Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro
Methylmercury (MeHg) is a ubiquitous environmental contaminant which bioaccumulates in marine biota. Fish constitute an important part of a balanced human diet contributing with health beneficial nutrients but may also contain contaminants such as MeHg. Interactions between the marine n-3 fatty acid...
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fthindawi:oai:hindawi.com:10.1155/2012/417652 2023-05-15T15:32:38+02:00 Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro O. J. Nøstbakken I. L. Bredal P. A. Olsvik T. S. Huang B. E. Torstensen 2012 https://doi.org/10.1155/2012/417652 en eng Journal of Biomedicine and Biotechnology https://doi.org/10.1155/2012/417652 Copyright © 2012 O. J. Nøstbakken et al. Toxicology Research Article 2012 fthindawi https://doi.org/10.1155/2012/417652 2019-05-26T00:16:40Z Methylmercury (MeHg) is a ubiquitous environmental contaminant which bioaccumulates in marine biota. Fish constitute an important part of a balanced human diet contributing with health beneficial nutrients but may also contain contaminants such as MeHg. Interactions between the marine n-3 fatty acids eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) with MeHg-induced toxicity were investigated. Different toxic and metabolic responses were studied in Atlantic salmon kidney (ASK) cell line and the mammalian kidney-derived HEK293 cell line. Both cell lines were preincubated with DHA or EPA prior to MeHg-exposure, and cell toxicity was assessed differently in the cell lines by MeHg-uptake in cells (ASK and HEK293), proliferation (HEK293 and ASK), apoptosis (ASK), oxidation of the red-ox probe roGFP (HEK293), and regulation of selected toxicological and metabolic transcriptional markers (ASK). DHA was observed to decrease the uptake of MeHg in HEK293, but not in ASK cells. DHA also increased, while EPA decreased, MeHg-induced apoptosis in ASK. MeHg exposure induced changes in selected metabolic and known MeHg biomarkers in ASK cells. Both DHA and MeHg, but not EPA, oxidized roGFP in HEK293 cells. In conclusion, marine n-3 fatty acids may ameliorate MeHg toxicity, either by decreasing apoptosis (EPA) or by reducing MeHg uptake (DHA). However, DHA can also augment MeHg toxicity by increasing oxidative stress and apoptosis when combined with MeHg. Article in Journal/Newspaper Atlantic salmon Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology 2012 1 13 |
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English |
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Toxicology |
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Toxicology O. J. Nøstbakken I. L. Bredal P. A. Olsvik T. S. Huang B. E. Torstensen Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
topic_facet |
Toxicology |
description |
Methylmercury (MeHg) is a ubiquitous environmental contaminant which bioaccumulates in marine biota. Fish constitute an important part of a balanced human diet contributing with health beneficial nutrients but may also contain contaminants such as MeHg. Interactions between the marine n-3 fatty acids eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) with MeHg-induced toxicity were investigated. Different toxic and metabolic responses were studied in Atlantic salmon kidney (ASK) cell line and the mammalian kidney-derived HEK293 cell line. Both cell lines were preincubated with DHA or EPA prior to MeHg-exposure, and cell toxicity was assessed differently in the cell lines by MeHg-uptake in cells (ASK and HEK293), proliferation (HEK293 and ASK), apoptosis (ASK), oxidation of the red-ox probe roGFP (HEK293), and regulation of selected toxicological and metabolic transcriptional markers (ASK). DHA was observed to decrease the uptake of MeHg in HEK293, but not in ASK cells. DHA also increased, while EPA decreased, MeHg-induced apoptosis in ASK. MeHg exposure induced changes in selected metabolic and known MeHg biomarkers in ASK cells. Both DHA and MeHg, but not EPA, oxidized roGFP in HEK293 cells. In conclusion, marine n-3 fatty acids may ameliorate MeHg toxicity, either by decreasing apoptosis (EPA) or by reducing MeHg uptake (DHA). However, DHA can also augment MeHg toxicity by increasing oxidative stress and apoptosis when combined with MeHg. |
format |
Article in Journal/Newspaper |
author |
O. J. Nøstbakken I. L. Bredal P. A. Olsvik T. S. Huang B. E. Torstensen |
author_facet |
O. J. Nøstbakken I. L. Bredal P. A. Olsvik T. S. Huang B. E. Torstensen |
author_sort |
O. J. Nøstbakken |
title |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_short |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_full |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_fullStr |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_full_unstemmed |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_sort |
effect of marine omega 3 fatty acids on methylmercury-induced toxicity in fish and mammalian cells in vitro |
publisher |
Journal of Biomedicine and Biotechnology |
publishDate |
2012 |
url |
https://doi.org/10.1155/2012/417652 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://doi.org/10.1155/2012/417652 |
op_rights |
Copyright © 2012 O. J. Nøstbakken et al. |
op_doi |
https://doi.org/10.1155/2012/417652 |
container_title |
Journal of Biomedicine and Biotechnology |
container_volume |
2012 |
container_start_page |
1 |
op_container_end_page |
13 |
_version_ |
1766363135229296640 |