Improved activity and thermostability of Candida antarctica lipase B by DNA family shuffling

DNA family shuffling was used to create chimeric lipase B proteins with improved activity toward the hydrolysis of diethyl 3‐(3′,4′‐dichlorophenyl)glutarate (DDG). Three homologous lipases from Candida antarctica ATCC 32657, Hyphozyma sp. CBS 648.91 and Crytococcus tsukubaensis ATCC 24555 were clone...

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Bibliographic Details
Published in:Protein Engineering Design and Selection
Main Authors: Suen, Wen-Chen, Zhang, Ningyan, Xiao, Li, Madison, Vincent, Zaks, Aleksey
Format: Text
Language:English
Published: Oxford University Press 2004
Subjects:
ORIGINAL ARTICLES
Antarc*
Antarctica
Online Access:http://peds.oxfordjournals.org/cgi/content/short/17/2/133
https://doi.org/10.1093/protein/gzh017
Description
Summary:DNA family shuffling was used to create chimeric lipase B proteins with improved activity toward the hydrolysis of diethyl 3‐(3′,4′‐dichlorophenyl)glutarate (DDG). Three homologous lipases from Candida antarctica ATCC 32657, Hyphozyma sp. CBS 648.91 and Crytococcus tsukubaensis ATCC 24555 were cloned and shuffled to generate a diverse gene library. A high‐throughput screening assay was developed and used successfully to identify chimeric lipase B proteins having a 20‐fold higher activity toward DDG than lipase B from C.antarctica ATCC 32657 and a 13‐fold higher activity than the most active parent derived from C.tsukubaensis ATCC 24555. In addition, the stability characteristics of several highly active chimeric proteins were also improved as a result of family shuffling. For example, the half‐life at 45°C and melting point ( T m ) of one chimera exceeded those of lipase B from C.antarctica ATCC 32657 by 11‐fold and 6.4°C, respectively, which closely approached the stability characteristics of the most thermostable parent derived from Hyphozyma sp. CBS 648.91.

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