Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus
Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors. The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence a...
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fthindawi:oai:hindawi.com:10.1155/2009/767893 2023-05-15T18:15:40+02:00 Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus Airong Jia Xiao-Hua Zhang 2009 https://doi.org/10.1155/2009/767893 en eng Journal of Biomedicine and Biotechnology https://doi.org/10.1155/2009/767893 Copyright © 2009 Airong Jia and Xiao-Hua Zhang. Immunology Research Article 2009 fthindawi https://doi.org/10.1155/2009/767893 2019-05-26T00:35:42Z Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors. The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates. Quantitative real-time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS). After turbot were challenged with Vibrio harveyi, the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours. These findings suggested that CXCR4 may play a significant role in the immune response of turbot. Article in Journal/Newspaper Scophthalmus maximus Turbot Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology 2009 1 8 |
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Hindawi Publishing Corporation |
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English |
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Immunology |
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Immunology Airong Jia Xiao-Hua Zhang Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
topic_facet |
Immunology |
description |
Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors. The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates. Quantitative real-time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS). After turbot were challenged with Vibrio harveyi, the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours. These findings suggested that CXCR4 may play a significant role in the immune response of turbot. |
format |
Article in Journal/Newspaper |
author |
Airong Jia Xiao-Hua Zhang |
author_facet |
Airong Jia Xiao-Hua Zhang |
author_sort |
Airong Jia |
title |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_short |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_full |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_fullStr |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_full_unstemmed |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_sort |
molecular cloning, characterization, and expression analysis of the cxcr4 gene from turbot: scophthalmus maximus |
publisher |
Journal of Biomedicine and Biotechnology |
publishDate |
2009 |
url |
https://doi.org/10.1155/2009/767893 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
https://doi.org/10.1155/2009/767893 |
op_rights |
Copyright © 2009 Airong Jia and Xiao-Hua Zhang. |
op_doi |
https://doi.org/10.1155/2009/767893 |
container_title |
Journal of Biomedicine and Biotechnology |
container_volume |
2009 |
container_start_page |
1 |
op_container_end_page |
8 |
_version_ |
1766188850574524416 |