Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins
Aspergillosis is a significant cause of mortality in captive penguins (Sphenisciformes). An indirect ELISA for the detection of Aspergillus fumigatus .specific immunoglobulin in penguins was developed and standardised by making use of a family.specific antiserum (anti- Aptenodyptes patagonica patago...
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2002
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fthighwire:oai:open-archive.highwire.org:vetrec:150/16/513 2023-05-15T17:03:56+02:00 Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins German, A. C. Flach, E. J. Shankland, G. S. Edwards, J. 2002-04-20 00:00:00.0 text/html http://veterinaryrecord.bmj.com/cgi/content/short/150/16/513 https://doi.org/10.1136/vr.150.16.513 en eng BMJ Publishing Group Ltd http://veterinaryrecord.bmj.com/cgi/content/short/150/16/513 http://dx.doi.org/10.1136/vr.150.16.513 Copyright (C) 2002, British Veterinary Association Papers & Articles TEXT 2002 fthighwire https://doi.org/10.1136/vr.150.16.513 2015-02-28T14:50:05Z Aspergillosis is a significant cause of mortality in captive penguins (Sphenisciformes). An indirect ELISA for the detection of Aspergillus fumigatus .specific immunoglobulin in penguins was developed and standardised by making use of a family.specific antiserum (anti- Aptenodyptes patagonica patagonicus ). The results were calculated quantitatively as ELISA units, derived by polynomial regression analysis, and semi.quantitatively as end titres. Serum samples from 61 captive penguins were tested with the assay, and the results were compared with those obtained by counterimmunoelectrophoresis (CIE). The ELISA results correlated with the CIE results only when end titres were reported (R s =-0.676, P<0.002). Fifty.seven of the penguins (93 per cent) were seropositive, but the detection of immunoglobulin did not correlate with clinical disease. At Whipsnade Wild Animal Park, Humboldt's penguins ( Spheniscus humboldti ) demonstrated higher seropositivity than king penguins ( Aptenodyptes patagonica patagonicus ) (P=0.022), but Humboldt's penguins at Fota Wildlife Park had a significantly higher seropositivity than Humboldt's penguins at Whipsnade (P=0.035). Text King Penguins HighWire Press (Stanford University) Veterinary Record 150 16 513 518 |
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HighWire Press (Stanford University) |
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Papers & Articles |
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Papers & Articles German, A. C. Flach, E. J. Shankland, G. S. Edwards, J. Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins |
topic_facet |
Papers & Articles |
description |
Aspergillosis is a significant cause of mortality in captive penguins (Sphenisciformes). An indirect ELISA for the detection of Aspergillus fumigatus .specific immunoglobulin in penguins was developed and standardised by making use of a family.specific antiserum (anti- Aptenodyptes patagonica patagonicus ). The results were calculated quantitatively as ELISA units, derived by polynomial regression analysis, and semi.quantitatively as end titres. Serum samples from 61 captive penguins were tested with the assay, and the results were compared with those obtained by counterimmunoelectrophoresis (CIE). The ELISA results correlated with the CIE results only when end titres were reported (R s =-0.676, P<0.002). Fifty.seven of the penguins (93 per cent) were seropositive, but the detection of immunoglobulin did not correlate with clinical disease. At Whipsnade Wild Animal Park, Humboldt's penguins ( Spheniscus humboldti ) demonstrated higher seropositivity than king penguins ( Aptenodyptes patagonica patagonicus ) (P=0.022), but Humboldt's penguins at Fota Wildlife Park had a significantly higher seropositivity than Humboldt's penguins at Whipsnade (P=0.035). |
format |
Text |
author |
German, A. C. Flach, E. J. Shankland, G. S. Edwards, J. |
author_facet |
German, A. C. Flach, E. J. Shankland, G. S. Edwards, J. |
author_sort |
German, A. C. |
title |
Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins |
title_short |
Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins |
title_full |
Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins |
title_fullStr |
Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins |
title_full_unstemmed |
Development of an indirect ELISA for the detection of serum antibodies to Aspergillus fumigatus in captive penguins |
title_sort |
development of an indirect elisa for the detection of serum antibodies to aspergillus fumigatus in captive penguins |
publisher |
BMJ Publishing Group Ltd |
publishDate |
2002 |
url |
http://veterinaryrecord.bmj.com/cgi/content/short/150/16/513 https://doi.org/10.1136/vr.150.16.513 |
genre |
King Penguins |
genre_facet |
King Penguins |
op_relation |
http://veterinaryrecord.bmj.com/cgi/content/short/150/16/513 http://dx.doi.org/10.1136/vr.150.16.513 |
op_rights |
Copyright (C) 2002, British Veterinary Association |
op_doi |
https://doi.org/10.1136/vr.150.16.513 |
container_title |
Veterinary Record |
container_volume |
150 |
container_issue |
16 |
container_start_page |
513 |
op_container_end_page |
518 |
_version_ |
1766057919532498944 |