Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula
Volcanic regions contain a variety of environments suitable for extremophiles. This study was focused on assessing and exploiting the prokaryotic diversity of two microbial communities derived from different Kamchatkian thermal springs by metagenomic approaches. Samples were taken from a thermoacido...
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fthindawi:oai:hindawi.com:10.1155/2013/136714 2023-05-15T16:59:16+02:00 Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula Bernd Wemheuer Robert Taube Pinar Akyol Franziska Wemheuer Rolf Daniel 2013 https://doi.org/10.1155/2013/136714 en eng Archaea https://doi.org/10.1155/2013/136714 Copyright © 2013 Bernd Wemheuer et al. Research Article 2013 fthindawi https://doi.org/10.1155/2013/136714 2019-05-26T06:29:28Z Volcanic regions contain a variety of environments suitable for extremophiles. This study was focused on assessing and exploiting the prokaryotic diversity of two microbial communities derived from different Kamchatkian thermal springs by metagenomic approaches. Samples were taken from a thermoacidophilic spring near the Mutnovsky Volcano and from a thermophilic spring in the Uzon Caldera. Environmental DNA for metagenomic analysis was isolated from collected sediment samples by direct cell lysis. The prokaryotic community composition was examined by analysis of archaeal and bacterial 16S rRNA genes. A total number of 1235 16S rRNA gene sequences were obtained and used for taxonomic classification. Most abundant in the samples were members of Thaumarchaeota, Thermotogae, and Proteobacteria. The Mutnovsky hot spring was dominated by the Terrestrial Hot Spring Group, Kosmotoga, and Acidithiobacillus. The Uzon Caldera was dominated by uncultured members of the Miscellaneous Crenarchaeotic Group and Enterobacteriaceae. The remaining 16S rRNA gene sequences belonged to the Aquificae, Dictyoglomi, Euryarchaeota, Korarchaeota, Thermodesulfobacteria, Firmicutes, and some potential new phyla. In addition, the recovered DNA was used for generation of metagenomic libraries, which were subsequently mined for genes encoding lipolytic and proteolytic enzymes. Three novel genes conferring lipolytic and one gene conferring proteolytic activity were identified. Article in Journal/Newspaper Kamchatka Kamchatka Peninsula Hindawi Publishing Corporation Kamchatka Peninsula ENVELOPE(160.000,160.000,56.000,56.000) Archaea 2013 1 13 |
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Open Polar |
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Hindawi Publishing Corporation |
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fthindawi |
language |
English |
description |
Volcanic regions contain a variety of environments suitable for extremophiles. This study was focused on assessing and exploiting the prokaryotic diversity of two microbial communities derived from different Kamchatkian thermal springs by metagenomic approaches. Samples were taken from a thermoacidophilic spring near the Mutnovsky Volcano and from a thermophilic spring in the Uzon Caldera. Environmental DNA for metagenomic analysis was isolated from collected sediment samples by direct cell lysis. The prokaryotic community composition was examined by analysis of archaeal and bacterial 16S rRNA genes. A total number of 1235 16S rRNA gene sequences were obtained and used for taxonomic classification. Most abundant in the samples were members of Thaumarchaeota, Thermotogae, and Proteobacteria. The Mutnovsky hot spring was dominated by the Terrestrial Hot Spring Group, Kosmotoga, and Acidithiobacillus. The Uzon Caldera was dominated by uncultured members of the Miscellaneous Crenarchaeotic Group and Enterobacteriaceae. The remaining 16S rRNA gene sequences belonged to the Aquificae, Dictyoglomi, Euryarchaeota, Korarchaeota, Thermodesulfobacteria, Firmicutes, and some potential new phyla. In addition, the recovered DNA was used for generation of metagenomic libraries, which were subsequently mined for genes encoding lipolytic and proteolytic enzymes. Three novel genes conferring lipolytic and one gene conferring proteolytic activity were identified. |
format |
Article in Journal/Newspaper |
author |
Bernd Wemheuer Robert Taube Pinar Akyol Franziska Wemheuer Rolf Daniel |
spellingShingle |
Bernd Wemheuer Robert Taube Pinar Akyol Franziska Wemheuer Rolf Daniel Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
author_facet |
Bernd Wemheuer Robert Taube Pinar Akyol Franziska Wemheuer Rolf Daniel |
author_sort |
Bernd Wemheuer |
title |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_short |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_full |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_fullStr |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_full_unstemmed |
Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula |
title_sort |
microbial diversity and biochemical potential encoded by thermal spring metagenomes derived from the kamchatka peninsula |
publisher |
Archaea |
publishDate |
2013 |
url |
https://doi.org/10.1155/2013/136714 |
long_lat |
ENVELOPE(160.000,160.000,56.000,56.000) |
geographic |
Kamchatka Peninsula |
geographic_facet |
Kamchatka Peninsula |
genre |
Kamchatka Kamchatka Peninsula |
genre_facet |
Kamchatka Kamchatka Peninsula |
op_relation |
https://doi.org/10.1155/2013/136714 |
op_rights |
Copyright © 2013 Bernd Wemheuer et al. |
op_doi |
https://doi.org/10.1155/2013/136714 |
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Archaea |
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2013 |
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1 |
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13 |
_version_ |
1766051497384083456 |