum yield and emission maximum position was determined. The CDOM fluorescence quantum yield has reached a minimum at λex∼270–280 nm and a maximum at λex∼340–360 nm. It was monotonously decreasing after 370 nm towards longer excitation wavelengths. Analytical reversed-phase high-performance liquid chromatography with multiwavelength fluorescence detector characterized distribution of fluorophores between hydrophilic/hydrophobic CDOM parts. This technique revealed “hidden” protein-like fluorophores for some CDOM fractions, in spite of the absence of protein-like fluorescence in the initial CDOM samples. The humic-like fluorescence was documented for all hydrophilic and hydrophobic CDOM chromatographic peaks, and its intensity was decreasing along with peaks’ hydrophobicity. On contrary, the protein-like fluorescence was found only in the hydrophobic peaks, and its intensity was increasing along with peaks’ hydrophobicity. This work provides new data on the CDOM optical properties consistent with the formation of supramolecular assemblies controlled by association of low-molecular size components. In addition, these data are very useful for understanding the CDOM function in the environment. Article in Journal/Newspaper karelian Hindawi Publishing Corporation Journal of Spectroscopy 2018 1 7 |
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Open Polar |
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Hindawi Publishing Corporation |
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fthindawi |
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English |
description |
Advanced fluorescence analysis within the wide range of excitation wavelengths from 230 to 510 nm accompanied with chromatography was used to study natural chromophoric dissolved organic matter (CDOM) from three freshwater Karelian lakes. The influence of excitation wavelength (λex) on fluorescence quantum yield and emission maximum position was determined. The CDOM fluorescence quantum yield has reached a minimum at λex∼270–280 nm and a maximum at λex∼340–360 nm. It was monotonously decreasing after 370 nm towards longer excitation wavelengths. Analytical reversed-phase high-performance liquid chromatography with multiwavelength fluorescence detector characterized distribution of fluorophores between hydrophilic/hydrophobic CDOM parts. This technique revealed “hidden” protein-like fluorophores for some CDOM fractions, in spite of the absence of protein-like fluorescence in the initial CDOM samples. The humic-like fluorescence was documented for all hydrophilic and hydrophobic CDOM chromatographic peaks, and its intensity was decreasing along with peaks’ hydrophobicity. On contrary, the protein-like fluorescence was found only in the hydrophobic peaks, and its intensity was increasing along with peaks’ hydrophobicity. This work provides new data on the CDOM optical properties consistent with the formation of supramolecular assemblies controlled by association of low-molecular size components. In addition, these data are very useful for understanding the CDOM function in the environment. |
format |
Article in Journal/Newspaper |
author |
Svetlana Patsaeva Daria Khundzhua Oleg A. Trubetskoj Olga E. Trubetskaya |
spellingShingle |
Svetlana Patsaeva Daria Khundzhua Oleg A. Trubetskoj Olga E. Trubetskaya Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes |
author_facet |
Svetlana Patsaeva Daria Khundzhua Oleg A. Trubetskoj Olga E. Trubetskaya |
author_sort |
Svetlana Patsaeva |
title |
Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes |
title_short |
Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes |
title_full |
Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes |
title_fullStr |
Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes |
title_full_unstemmed |
Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes |
title_sort |
excitation-dependent fluorescence quantum yield for freshwater chromophoric dissolved organic matter from northern russian lakes |
publisher |
Journal of Spectroscopy |
publishDate |
2018 |
url |
https://doi.org/10.1155/2018/3168320 |
genre |
karelian |
genre_facet |
karelian |
op_relation |
https://doi.org/10.1155/2018/3168320 |
op_rights |
Copyright © 2018 Svetlana Patsaeva et al. |
op_doi |
https://doi.org/10.1155/2018/3168320 |
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Journal of Spectroscopy |
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2018 |
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1 |
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7 |
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1766054691946364928 |