Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium
A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo6+ to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybdate...
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fthindawi:oai:hindawi.com:10.1155/2013/871941 2023-05-15T13:53:26+02:00 Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium S. A. Ahmad M. Y. Shukor N. A. Shamaan W. P. Mac Cormack M. A. Syed 2013 https://doi.org/10.1155/2013/871941 en eng BioMed Research International https://doi.org/10.1155/2013/871941 Copyright © 2013 S. A. Ahmad et al. Microbiology Research Article 2013 fthindawi https://doi.org/10.1155/2013/871941 2019-05-26T03:53:06Z A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo6+ to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybdate reduction. Optimal conditions for molybdate reduction were between 30 and 50 mM molybdate, between 15 and 20°C, and initial pH between 6.5 and 7.5. The Mo-blue produced had a unique absorption spectrum with a peak maximum at 865 nm and a shoulder at 710 nm. Respiratory inhibitors such as antimycin A, sodium azide, potassium cyanide, and rotenone failed to inhibit the reducing activity. The Mo-reducing enzyme was partially purified using ion exchange and gel filtration chromatography. The partially purified enzyme showed optimal pH and temperature for activity at 6.0 and 20°C, respectively. Metal ions such as cadmium, chromium, copper, silver, lead, and mercury caused more than 95% inhibition of the molybdenum-reducing activity at 0.1 mM. The isolate was tentatively identified as Pseudomonas sp. strain DRY1 based on partial 16s rDNA molecular phylogenetic assessment and the Biolog microbial identification system. The characteristics of this strain would make it very useful in bioremediation works in the polar and temperate countries. Article in Journal/Newspaper Antarc* Antarctic Antarctica Hindawi Publishing Corporation Antarctic BioMed Research International 2013 1 10 |
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Open Polar |
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Hindawi Publishing Corporation |
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fthindawi |
language |
English |
topic |
Microbiology |
spellingShingle |
Microbiology S. A. Ahmad M. Y. Shukor N. A. Shamaan W. P. Mac Cormack M. A. Syed Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium |
topic_facet |
Microbiology |
description |
A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo6+ to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybdate reduction. Optimal conditions for molybdate reduction were between 30 and 50 mM molybdate, between 15 and 20°C, and initial pH between 6.5 and 7.5. The Mo-blue produced had a unique absorption spectrum with a peak maximum at 865 nm and a shoulder at 710 nm. Respiratory inhibitors such as antimycin A, sodium azide, potassium cyanide, and rotenone failed to inhibit the reducing activity. The Mo-reducing enzyme was partially purified using ion exchange and gel filtration chromatography. The partially purified enzyme showed optimal pH and temperature for activity at 6.0 and 20°C, respectively. Metal ions such as cadmium, chromium, copper, silver, lead, and mercury caused more than 95% inhibition of the molybdenum-reducing activity at 0.1 mM. The isolate was tentatively identified as Pseudomonas sp. strain DRY1 based on partial 16s rDNA molecular phylogenetic assessment and the Biolog microbial identification system. The characteristics of this strain would make it very useful in bioremediation works in the polar and temperate countries. |
format |
Article in Journal/Newspaper |
author |
S. A. Ahmad M. Y. Shukor N. A. Shamaan W. P. Mac Cormack M. A. Syed |
author_facet |
S. A. Ahmad M. Y. Shukor N. A. Shamaan W. P. Mac Cormack M. A. Syed |
author_sort |
S. A. Ahmad |
title |
Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium |
title_short |
Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium |
title_full |
Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium |
title_fullStr |
Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium |
title_full_unstemmed |
Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium |
title_sort |
molybdate reduction to molybdenum blue by an antarctic bacterium |
publisher |
BioMed Research International |
publishDate |
2013 |
url |
https://doi.org/10.1155/2013/871941 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
https://doi.org/10.1155/2013/871941 |
op_rights |
Copyright © 2013 S. A. Ahmad et al. |
op_doi |
https://doi.org/10.1155/2013/871941 |
container_title |
BioMed Research International |
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2013 |
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1 |
op_container_end_page |
10 |
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1766258533347622912 |