Fasting Augments PCB Impact on Liver Metabolism in Anadromous Arctic Charr
Anadromous Arctic charr ( Salvelinus alpinus ) undertake short feeding migrations to seawater every summer and accumulates lipids, while the rest of the year is spent in fresh water where the accumulated lipid reserves are mobilized. We tested the hypothesis that winter fasting, and the associated p...
Published in: | Toxicological Sciences |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
Oxford University Press
2006
|
Subjects: | |
Online Access: | http://toxsci.oxfordjournals.org/cgi/content/short/kfj154v1 https://doi.org/10.1093/toxsci/kfj154 |
id |
fthighwire:oai:open-archive.highwire.org:toxsci:kfj154v1 |
---|---|
record_format |
openpolar |
spelling |
fthighwire:oai:open-archive.highwire.org:toxsci:kfj154v1 2023-05-15T14:30:02+02:00 Fasting Augments PCB Impact on Liver Metabolism in Anadromous Arctic Charr Vijayan, Mathilakath M. Aluru, Neelakanteswar Maule, Alec G. Jørgensen, Even H. 2006-03-14 06:32:40.0 text/html http://toxsci.oxfordjournals.org/cgi/content/short/kfj154v1 https://doi.org/10.1093/toxsci/kfj154 en eng Oxford University Press http://toxsci.oxfordjournals.org/cgi/content/short/kfj154v1 http://dx.doi.org/10.1093/toxsci/kfj154 Copyright (C) 2006, Society of Toxicology Environmental Toxicology TEXT 2006 fthighwire https://doi.org/10.1093/toxsci/kfj154 2013-05-26T13:20:52Z Anadromous Arctic charr ( Salvelinus alpinus ) undertake short feeding migrations to seawater every summer and accumulates lipids, while the rest of the year is spent in fresh water where the accumulated lipid reserves are mobilized. We tested the hypothesis that winter fasting, and the associated polychlorinated biphenyls (PCBs) redistribution from lipid depots to critical tissues impairs the liver metabolic capacity in these animals. Charr were administered Aroclor 1254 (0, 1, 10 and 100 mg /kg body mass) orally and maintained for 4 months without feeding to mimic seasonal winter fasting, while fed groups (0 and 100 mg Aroclor 1254/ kg) were maintained for comparison. A clear dose-related increase in PCB accumulation and CYP1A protein content were observed in the livers of fasted fish. This PCB concentration and CYP1A response with the high dose of Aroclor were 1.5-fold and 3-fold greater in the fasted than in the fed fish, respectively. In fed fish, PCB exposure lowered liver glycogen content, whereas none of the other metabolic indicators were significantly affected. In fasted fish, PCB exposure depressed liver glycogen content and activities of glucose-6-phosphate dehydrogenase, alanine aminotransferase, lactate dehydrogenase and phospho enol pyruvate carboxykinase, and elevated 3-hydoxyacylcoA dehydrogenase activity and glucocorticoid receptor protein expression. There were no significant impacts of PCB on hsp70 and hsp90 protein contents in either fed or fasted fish. Collectively, our study demonstrates that winter emaciation associated with the anadromous lifestyle predisposes Arctic charr to PCB impact on hepatic metabolism, including disruption of the adaptive metabolic responses to extended fasting. Text Arctic charr Arctic Salvelinus alpinus HighWire Press (Stanford University) Arctic Toxicological Sciences 91 2 431 439 |
institution |
Open Polar |
collection |
HighWire Press (Stanford University) |
op_collection_id |
fthighwire |
language |
English |
topic |
Environmental Toxicology |
spellingShingle |
Environmental Toxicology Vijayan, Mathilakath M. Aluru, Neelakanteswar Maule, Alec G. Jørgensen, Even H. Fasting Augments PCB Impact on Liver Metabolism in Anadromous Arctic Charr |
topic_facet |
Environmental Toxicology |
description |
Anadromous Arctic charr ( Salvelinus alpinus ) undertake short feeding migrations to seawater every summer and accumulates lipids, while the rest of the year is spent in fresh water where the accumulated lipid reserves are mobilized. We tested the hypothesis that winter fasting, and the associated polychlorinated biphenyls (PCBs) redistribution from lipid depots to critical tissues impairs the liver metabolic capacity in these animals. Charr were administered Aroclor 1254 (0, 1, 10 and 100 mg /kg body mass) orally and maintained for 4 months without feeding to mimic seasonal winter fasting, while fed groups (0 and 100 mg Aroclor 1254/ kg) were maintained for comparison. A clear dose-related increase in PCB accumulation and CYP1A protein content were observed in the livers of fasted fish. This PCB concentration and CYP1A response with the high dose of Aroclor were 1.5-fold and 3-fold greater in the fasted than in the fed fish, respectively. In fed fish, PCB exposure lowered liver glycogen content, whereas none of the other metabolic indicators were significantly affected. In fasted fish, PCB exposure depressed liver glycogen content and activities of glucose-6-phosphate dehydrogenase, alanine aminotransferase, lactate dehydrogenase and phospho enol pyruvate carboxykinase, and elevated 3-hydoxyacylcoA dehydrogenase activity and glucocorticoid receptor protein expression. There were no significant impacts of PCB on hsp70 and hsp90 protein contents in either fed or fasted fish. Collectively, our study demonstrates that winter emaciation associated with the anadromous lifestyle predisposes Arctic charr to PCB impact on hepatic metabolism, including disruption of the adaptive metabolic responses to extended fasting. |
format |
Text |
author |
Vijayan, Mathilakath M. Aluru, Neelakanteswar Maule, Alec G. Jørgensen, Even H. |
author_facet |
Vijayan, Mathilakath M. Aluru, Neelakanteswar Maule, Alec G. Jørgensen, Even H. |
author_sort |
Vijayan, Mathilakath M. |
title |
Fasting Augments PCB Impact on Liver Metabolism in Anadromous Arctic Charr |
title_short |
Fasting Augments PCB Impact on Liver Metabolism in Anadromous Arctic Charr |
title_full |
Fasting Augments PCB Impact on Liver Metabolism in Anadromous Arctic Charr |
title_fullStr |
Fasting Augments PCB Impact on Liver Metabolism in Anadromous Arctic Charr |
title_full_unstemmed |
Fasting Augments PCB Impact on Liver Metabolism in Anadromous Arctic Charr |
title_sort |
fasting augments pcb impact on liver metabolism in anadromous arctic charr |
publisher |
Oxford University Press |
publishDate |
2006 |
url |
http://toxsci.oxfordjournals.org/cgi/content/short/kfj154v1 https://doi.org/10.1093/toxsci/kfj154 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_relation |
http://toxsci.oxfordjournals.org/cgi/content/short/kfj154v1 http://dx.doi.org/10.1093/toxsci/kfj154 |
op_rights |
Copyright (C) 2006, Society of Toxicology |
op_doi |
https://doi.org/10.1093/toxsci/kfj154 |
container_title |
Toxicological Sciences |
container_volume |
91 |
container_issue |
2 |
container_start_page |
431 |
op_container_end_page |
439 |
_version_ |
1766303959488659456 |