Xenogenomics: Genomic Bioprospecting in Indigenous and Exotic Plants Through EST Discovery, cDNA Microarray-Based Expression Profiling and Functional Genomics
To date, the overwhelming majority of genomics programs in plants have been directed at model or crop plant species, meaning that very little of the naturally occurring sequence diversity found in plants is available for characterization and exploitation. In contrast, ‘xenogenomics’ refers to the di...
Published in: | Comparative and Functional Genomics |
---|---|
Main Authors: | , |
Format: | Conference Object |
Language: | English |
Published: |
Comparative and Functional Genomics
2005
|
Subjects: | |
Online Access: | https://doi.org/10.1002/cfg.475 |
id |
fthindawi:oai:hindawi.com:10.1002/cfg.475 |
---|---|
record_format |
openpolar |
spelling |
fthindawi:oai:hindawi.com:10.1002/cfg.475 2023-05-15T14:02:25+02:00 Xenogenomics: Genomic Bioprospecting in Indigenous and Exotic Plants Through EST Discovery, cDNA Microarray-Based Expression Profiling and Functional Genomics Ulrik P. John German C. Spangenberg 2005 https://doi.org/10.1002/cfg.475 en eng Comparative and Functional Genomics https://doi.org/10.1002/cfg.475 Copyright © 2005 Hindawi Publishing Corporation. Conference Paper 2005 fthindawi https://doi.org/10.1002/cfg.475 2019-05-26T01:08:06Z To date, the overwhelming majority of genomics programs in plants have been directed at model or crop plant species, meaning that very little of the naturally occurring sequence diversity found in plants is available for characterization and exploitation. In contrast, ‘xenogenomics’ refers to the discovery and functional analysis of novel genes and alleles from indigenous and exotic species, permitting bioprospecting of biodiversity using high-throughput genomics experimental approaches. Such a program has been initiated to bioprospect for genetic determinants of abiotic stress tolerance in indigenous Australian flora and native Antarctic plants. Uniquely adapted Poaceae and Fabaceae species with enhanced tolerance to salt, drought, elevated soil aluminium concentration, and freezing stress have been identified, based primarily on their eco-physiology, and have been subjected to structural and functional genomics analyses. For each species, EST collections have been derived from plants subjected to appropriate abiotic stresses. Transcript profiling with spotted unigene cDNA micro-arrays has been used to identify genes that are transcriptionally modulated in response to abiotic stress. Candidate genes identified on the basis of sequence annotation or transcript profiling have been assayed in planta and other in vivo systems for their capacity to confer novel phenotypes. Comparative genomics analysis of novel genes and alleles identified in the xenogenomics target plant species has subsequently been undertaken with reference to key model and crop plants. Conference Object Antarc* Antarctic Hindawi Publishing Corporation Antarctic Comparative and Functional Genomics 6 4 230 235 |
institution |
Open Polar |
collection |
Hindawi Publishing Corporation |
op_collection_id |
fthindawi |
language |
English |
description |
To date, the overwhelming majority of genomics programs in plants have been directed at model or crop plant species, meaning that very little of the naturally occurring sequence diversity found in plants is available for characterization and exploitation. In contrast, ‘xenogenomics’ refers to the discovery and functional analysis of novel genes and alleles from indigenous and exotic species, permitting bioprospecting of biodiversity using high-throughput genomics experimental approaches. Such a program has been initiated to bioprospect for genetic determinants of abiotic stress tolerance in indigenous Australian flora and native Antarctic plants. Uniquely adapted Poaceae and Fabaceae species with enhanced tolerance to salt, drought, elevated soil aluminium concentration, and freezing stress have been identified, based primarily on their eco-physiology, and have been subjected to structural and functional genomics analyses. For each species, EST collections have been derived from plants subjected to appropriate abiotic stresses. Transcript profiling with spotted unigene cDNA micro-arrays has been used to identify genes that are transcriptionally modulated in response to abiotic stress. Candidate genes identified on the basis of sequence annotation or transcript profiling have been assayed in planta and other in vivo systems for their capacity to confer novel phenotypes. Comparative genomics analysis of novel genes and alleles identified in the xenogenomics target plant species has subsequently been undertaken with reference to key model and crop plants. |
format |
Conference Object |
author |
Ulrik P. John German C. Spangenberg |
spellingShingle |
Ulrik P. John German C. Spangenberg Xenogenomics: Genomic Bioprospecting in Indigenous and Exotic Plants Through EST Discovery, cDNA Microarray-Based Expression Profiling and Functional Genomics |
author_facet |
Ulrik P. John German C. Spangenberg |
author_sort |
Ulrik P. John |
title |
Xenogenomics: Genomic Bioprospecting in Indigenous and Exotic Plants Through EST Discovery, cDNA Microarray-Based Expression Profiling and Functional Genomics |
title_short |
Xenogenomics: Genomic Bioprospecting in Indigenous and Exotic Plants Through EST Discovery, cDNA Microarray-Based Expression Profiling and Functional Genomics |
title_full |
Xenogenomics: Genomic Bioprospecting in Indigenous and Exotic Plants Through EST Discovery, cDNA Microarray-Based Expression Profiling and Functional Genomics |
title_fullStr |
Xenogenomics: Genomic Bioprospecting in Indigenous and Exotic Plants Through EST Discovery, cDNA Microarray-Based Expression Profiling and Functional Genomics |
title_full_unstemmed |
Xenogenomics: Genomic Bioprospecting in Indigenous and Exotic Plants Through EST Discovery, cDNA Microarray-Based Expression Profiling and Functional Genomics |
title_sort |
xenogenomics: genomic bioprospecting in indigenous and exotic plants through est discovery, cdna microarray-based expression profiling and functional genomics |
publisher |
Comparative and Functional Genomics |
publishDate |
2005 |
url |
https://doi.org/10.1002/cfg.475 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://doi.org/10.1002/cfg.475 |
op_rights |
Copyright © 2005 Hindawi Publishing Corporation. |
op_doi |
https://doi.org/10.1002/cfg.475 |
container_title |
Comparative and Functional Genomics |
container_volume |
6 |
container_issue |
4 |
container_start_page |
230 |
op_container_end_page |
235 |
_version_ |
1766272685709459456 |