Gestational Exposure to Persistent Organic Pollutants: Maternal Liver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dam and Fetus
Dietary exposure of Inuit people to a mixture of pesticides and polychlorinated biphenyls, or persistent organic pollutants (POPs), during pregnancy is a public health concern. We examined the consequences of administering the mixture of 28 POPs found in the Inuit diet (at doses representing 10-1000...
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2003
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fthighwire:oai:open-archive.highwire.org:toxsci:kfg023v1 2023-05-15T16:55:05+02:00 Gestational Exposure to Persistent Organic Pollutants: Maternal Liver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dam and Fetus Adeeko, Adedayo Li, Daming Doucet, Josée Cooke, Gerard M. Trasler, Jacquetta M. Robaire, Bernard Hales, Barbara F. 2003-03-07 12:02:38.0 text/html http://toxsci.oxfordjournals.org/cgi/content/short/kfg023v1 https://doi.org/10.1093/toxsci/kfg023 en eng Oxford University Press http://toxsci.oxfordjournals.org/cgi/content/short/kfg023v1 http://dx.doi.org/10.1093/toxsci/kfg023 Copyright (C) 2003, Society of Toxicology Environmental Toxicology TEXT 2003 fthighwire https://doi.org/10.1093/toxsci/kfg023 2013-05-26T13:18:03Z Dietary exposure of Inuit people to a mixture of pesticides and polychlorinated biphenyls, or persistent organic pollutants (POPs), during pregnancy is a public health concern. We examined the consequences of administering the mixture of 28 POPs found in the Inuit diet (at doses representing 10-1000 times dietary levels) by gavage to pregnant Sprague Dawley rats either during gestation days 0-19 or 8-19. The levels of individual components of the POPs mixture in the maternal liver were measured by high-resolution mass spectrometry. On gestation day 20, dams were sacrificed and pregnancy outcome determined. RNA isolated from maternal and fetal livers was 32P-labelled for gene expression profiling. The concentrations of individual POPs were increased in maternal livers of dams gavaged with the 1000X POPs mixture by 10 to 500-fold. While exposure to POPs had no significant effects on pregnancy outcome, dramatic changes were observed in the gene expression profiles of both the maternal and fetal livers. The gene expression profiles of maternal and fetal female and male liver were distinct with respect to the numbers of transcripts detected, the genes expressed exclusively in control or POPs-exposed livers, and those for which expression was up- or downregulated. While different genes were affected in each group, the overall consequence of POPs exposure on hepatic gene expression profiles was to decrease both the numbers of genes expressed and the relative intensity of expression. Thus, in utero exposure to POPs alters hepatic gene expression in the dam and the fetus; these changes may have functional implications. Text inuit HighWire Press (Stanford University) Toxicological Sciences 72 2 242 252 |
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HighWire Press (Stanford University) |
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English |
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Environmental Toxicology |
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Environmental Toxicology Adeeko, Adedayo Li, Daming Doucet, Josée Cooke, Gerard M. Trasler, Jacquetta M. Robaire, Bernard Hales, Barbara F. Gestational Exposure to Persistent Organic Pollutants: Maternal Liver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dam and Fetus |
topic_facet |
Environmental Toxicology |
description |
Dietary exposure of Inuit people to a mixture of pesticides and polychlorinated biphenyls, or persistent organic pollutants (POPs), during pregnancy is a public health concern. We examined the consequences of administering the mixture of 28 POPs found in the Inuit diet (at doses representing 10-1000 times dietary levels) by gavage to pregnant Sprague Dawley rats either during gestation days 0-19 or 8-19. The levels of individual components of the POPs mixture in the maternal liver were measured by high-resolution mass spectrometry. On gestation day 20, dams were sacrificed and pregnancy outcome determined. RNA isolated from maternal and fetal livers was 32P-labelled for gene expression profiling. The concentrations of individual POPs were increased in maternal livers of dams gavaged with the 1000X POPs mixture by 10 to 500-fold. While exposure to POPs had no significant effects on pregnancy outcome, dramatic changes were observed in the gene expression profiles of both the maternal and fetal livers. The gene expression profiles of maternal and fetal female and male liver were distinct with respect to the numbers of transcripts detected, the genes expressed exclusively in control or POPs-exposed livers, and those for which expression was up- or downregulated. While different genes were affected in each group, the overall consequence of POPs exposure on hepatic gene expression profiles was to decrease both the numbers of genes expressed and the relative intensity of expression. Thus, in utero exposure to POPs alters hepatic gene expression in the dam and the fetus; these changes may have functional implications. |
format |
Text |
author |
Adeeko, Adedayo Li, Daming Doucet, Josée Cooke, Gerard M. Trasler, Jacquetta M. Robaire, Bernard Hales, Barbara F. |
author_facet |
Adeeko, Adedayo Li, Daming Doucet, Josée Cooke, Gerard M. Trasler, Jacquetta M. Robaire, Bernard Hales, Barbara F. |
author_sort |
Adeeko, Adedayo |
title |
Gestational Exposure to Persistent Organic Pollutants: Maternal Liver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dam and Fetus |
title_short |
Gestational Exposure to Persistent Organic Pollutants: Maternal Liver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dam and Fetus |
title_full |
Gestational Exposure to Persistent Organic Pollutants: Maternal Liver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dam and Fetus |
title_fullStr |
Gestational Exposure to Persistent Organic Pollutants: Maternal Liver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dam and Fetus |
title_full_unstemmed |
Gestational Exposure to Persistent Organic Pollutants: Maternal Liver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dam and Fetus |
title_sort |
gestational exposure to persistent organic pollutants: maternal liver residues, pregnancy outcome, and effects on hepatic gene expression profiles in the dam and fetus |
publisher |
Oxford University Press |
publishDate |
2003 |
url |
http://toxsci.oxfordjournals.org/cgi/content/short/kfg023v1 https://doi.org/10.1093/toxsci/kfg023 |
genre |
inuit |
genre_facet |
inuit |
op_relation |
http://toxsci.oxfordjournals.org/cgi/content/short/kfg023v1 http://dx.doi.org/10.1093/toxsci/kfg023 |
op_rights |
Copyright (C) 2003, Society of Toxicology |
op_doi |
https://doi.org/10.1093/toxsci/kfg023 |
container_title |
Toxicological Sciences |
container_volume |
72 |
container_issue |
2 |
container_start_page |
242 |
op_container_end_page |
252 |
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1766046065786617856 |